Identification of wheat DREB genes and functional characterization of TaDREB3 in response to abiotic stresses

Niu, Xinhuan; Luo, Tengli; Zhao, Hongyan; Su, Yali; Ji, Wanquan; Li, Haifeng

doi:10.1016/j.gene.2020.144514

Cited by 54 publications

(46 citation statements)

References 85 publications

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“…Dehydration responsive element-binding (DREB) transcription factors play a crucial role in plant growth, development, and stress responses; therefore, we tested all 210 genes from the DREB family indicated by Niu et al (2020). Among them, we identified 12 genes as DEGs for cv.…”

Section: Discussionmentioning

confidence: 99%

Comparative transcriptome profiling of a resistant vs susceptible bread wheat (Triticum aestivum L.) cultivar in response to water deficit and cold stress

Konstantinov

Зубаирова

Ermakov

et al. 2021

PeerJ

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Bread wheat (Triticum aestivum L.) is one of the most important agricultural plants wearing abiotic stresses, such as water deficit and cold, that cause its productivity reduction. Since resistance to abiotic factors is a multigenic trait, therefore modern genome-wide approaches can help to involve various genetic material in breeding. One technique is full transcriptome analysis that reveals groups of stress response genes serving marker-assisted selection markers. Comparing transcriptome profiles of the same genetic material under several stresses is essential and makes the whole picture. Here, we addressed this by studying the transcriptomic response to water deficit and cold stress for two evolutionarily distant bread wheat varieties: stress-resistant cv. Saratovskaya 29 (S29) and stress-sensitive cv. Yanetzkis Probat (YP). For the first time, transcriptomes for these cultivars grown under abiotic stress conditions were obtained using Illumina based MACE technology. We identified groups of genes involved in response to cold and water deficiency stresses, including responses to each stress factor and both factors simultaneously that may be candidates for resistance genes. We discovered a core group of genes that have a similar pattern of stress-induced expression changes. The particular expression pattern was revealed not only for the studied varieties but also for the published transcriptomic data on cv. Jing 411 and cv. Fielder. Comparative transcriptome profiling of cv. S29 and cv. YP in response to water deficit and cold stress confirmed the hypothesis that stress-induced expression change is unequal within a homeologous gene group. As a rule, at least one changed significantly while the others had a relatively lower expression. Also, we found several SNPs distributed throughout the genomes of cv. S29 and cv. YP and distinguished the studied varieties from each other and the reference cv. Chinese Spring. Our results provide new data for genomics-assisted breeding of stress-tolerant wheat cultivars.

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Section: Discussionmentioning

confidence: 99%

Comparative transcriptome profiling of a resistant vs susceptible bread wheat (Triticum aestivum L.) cultivar in response to water deficit and cold stress

Konstantinov

Зубаирова

Ermakov

et al. 2021

PeerJ

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“…Arabidopsis thaliana is often used as a model plant to study gene functions due to its short growth cycle, small nuclear genome, and high reproduction coefficient. Niu et al constructed over-expression TaDREB3 transgenic Arabidopsis plants to explore the function of the TaDREB3 gene under drought, salt, and heat stresses [ 34 ]. Over-expression of TaWRKY46 enhanced osmotic stress tolerance in transgenic Arabidopsis thaliana plants, which was mainly demonstrated by transgenic Arabidopsis plants forming a higher germination rate and longer root length [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

Cytosolic TaGAPC2 Enhances Tolerance to Drought Stress in Transgenic Arabidopsis Plants

Zhang

Yang

2020

IJMS

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Drought is a major natural disaster that seriously affects agricultural production, especially for winter wheat in boreal China. As functional proteins, the functions and mechanisms of glyceraldehyde-3-phosphate dehydrogenase in cytoplasm (GAPCs) have remained little investigated in wheat subjected to adverse environmental conditions. In this study, we cloned and characterized a GAPC isoform TaGAPC2 in wheat. Over-expression of TaGApC2-6D in Arabidopsis led to enhanced root length, reduced reactive oxygen species (ROS) production, and elevated drought tolerance. In addition, the dual-luciferase assays showed that TaWRKY28/33/40/47 could positively regulate the expression of TaGApC2-6A and TaGApC2-6D. Further results of the yeast two-hybrid system and bimolecular fluorescence complementation assay (BiFC) demonstrate that TaPLDδ, an enzyme producing phosphatidic acid (PA), could interact with TaGAPC2-6D in plants. These results demonstrate that TaGAPC2 regulated by TaWRKY28/33/40/47 plays a crucial role in drought tolerance, which may influence the drought stress conditions via interaction with TaPLDδ. In conclusion, our results establish a new positive regulation mechanism of TaGAPC2 that helps wheat fine-tune its drought response.

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“…The identified AP2/ERF TFs, known to be involved in abiotic tolerance, belonged to Arabidopsis [132]. Overexpression of wheat TaDREB3-A1 gene displayed the enhanced tolerance in transgenic Arabidopsis plants against heat, drought and salt stresses [133]. An observation revealed that Arabidopsis plants overexpressing RAP2.1 showed an enhanced sensitivity to cold and drought stresses, as evidenced that RAP2.1 acting as an active transcriptional repressor in defense responses to these stresses in Arabidopsis through the down-expression of the desiccation/Cold-regulated (RD/COR) genes [134].…”

Section: Function and Expression Pattern Of Ap2/erf Tfs Under Abioticmentioning

confidence: 99%

Transcription Factors in Plant Stress Responses: Challenges and Potential for Sugarcane Improvement

Javed

Shabbir

Ali

et al. 2020

Plants

174

107

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Increasing vulnerability of crops to a wide range of abiotic and biotic stresses can have a marked influence on the growth and yield of major crops, especially sugarcane (Saccharum spp.). In response to various stresses, plants have evolved a variety of complex defense systems of signal perception and transduction networks. Transcription factors (TFs) that are activated by different pathways of signal transduction and can directly or indirectly combine with cis-acting elements to modulate the transcription efficiency of target genes, which play key regulators for crop genetic improvement. Over the past decade, significant progresses have been made in deciphering the role of plant TFs as key regulators of environmental responses in particular important cereal crops; however, a limited amount of studies have focused on sugarcane. This review summarizes the potential functions of major TF families, such as WRKY, NAC, MYB and AP2/ERF, in regulating gene expression in the response of plants to abiotic and biotic stresses, which provides important clues for the engineering of stress-tolerant cultivars in sugarcane.

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Identification of wheat DREB genes and functional characterization of TaDREB3 in response to abiotic stresses

Cited by 54 publications

References 85 publications

Comparative transcriptome profiling of a resistant vs susceptible bread wheat (Triticum aestivum L.) cultivar in response to water deficit and cold stress

Comparative transcriptome profiling of a resistant vs susceptible bread wheat (Triticum aestivum L.) cultivar in response to water deficit and cold stress

Cytosolic TaGAPC2 Enhances Tolerance to Drought Stress in Transgenic Arabidopsis Plants

Transcription Factors in Plant Stress Responses: Challenges and Potential for Sugarcane Improvement

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