Identification of WHIRLY1 as a Factor Binding to the Promoter of the Stress- and Senescence-Associated Gene HvS40

Krupinska, Karin; Dähnhardt, Dorothee; Fischer-Kilbienski, Isabell; Kucharewicz, Weronika; Scharrenberg, Christian; Trösch, Mirl; Buck, Friedrich

doi:10.1007/s00344-013-9378-9

Cited by 40 publications

(62 citation statements)

References 76 publications

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“…This transcript is identical to the barley organelle DNA polymerase described recently (Krupinska et al, 2014a(Krupinska et al, , 2014b and homologous to the AtPol1B (At3g20540) sequence. Similarly, a microtubule end binding protein 1A (MLOC 52339.1) and a transcript encoding a protein containing a B3 DNA binding domain (AK251585.1) that is found exclusively in transcription factors were significantly more abundant in W1-7 leaves relative to the wild type ( Fig.…”

Section: The Transcript Profile Is Modified In Whirly1-deficient Leavesmentioning

confidence: 79%

“…It is required for both expression of SA-regulated genes and associated disease resistance, although this pathway is independent of NONEXPRESSOR OF PATHOGENESIS-RELATED PROTEIN1 (Desveaux et al, 2004). The barley (Hordeum vulgare) WHIRLY1 protein binds to the promoter of the senescence-associated gene HvS40, which is induced in natural and stress-induced senescence (Krupinska et al, 2002(Krupinska et al, , 2014a.…”

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confidence: 99%

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WHIRLY1 Functions in the Control of Responses to Nitrogen Deficiency But Not Aphid Infestation in Barley

et al. 2015

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WHIRLY1 is largely targeted to plastids, where it is a major constituent of the nucleoids. To explore WHIRLY1 functions in barley (Hordeum vulgare), RNA interference-knockdown lines (W1-1, W1-7, and W1-9) that have very low levels of HvWHIRLY1 transcripts were characterized in plants grown under optimal and stress conditions. The WHIRLY1-1 (W1-1), W1-7, and W1-9 plants were phenotypically similar to the wild type but produced fewer tillers and seeds. Photosynthesis rates were similar in all lines, but W1-1, W1-7, and W1-9 leaves had significantly more chlorophyll and less sucrose than the wild type. Transcripts encoding specific subsets of chloroplast-localized proteins, such as ribosomal proteins, subunits of the RNA polymerase, and thylakoid nicotinamide adenine dinucleotide (reduced) and cytochrome b 6 /f complexes, were much more abundant in the W1-7 leaves than the wild type. Although susceptibility of aphid (Myzus persicae) infestation was similar in all lines, the WHIRLY1-deficient plants showed altered responses to nitrogen deficiency, maintaining higher photosynthetic CO 2 assimilation rates than the wild type under limiting nitrogen. Although all lines showed globally similar low nitrogen-dependent changes in transcripts and metabolites, the increased abundance of FAR-RED IMPAIRED RESPONSE1-like transcripts in nitrogen-deficient W1-7 leaves infers that WHIRLY1 has a role in communication between plastid and nuclear genes encoding photosynthetic proteins during abiotic stress.

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Section: The Transcript Profile Is Modified In Whirly1-deficient Leavesmentioning

confidence: 79%

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confidence: 99%

WHIRLY1 Functions in the Control of Responses to Nitrogen Deficiency But Not Aphid Infestation in Barley

et al. 2015

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“…In the nucleus, WHY1 (designated as PBF-2) was initially discovered as a transcriptional activator binding to the elicitor response element (ERE, TGACAnnnnTGTCA) in the promoter region of PR-10a (pathogenesis-related gene) to participate in the salicylic acid (SA)dependent disease resistance pathway (Desveaux et al, 2000(Desveaux et al, , 2004. In addition, WHY1 was found to regulate the expression of two senescence-associated marker genes HvS40 and AtWRKY53 in higher plants (Miao et al, 2013;Krupinska et al, 2014b). While HvWHY1 binds to the sequence TGTCAnnnnnGGTCAA in the promoter of HvS40 in barely (Hordeum vulgare), AtWHY1 associates with GNNNAAATT plus an AT-rich telomeric repeatlike sequence in the promoter of AtWRKY53 in Arabidopsis (Miao et al, 2013;Krupinska et al, 2014b).…”

Section: Introductionmentioning

confidence: 99%

“…In addition, WHY1 was found to regulate the expression of two senescence-associated marker genes HvS40 and AtWRKY53 in higher plants (Miao et al, 2013;Krupinska et al, 2014b). While HvWHY1 binds to the sequence TGTCAnnnnnGGTCAA in the promoter of HvS40 in barely (Hordeum vulgare), AtWHY1 associates with GNNNAAATT plus an AT-rich telomeric repeatlike sequence in the promoter of AtWRKY53 in Arabidopsis (Miao et al, 2013;Krupinska et al, 2014b). Recently, it was found that AtWHY1 can also be phosphorylated by the CIPK14 kinase, leading to the accumulation of phosphorylated WHY1 in the nucleus, where WHY1 binds to the promoter of WRKY53 and regulates leaf senescence .…”

Section: Introductionmentioning

confidence: 99%

Whirly1 enhances tolerance to chilling stress in tomato via protection of photosystem II and regulation of starch degradation

et al. 2018

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Summary In plants, the chilling response involves decreased photosynthetic capacity and increased starch accumulation in chloroplasts. However, the mechanisms that modulate these processes remain unclear. We found that the SlWHY1 gene is significantly induced by chilling stress (4°C) in tomato. Three SlWHY1 overexpression (OE) lines grew better than the wild type (WT) under chilling stress; the OE plants retained intact photosynthetic grana lamellae and showed enhanced hydrolysis of starch. By contrast, RNAi lines that inhibited SlWHY1 were more affected than the corresponding WT cultivar. Their grana lamellae were damaged and starch content increased. The psbA gene encodes the key photosystem II (PSII) protein D1. We show that SlWHY1 binds to the upstream region (A/GTTACCCT/A) of SlpsbA and enhances the de novo synthesis of D1 in chloroplasts. Additionally, SlWHY1 regulates the expression of the starch‐degrading enzyme α‐amylase (SlAMY3‐L) and the starch synthesis‐related enzyme isoamylase gene (SlISA2) in the nucleus, thus modulating the starch content in chloroplasts. We demonstrate that SlWHY1 enhances the resistance of tomato to chilling stress by maintaining the function of PSII and degrading starch. Thus, overexpression of WHY1 may be an effective strategy for enhancing resistance to chilling stress of chilling‐sensitive crops in agricultural production.

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“…WHY proteins are first discovered as nuclear transcriptional activator binding at elicitor response element in the promoter regions of pathogenesis-related genes in potato (Solanum tuberosum) and Arabidopsis [14,15]. Later it is found that they are able to bind to various DNA sequences, including telomeres [16], a distal element upstream of a kinesin gene [17], the promoter region of the early senescence marker gene WRKY53 in a development-dependent manner in Arabidopsis [9], and the promoter region of the senescence-associated gene HvS40, which was induced during natural and stress-related senescence in barley [18]. WHY1 is also proposed to bind to both ssDNA and RNA with a role in intron splicing in maize (Zea mays) chloroplasts [19], while in barley chloroplasts WHY1 is associated with intron-containing RNA [20].…”

Section: Introductionmentioning

confidence: 99%

Comparative Proteomics Analysis of Coregulation of CIPK14 and WHIRLY1/3 Mediated Leaf Pale Yellowing in <em>Arabidopsis</em>

Guan¹,

Wan-zhen²,

Yu³

et al. 2018

Preprint

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Leaf variegation pale yellowing is observed in the Calcineurin B-Like-Interacting Protein Kinase14 (CIPK14) overexpression line (oeCIPK14) and double knockout WHIRLY1/WHIRLY3 (why1/3) lines of Arabidopsis, the distribution of WHIRLY1 (WHY1) protein between plastids and the nucleus are affected by the phosphorylation of WHY1 by CIPK14. To elucidate the coregulation of CIPK14 and WHIRLY1/WHIRLY3 mediated leaf pale yellowing, a differential proteomic analysis is conducted between the oeCIPK14 variegated (oeCIPK14-var) line, why1/3 variegated (why1/3-var) line and wild type (WT). More than 800 protein spots are distinguished on each gel, 67 differential abundance proteins (DAPs) are identified by matrix-assisted laser desorption ionization-time of flight/time of flight mass spectrometry (MALDI-TOF/TOF-MS), of which, 34 DAPs are in the oeCIPK14-var, 33 DAPs are in the why1/3-var compared to WT. Five overlapping proteins differentially change both in the oeCIPK14-var and in the why1/3-var. They are ATP-dependent Clp protease proteolytic subunit-related protein 3 (ClpR3), Ribulose bisphosphate carboxylase large chain (RBL), Beta-amylase 3 (BAM3), Ribosome-recycling factor (RRF), Ribulose bisphosphate carboxylase small chain (RBS). Bioinformatics analysis show that most of DAPs are involved in photosynthesis, defense and antioxidation pathway, protein metabolism, amino acid metabolism, energy metabolism, malate biosynthesis, lipid metabolism and transcription. Thus, the photosystem parameters are measured that the content of chlorophyll, the photochemical efficiency of PSⅡ (Fv/Fm), and electron transport rates (ETR) decrease in the why1/3-var and oeCIPK14-var, but the non-photochemical quenching (NPQ) increases. Both mutants show high sensitivity to strong light. Based on the annotation of DAPs from both why1/3-var and oeCIPK14-var lines, we conclude that CIPK14 phosphorylation mediated WHY1 deficiency in plastids is related to impairment of protein metabolism leading to chloroplast dysfunction.

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Identification of WHIRLY1 as a Factor Binding to the Promoter of the Stress- and Senescence-Associated Gene HvS40

Cited by 40 publications

References 76 publications

WHIRLY1 Functions in the Control of Responses to Nitrogen Deficiency But Not Aphid Infestation in Barley

WHIRLY1 Functions in the Control of Responses to Nitrogen Deficiency But Not Aphid Infestation in Barley

Whirly1 enhances tolerance to chilling stress in tomato via protection of photosystem II and regulation of starch degradation

Comparative Proteomics Analysis of Coregulation of CIPK14 and WHIRLY1/3 Mediated Leaf Pale Yellowing in <em>Arabidopsis</em>

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