Identification of Wnt Genes Expressed in Neural Progenitor Zones during Zebrafish Brain Development

Duncan, Robert N.; Panahi, Samin; Piotrowski, Tatjana; Dorsky, Richard I.

doi:10.1371/journal.pone.0145810

Cited by 40 publications

(39 citation statements)

References 36 publications

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“…Pre-hybridization was performed for at least 2 hrs at 65°C. Probes used were fgfr1a, fgf3, fgf10a, and etv4 (Aman and Piotrowski, 2009), fgfr2 (Rohs et al, 2013), dld (Jiang et al, 2014), wnt2 (Poulain and Ober, 2011), wnt10a (Lush and Piotrowski, 2014b), wnt11r (Duncan et al, 2015), gfp (Dorsky et al, 2002), atoh1a…”

Section: In Situ Hybridization and Alkaline Phosphatase Stainingmentioning

confidence: 99%

Single cell RNA-Seq reveals distinct stem cell populations that drive sensory hair cell regeneration in response to loss of Fgf and Notch signaling

Koenecke

et al. 2018

Preprint

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Loss of sensory hair cells leads to deafness and balance deficiencies. In contrast to mammalian hair cells, zebrafish ear and lateral line hair cells regenerate from poorly characterized, proliferating support cells. Equally ill-defined is the gene regulatory network underlying the progression of support cells to cycling hair cell progenitors and differentiated hair cells. We used single cell RNA-Sequencing (scRNA-Seq) of lateral line sensory organs and uncovered five different support cell types, including quiescent and activated stem cells. In silico ordering of support cells along a developmental trajectory identified cells that self-renew and new groups of genes required for hair cell differentiation. scRNA-Seq analyses of fgf3 mutants, in which hair cell regeneration is increased, demonstrates that Fgf and Notch signaling inhibit proliferation of support cells in parallel by inhibiting Wnt signaling. Our scRNA-Seq analyses set the foundation for mechanistic studies of sensory organ regeneration and is crucial for identifying factors to trigger hair cell production in mammals. As a resource, we implemented a shiny application that allows the community to interrogate cell type specific expression of genes of interest.

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Section: In Situ Hybridization and Alkaline Phosphatase Stainingmentioning

confidence: 99%

Single cell RNA-Seq reveals distinct stem cell populations that drive sensory hair cell regeneration in response to loss of Fgf and Notch signaling

Koenecke

et al. 2018

Preprint

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“…The function of NE in the regenerative condition and RG in both, physiological and regenerative conditions, are not well known. In the previous studies, we analyzed the function of Wnt signaling on adult neurogenesis in the optic tectum of zebrafish (Shitasako et al, ), and Wnt‐related genes were expressed in the central nervous system of the zebrafish embryo (Clements, Ong, & Traver, ; Duncan, Panahi, Piotrowski, & Dorsky, ; Hashimoto et al, ; Untergasser, Martowicz, Hermann, Töchterle, & Meyer, ). Moreover, other studies also showed that Wnt signaling regulates self‐renewal and differentiation of neural stem cells in the physiological and regenerative conditions in the retina, hypothalamus, and spinal cord (McPherson et al, ; Ramachandran, Zhao, & Goldman, ; Strand et al, ).…”

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

Wnt signaling regulates proliferation and differentiation of radial glia in regenerative processes after stab injury in the optic tectum of adult zebrafish

Shimizu

Ueda

Ohshima

2018

Glia

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Zebrafish have superior abilities to generate new neurons in the adult brain and to regenerate brain tissue after brain injury compared with mammals. There exist two types of neural stem cells (NSCs): neuroepithelial-like stem cells (NE) and radial glia (RG) in the optic tectum. We established an optic tectum stab injury model to analyze the function of NSCs in the regenerative condition and confirmed that the injury induced the proliferation of RG, but not NE and that the proliferated RG differentiated into new neurons after the injury. We then analyzed the involvement of Wnt signaling after the injury, using a Wnt reporter line in which canonical Wnt signaling activation induced GFP expression and confirmed that GFP expression was induced specifically in RG after the injury. We also analyzed the expression level of genes related to Wnt signaling, and confirmed that endogenous Wnt antagonist dkk1b expression was significantly decreased after the injury. We observed that Wnt signal inhibitor IWR1 treatment suppressed the proliferation and differentiation of RG after the injury, suggesting that up-regulation of Wnt signaling in RG after the stab injury was required for optic tectum regeneration. We also confirmed that Wnt activation by treatment with GSK3β inhibitor BIO in uninjured zebrafish induced proliferation of RG in the optic tectum. This optic tectum stab injury model is useful for the study of the molecular mechanisms of brain regeneration and analysis of the RG functions in physiological and regenerative conditions.

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“…38 Canonical Wnt in the roof plate in gnathostomes and tailbud in amphioxus, no data on lamprey. References: Gnathostomes; Mouse, Wnt1, Wnt3, and Wnt3a in the roof plate 39,40 ; Chick, Wnt1 and Wnt3a 41 ; Zebrafish, Wnt1 and Wnt3 in the roof plate, 42,43 Wnt3a in the tailbud. 44 Lamprey; data only on noncanonical Wnt5 which is expressed across the DV spinal cord.…”

Section: Patterning the Vertebrate Spinal Cord: The Ap Axismentioning

confidence: 99%

Evolution of vertebrate spinal cord patterning

Leung

Shimeld

2019

Developmental Dynamics

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The vertebrate spinal cord is organized across three developmental axes, anterior‐posterior (AP), dorsal‐ventral (DV), and medial‐lateral (ML). Patterning of these axes is regulated by canonical intercellular signaling pathways: the AP axis by Wnt, fibroblast growth factor, and retinoic acid (RA), the DV axis by Hedgehog, Tgfβ, and Wnt, and the ML axis where proliferation is controlled by Notch. Developmental time plays an important role in which signal does what and when. Patterning across the three axes is not independent, but linked by interactions between signaling pathway components and their transcriptional targets. Combined this builds a sophisticated organ with many different types of cell in specific AP, DV, and ML positions. Two living lineages share phylum Chordata with vertebrates, amphioxus, and tunicates, while the jawless fish such as lampreys, survive as the most basally divergent vertebrate lineage. Genes and mechanisms shared between lampreys and other vertebrates tell us what predated vertebrates, while those also shared with other chordates tell us what evolved early in chordate evolution. Between these lie vertebrate innovations: genetic and developmental changes linked to evolution of new morphology. These include gene duplications, differences in how signals are received, and new regulatory connections between signaling pathways and their target genes.

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Identification of Wnt Genes Expressed in Neural Progenitor Zones during Zebrafish Brain Development

Cited by 40 publications

References 36 publications

Single cell RNA-Seq reveals distinct stem cell populations that drive sensory hair cell regeneration in response to loss of Fgf and Notch signaling

Single cell RNA-Seq reveals distinct stem cell populations that drive sensory hair cell regeneration in response to loss of Fgf and Notch signaling

Wnt signaling regulates proliferation and differentiation of radial glia in regenerative processes after stab injury in the optic tectum of adult zebrafish

Evolution of vertebrate spinal cord patterning

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