2015
DOI: 10.1128/jb.00238-15
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Identification of YsaP, the Pilotin of the Yersinia enterocolitica Ysa Type III Secretion System

Abstract: Secretins are multimeric outer membrane pore-forming proteins found in complex export systems in Gram-negative bacteria. All type III secretion systems (T3SSs) have a secretin, and one of these is the YsaC secretin of the chromosomally encoded Ysa T3SS of Yersinia enterocolitica. In some cases, pilotin proteins, which are outer membrane lipoproteins, are required for their cognate secretins to multimerize and/or localize to the outer membrane. However, if secretin multimers mislocalize to the inner membrane, t… Show more

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Cited by 6 publications
(10 citation statements)
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“…Klebsiella oxytoca PulD secretin multimers inserted into the E. coli IM were visualized by microscopy, and this event was correlated with increased PspA synthesis (34). Furthermore, in some cases, pilotin proteins facilitate secretin insertion into the OM (57), but those secretins become more potent Psp inducers when their pilotin is absent (e.g., 17,37,79). Other important observations have also implicated the IM as the site of Psp induction.…”
Section: The Location Of the Inducing Stimulusmentioning
confidence: 95%
“…Klebsiella oxytoca PulD secretin multimers inserted into the E. coli IM were visualized by microscopy, and this event was correlated with increased PspA synthesis (34). Furthermore, in some cases, pilotin proteins facilitate secretin insertion into the OM (57), but those secretins become more potent Psp inducers when their pilotin is absent (e.g., 17,37,79). Other important observations have also implicated the IM as the site of Psp induction.…”
Section: The Location Of the Inducing Stimulusmentioning
confidence: 95%
“…We therefore screened for additional interactors of SctG by co-immunoprecipitation. Notably, while the pilotin mostly fractionates with the OM in membrane preparations [18,25,59], many of the proteins found to interact with the labeled pilotin in the co-immunoprecipitation assay are integral IM proteins or IM-associated proteins. The used SctG-sfGFP fusion is expressed from its native genetic location, supports native secretion (Fig.…”
Section: Discussionmentioning
confidence: 99%
“…A prime candidate to establish the site of assembly for new injectisomes is the pilotin, a lipoprotein anchored in the inner leaflet of the outer membrane (OM). Pilotins are required for the proper integration and oligomerization of the secretin protein, which forms a large channel in the OM in the T3SS of animal pathogens, but also type II secretion systems (T2SS) and type IV pili (T4P) [15][16][17][18][19][20][21][22][23][24][25][26][27]. Since the secretin ring is one of the nucleation structures of the injectisome [6,28] and anchors the T3SS at its final position in the peptidoglycan mesh [29] (Fig.…”
Section: Introductionmentioning
confidence: 99%
“…The ϳ30-kb ysa locus contains a long operon with genes encoding the secretion apparatus, translocon proteins, and one effector protein. Adjacent to this operon, located on the opposite strand, are a few open reading frames encoding the recently identified YsaP pilotin protein (36) and other proteins that are likely part of the secretion apparatus. The ysrRST genes are just downstream of this region, and it was their proximity to the ysa genes that led us to investigate their role in ysa gene expression (22).…”
Section: Resultsmentioning
confidence: 99%