Identification of Zoophilic Dermatophytes Using MALDI-TOF Mass Spectrometry

Baumbach, Christina‐Marie; Müller, Stefanie H.; Reuschel, Maximilian; Uhrlaß, Silke; Nenoff, P.; Baums, Christoph Georg; Schrödl, Wieland

doi:10.3389/fcimb.2021.631681

Cited by 14 publications

(9 citation statements)

References 41 publications

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“…Zoophilic species naturally colonise animals, and their transfer to humans is not only possible but quite frequent. It usually occurs through reservoirs, which may be the animals themselves, their fur hairs, or objects with which they came into contact [ 8 ]. Zoophilic dermatophytes isolated from animals may be responsible for both symptomatic infections as well for asymptomatic colonisation, making the animals asymptomatic carriers which may become a source of the epidemic [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Laboratory Diagnosis and In Vitro Antifungal Susceptibility of Trichophyton quinckeanum from Human Zoonoses and Cats

et al. 2022

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The “One Health” concept increasingly demonstrates the global spread of pathogenic (also eukaryotic) microorganisms and their zoonotic potential. Dermatophytes can cause superficial mycoses in humans and animals. Furthermore, the number of transmissions from asymptomatic carriers to humans has been on the rise over the last few years. This study was focused on the detailed characterisation of clinical isolates of Trichophyton quinckeanum with epidemiological analyses and characterisation of their in vitro antifungal susceptibility patterns. The isolated dermatophytes were identified with a combination of conventional and molecular methods. In turn, their susceptibility in vitro was tested according to the Clinical and Laboratory Standards Institute (CLSI) M38 ed.3 protocol. A total of 36 strains were isolated, with 21 cases of T. quinckeanum zoonoses resulting from direct contact with symptomatic cats (58.3%). The other 15 strains (41.7%) were isolated simultaneously from healthy cats and their owners. All strains showed high susceptibility to allylamine, pyridinone, and phenyl morpholine derivatives but were resistant to fluconazole and ketoconazole. In conclusion, our study shows the frequency of zoonoses contracted from asymptomatic cats. Moreover, the antifungal susceptibility profiles indicate the serious risk posed to animal owners by resistant strains of T. quinckeanum, which are often responsible for recalcitrant-to-treatment cases.

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Section: Introductionmentioning

confidence: 99%

Laboratory Diagnosis and In Vitro Antifungal Susceptibility of Trichophyton quinckeanum from Human Zoonoses and Cats

et al. 2022

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“…At present, most microbiology laboratories still use Traditional morphological methods to identify filamentous fungi, but it is difficult and time-consuming to identify strains with no typical characteristics, which is not conducive to rapid and precise treatment. 20 , 21 There have been reports on the application of MALDI-TOF MS technical to identify filamentous fungi. Twenty-seven strains of filamentous fungi were used in this study.…”

Section: Discussionmentioning

confidence: 99%

“…conducive to rapid and precise treatment. 20,21 There have been reports on the application of MALDI-TOF MS technical to identify filamentous fungi. Twenty-seven strains of filamentous fungi were used in this study.…”

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confidence: 99%

Comparison of Autof Ms1000 and EXS3000 MALDI-TOF MS Platforms for Routine Identification of Microorganisms

Xiong¹,

Xu²,

Ni³

et al. 2023

IDR

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Matrix-assisted laser desorption-ionization-time of flight mass spectrometry (MALDI-TOF) has recently been widely used in clinical microbiology laboratories, with the advantages of being reliable, rapid, and cost-effective. Here, we reported the performance of two MALDI-TOF MS instruments, EXS3000 (Zybio, China) and Autof ms1000 (Autobio, China), which are commonly used in clinical microbiology field. Methods: A total of 209 common clinical common isolates, including 70 gram-negative bacteria strains, 58 gram-positive bacteria strains, 33 yeast strains, 15 anaerobic bacteria strains, and 33 mold strains, and 19 mycobacterial strains were tested. All strains were identified by EXS3000 (Zybio, China) and Autof ms1000 (Autobio, China). Sequence analysis of 16S rRNA or ITS regions was used to verify all strains. Results: Current study found that species-level discrimination was found to be 191 (91.39%) and 190 (90.91%) by EXS3000 and Autof ms1000, respectively. Genus-level discrimination was 205 (98.09%) by the EXS3000 and 205 (98.09%) by the Autof ms1000, respectively. The correct results at species level of the EXS3000 were 91.43% (64/70) for gram-negative bacteria, 93.1% (54/58) for gram-positive cocci, 93.94% (31/33) for yeast, 100% (15/15) for anaerobes and 81.82% (27/33) for filamentous fungi. The correct results at species level of the Autof ms1000 were 92.86% (65/70) for gram-negative bacteria, 91.38% (53/58) for gram-positive cocci, 93.94% (31/33) for yeast, 100% (15/15) for anaerobes and 78.79% (26/33) for filamentous fungi. Conclusion:Although the results show that the EXS3000 and Autof ms1000 systems are equally good choices in terms of analytical efficiency for routine procedures, the test result of EXS3000 is slightly better than Autof ms1000. It's worth mentioning that the target plate of the EXS 3000 instrument is reusable, but the target plate of the Autof ms1000 is disposable, making the EXS3000 more effective in reducing costs.

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“…However, each of these methods has its drawbacks. MALDI-TOF, in studies by Baumbach et al . (2021) , did not reveal the correct identification of the causative agents of dermatophytosis according to the databases provided by the software.…”

Section: Discussionmentioning

confidence: 99%

Comparative assessment of regulated methods and PCR in the diagnosis of trichophytosis in veterinary mycology

Umitzhanov,

Abdiramanova,

Abutalip

et al. 2023

Open Vet J

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Background: There is an increase in the incidence of human and animal infectious skin diseases of fungal aetiology in the world. The main source of infecting the population has become agricultural and stray animals. Aim: The objective of this study was to examine the morphophysiological and microbiological characteristics of pathogenic fungi belonging to the species Trichophyton verrucosum. This species is known to cause diseases in both humans and livestock in Kazakhstan. Additionally, the study aimed to assess the feasibility of using the polymerase chain reaction (PCR) method for detecting T. verrucosum. This assessment was conducted in comparison to the outcomes of conventional laboratory diagnostic tests commonly employed for trichophytosis. Methods: The research focused on analyzing 141 samples of pathological material obtained from calves in Almaty, Turkestan, and Kyzylorda regions. These calves exhibited clinical symptoms of skin disease. The study aimed to identify the causative agent using various techniques, including microscopic examination, microbiological methods involving the isolation of pure cultures, and polymerase chain reaction. Results: The detection of the causative agent of dermatophytosis using conventional methods was relatively low, 86% for the microscopic method and 79% for the microbiological method with the isolation of the culture of the pathogen. Extraction and detection of the genetic material of the causative agent of the disease for polymerase chain reaction was carried out according to the method developed by the authors. The effectiveness of the polymerase chain reaction (PCR) method was 97.9%, which is significantly higher (p<0.05) compared with the diagnostic effectiveness of conventional methods. The polymerase chain reaction method using specific primers identified the causative agent in 98% of cases, which significantly (p<0.05) exceeded the results obtained using conventional diagnostic methods. Accordingly, the PCR method had better sensitivity and specificity indicators. Conclusion: The conducted study allowed recommending the method of PCR diagnosis of dermatophytosis for fast and reliable confirmation of the diagnosis of dermatophytosis in humans and animals in Kazakhstan.

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Identification of Zoophilic Dermatophytes Using MALDI-TOF Mass Spectrometry

Cited by 14 publications

References 41 publications

Laboratory Diagnosis and In Vitro Antifungal Susceptibility of Trichophyton quinckeanum from Human Zoonoses and Cats

Laboratory Diagnosis and In Vitro Antifungal Susceptibility of Trichophyton quinckeanum from Human Zoonoses and Cats

Comparison of Autof Ms1000 and EXS3000 MALDI-TOF MS Platforms for Routine Identification of Microorganisms

Comparative assessment of regulated methods and PCR in the diagnosis of trichophytosis in veterinary mycology

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