Identification, purification and partial characterization of a 70 kDa inhibitor protein of Na+/K+-ATPase from cytosol of pulmonary artery smooth muscle

Dey, Kuntal; Chakraborti, Tapati; Roy, Subhasish; Ghosh, Biswarup; Kar, Pulak; Chakraborti, Sajal

doi:10.1016/j.lfs.2010.02.002

Cited by 5 publications

(5 citation statements)

References 46 publications

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“…The maximal ouabain-binding capacity, that is, the number of Na + /K + -ATPase molecules, of normal human skeletal muscle (360 + 70 pmol/g wet wt. ; n = 5) agrees with earlier data (Nagel et al, 1987;Dey et al, 2010).…”

Section: Groupsupporting

confidence: 92%

Effect of Salvia miltiorrhiza extract on blood lipid, hydroxyproline levels, skeletal muscle Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities in swimming exercise rats

Yun¹,

Tao²

2011

Afr. J. Pharm. Pharmacol.

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The purpose of the present study was to examine the effects of aqueous extract of Salvia miltiorrhiza on plasma lipid and hydroxyproline levels and skeletal muscle Na + -K + -ATPase and Ca 2+ -Mg 2+ -ATPase activities in swimming exercise rats. Adult Sprague-Dawley male rats, 3 months of age, were assigned randomly to a sedentary control (n = 10) or exercise (n = 10) group or SME-treatent groups (exercise + SME) (n = 10 in each group). Ten animals in sedentary control group did not receive any exercise. Animals in exercise group received exercise. Animals in SME-treatent groups (exercise + SME) received exercise orally given with S. miltiorrhiza extract (50, 100 or 150 mg/kg body weight), respectively. Result showed that aqueous extract of S. miltiorrhiza can decrease blood total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-c) and increase high-density lipoprotein cholesterol (HDL-c) levels, enhance hydroxyproline levels in bone, skeletal muscle, heart and lung, and skeletal muscle Na + -K + -ATPase and Ca 2+ -Mg 2+ -ATPase activities in swimming exercise rats.

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Section: Groupsupporting

confidence: 92%

Effect of Salvia miltiorrhiza extract on blood lipid, hydroxyproline levels, skeletal muscle Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities in swimming exercise rats

Yun¹,

Tao²

2011

Afr. J. Pharm. Pharmacol.

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“…Recently, we have reported a 70 kDa inhibitor protein of NKA in bovine pulmonary artery smooth muscle (BPASM) that inhibits the pump upon binding to its E1 site. We have also observed that the inhibitor showed higher affinity towards the a 2 isoform of NKA than the a 1 isoform [16].…”

Section: Introductionmentioning

confidence: 51%

“…In view of our recent report about the presence of a 70 kDa inhibitor of NKA [16], we also determined its role in regulating the enzyme activity upon stimulation of the cells with U46619.…”

Section: Introductionmentioning

confidence: 99%

“…Since BPASMCs contain both the a 1 and a 2 isoforms of NKA [11], therefore, one of our aims was to investigate the isoform specific regulation of NKA by oxidants and to explore the underlying mechanisms. In view of our recent report about the presence of a 70 kDa inhibitor of NKA [16], we also determined its role in regulating the enzyme activity upon stimulation of the cells with U46619.…”

Section: Introductionmentioning

confidence: 99%

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Role of phospholemman and the 70 kDa inhibitor protein in regulating Na⁺/K⁺ ATPase activity in pulmonary artery smooth muscle cells under U46619 stimulation

Dey¹,

Rahaman²,

Chakraborti³

et al. 2013

FEBS Letters

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a b s t r a c tTreatment of bovine pulmonary smooth muscle cells with U46619 inhibited the Na + /K + ATPase activity in two parallel pathways: one of which is mediated via glutathionylation of the pump and the other by augmenting the inhibitory activity of the 70 kDa inhibitor protein of Na + /K + ATPase. Although phospholemman deglutathionylates the pump leading to its activation, the inhibitor is responsible for irreversible inhibition of Na + /K + ATPase in an isoform specific manner during treatment of the cells with U46619.

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“…Circulating inhibitors of sodium-potassium adenosine triphosphatase (Na-K-ATPase) have been shown to be of possible pathogenetic importance in the mechanism of essential hypertension ( 1 – 3 ). Although previous studies have demonstrated the presence of both high-molecular weight (HMW), ranging from 11 to 70 kDa ( 4 – 8 ) and low-molecular weight (LMW) either natriuretic or Na-K-ATPase inhibitors, no previous attempts had been made to ascertain whether HMW or LMW forms predominate in hypertension. This review summarizes the steps taken by our laboratory to first identify the HMW form, and then split off the final LMW form of the hormone.…”

Section: Introductionmentioning

confidence: 99%

Evidence for a 12â€‰kDa â€œCarrier Proteinâ€ for Natriuretic Hormone

Gonick

2014

Front. Endocrinol.

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The search for the elusive Na-K-ATPase-inhibiting natriuretic hormone continues. In this review, evidence is presented that isolating the carrier protein for natriuretic hormone from hypertensive plasma is a necessary first step before splitting off the final hormone. The carrier protein has a molecular weight of 12 kDa while the final hormone has a molecular weight of 408 Da. Both compounds inhibit Na-K-ATPase but the compound containing the carrier protein predominates. The question has been raised as to whether the carrier protein is in actuality proANF, a 17 kDa protein that can be split between a 14 kDa protein (the presumptive proANF) and the 3 kDa ANF.

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Identification, purification and partial characterization of a 70 kDa inhibitor protein of Na+/K+-ATPase from cytosol of pulmonary artery smooth muscle

Cited by 5 publications

References 46 publications

Effect of Salvia miltiorrhiza extract on blood lipid, hydroxyproline levels, skeletal muscle Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities in swimming exercise rats

Effect of Salvia miltiorrhiza extract on blood lipid, hydroxyproline levels, skeletal muscle Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities in swimming exercise rats

Role of phospholemman and the 70 kDa inhibitor protein in regulating Na⁺/K⁺ ATPase activity in pulmonary artery smooth muscle cells under U46619 stimulation

Evidence for a 12â€‰kDa â€œCarrier Proteinâ€ for Natriuretic Hormone

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Identification, purification and partial characterization of a 70 kDa inhibitor protein of Na+/K+-ATPase from cytosol of pulmonary artery smooth muscle

Cited by 5 publications

References 46 publications

Effect of Salvia miltiorrhiza extract on blood lipid, hydroxyproline levels, skeletal muscle Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities in swimming exercise rats

Effect of Salvia miltiorrhiza extract on blood lipid, hydroxyproline levels, skeletal muscle Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities in swimming exercise rats

Role of phospholemman and the 70 kDa inhibitor protein in regulating Na+/K+ ATPase activity in pulmonary artery smooth muscle cells under U46619 stimulation

Evidence for a 12â€‰kDa â€œCarrier Proteinâ€ for Natriuretic Hormone

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Role of phospholemman and the 70 kDa inhibitor protein in regulating Na⁺/K⁺ ATPase activity in pulmonary artery smooth muscle cells under U46619 stimulation

Evidence for a 12â€‰kDa â€œCarrier Proteinâ€ for Natriuretic Hormone