Identification, tissue distribution and evaluation of brain neuropeptide Y gene expression in the Brazilian flounder Paralichthys orbignyanus

Campos, Vinícius Farias; Collares, Tiago; Deschamps, João Carlos; Seixas, Fabiana Kömmling; Dellagostin, Odir A.; Lanes, Carlos Frederico Ceccon; Sandrini, Juliana Zomer; Marins, Luís Fernando; Okamoto, Marcelo Hideo; Sampaio, Luís André; Robaldo, Ricardo Berteaux

doi:10.1007/s12038-010-0046-y

Cited by 37 publications

(19 citation statements)

References 34 publications

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“…In zebrafish, after 7 days of food deprivation, the expression of npy mRNA was significantly increased in the hypothalamus (Yokobori et al, ). Campos et al () also found that the npy mRNA expression in the brain was higher than in fed fish after 2 weeks of fasting in Brazilian flounder. After refeeding, we found that the expression level of npy mRNA in the refed group was significantly lower than in the fed group ( p < 0.01).…”

Section: Discussionsupporting

confidence: 83%

Cloning, expression profiling, and effects of fasting status on neuropeptide Y in Schizothorax davidi

et al. 2019

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To better comprehend the mechanism that neuropeptide Y (npy) regulates feeding in Schizothorax davidi, we cloned and identified the full‐length cDNA sequence of the npy gene in this species using RACE technology. Subsequently, we explored the npy mRNA distribution in 18 tissues and investigated the expression of npy mRNA at postprandial and fasting stages. We found that the npy full‐length cDNA sequence is 803 bp. Moreover, npy mRNAs extensively expressed in all detected tissues, with the highest expression in hypothalamus. In postprandial study, the expression of npy mRNA in the hypothalamus was significantly decreased after eating (p < 0.01). In addition, the expression of the npy gene was significantly increased on the fifth day after fasting (p < 0.05). However, after refeeding, the expression of the npy gene was decreased significantly on days 9, 11, and 14 (p < 0.01). Our research suggest that npy may have an orexigenic role in S. davidi. Practical applications S. davidi, a coldwater fish native to China, has high economic value, and it has gained great popularity. To date, there is still no large‐scale breeding of S. davidi in China. How to strengthen the production performance of S. davidi is a hot research area. Neuropeptide Y (NPY), a 36‐amino‐acid single‐chain polypeptide, is one of the main appetite regulation factors. However, to date, no studies have reported on the biological function of npy in the feeding of S. davidi. In our study, we revealed that the trend of hypothalamic npy expression during the postprandial and fasting stages. The results suggested that npy might be an appetite‐promoting factor in this species. Overall, we provide the theoretical basis for how to strengthen the production performance of S. davidi through appetite regulation.

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Section: Discussionsupporting

confidence: 83%

Cloning, expression profiling, and effects of fasting status on neuropeptide Y in Schizothorax davidi

et al. 2019

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“…Total RNA extraction, cDNA synthesis and real-time PCR (qPCR) were carried out as previously described (Campos et al, 2010). Briefly, RNA samples were isolated using TRIzol Reagent (Invitrogen) and samples were DNase-treated with a DNA-free kit (Ambion, USA) following the manufacturer's protocol.…”

Section: Gene Expression Evaluation By Real-time Pcrmentioning

confidence: 99%

Substituted diaryl diselenides: Cytotoxic and apoptotic effect in human colon adenocarcinoma cells

et al. 2012

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“…Blood was frozen and stored in liquid nitrogen until analysis. Total RNA extraction and cDNA synthesis was carried out as previously described (Campos et al 2010a). Briefly, RNA samples were isolated using TRIzol Reagent (Invitrogen, Carlsbad, USA) and samples were DNase-treated with a DNA-free kit (Ambion, USA) following the manufacturer's protocol.…”

Section: Transgene Transmission Of Exogenous Dna Treatedmentioning

confidence: 99%

Transgene transmission in chickens by sperm-mediated gene transfer after seminal plasma removal and exogenous DNA treated with dimethylsulfoxide or N,N-dimethylacetamide

et al. 2011

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Transgenic animals have been successfully produced by mass gene transfer techniques such as sperm-mediated gene transfer (SMGT). The aim of this work was to demonstrate transgene transmission by SMGT in chickens using dimethylsulfoxide (DMSO) or N,N-dimethylacetamide (DMAc) as transfectants after seminal plasma removal to prevent DNase activity. Sperm samples were prepared by repetitive washes, and after each wash sperm motility, seminal plasma proteins, exogenous DNA integrity and its uptake by spermatozoa were evaluated. Laying hens were inseminated using spermatozoa transfected with pEGFP-N1 vector in the presence of DMSO or DMAc. Transgene transmission in newborn chicks was evaluated by in vivo enhanced green fluorescent protein (EGFP) expression, RT-PCR and PCR analysis. DNA internalization was limited to sperm samples washed twice. The presence of DMSO or DMAc during transfection had no effect on fertilization or hatching rates. PCR analysis detected the presence of EGFP DNA in 38% of newborn chicks from the DMSO group and 19% from the DMAc group. EGFP mRNA was detected in 21% of newborn chicks from the DMSO group, as against 8.5% from the DMAc group. However, in vivo expression of EGFP was only observed in a single animal from the DMSO group. Our data revealed that the plasmid DNA-DMSO combination coupled with sperm washes can be an efficient method for transfection in chickens.

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Identification, tissue distribution and evaluation of brain neuropeptide Y gene expression in the Brazilian flounder Paralichthys orbignyanus

Cited by 37 publications

References 34 publications

Cloning, expression profiling, and effects of fasting status on neuropeptide Y in Schizothorax davidi

Cloning, expression profiling, and effects of fasting status on neuropeptide Y in Schizothorax davidi

Substituted diaryl diselenides: Cytotoxic and apoptotic effect in human colon adenocarcinoma cells

Transgene transmission in chickens by sperm-mediated gene transfer after seminal plasma removal and exogenous DNA treated with dimethylsulfoxide or N,N-dimethylacetamide

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