Identification variétale des orges par électrophorèse. Description de 280 variétés

Lallemand, J.; Briand, F.

doi:10.1051/agro:19900602

Cited by 7 publications

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“…Hordein patterns ere classi ed using the Lallemand Briand system ith modi cations Lallemand and Briand, 1990 . Hordein formulas ere constructed according to Dimova 2011 .…”

Section: Methodsmentioning

confidence: 99%

Нordein polymorphism between spring barley cultivars by SDS-PAGE electrophoresis

Neykov¹,

Doneva²

2017

AST

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Abstract. In this study reserve endosperm proteins, hordeins of seven spring barley cultivars with different origin: Zernogradskii (Russia), Bodega, Fink, Scarlett and Barke (Germany), Josefin and Astoria (France) were fractionated and characterized by SDS-PAGE electrophoresis. On the basis of the obtained spectra 19 bands (D + C + B) with different relative electrophoretic mobility and intensity were identified. The electrophoresis profiles of the groups D-, C-and Bhordein are designated as separate types (models) using the index corresponding to hordein blocks. We have established one profile type for D-hordein (D1), two-for C-hordein (C1, C2), and five -for B-hordein (B1, B2, B3, B4, B5). Based on these results hordein formulas (configurations) of accessions are constructed, which enable the expression of specific varietal characteristics and prove the existence of the inter allelic variation (hordein polymorphism) due to the presence or absence of protein components and their different electrophoretic mobility in the profiles of D-, C-and B-hordein.Keywords: spring barley, storage proteins, hordein types, intervarietal polymorphism, SDS-PAGE AGRICULTURAL SCIENCE AND TECHNOLOGY, VOL. 9, No 4, pp 273 -276, 2017 DOI: 10.15547/ast.2017 IntroductionThe use of biochemical markers of genetic control of useful traits is increasingly used in the breeding of barley (Jones, 1982; Hauser et al., 1982;Stoyanova and Popova, 2002). As a result of long research it was found, that electrophoretic spectra of reserve proteins in barley -hordeins -remain unchanged under the influence of environmental conditions (Konarev, 1983(Konarev, , 1983a. Studying the genetic nature of the traits in barley varieties on the basis of polymorphism of reserve proteins is a guarantee for a successful breeding programme (Konarev et al., 1986). The determination of reserve proteins is widely used in studies of plant populations, since variability in their characteristics is genetically determined. They are characterized by a high level of polymorphism and stability.Barley storage proteins -hordeins -have been divided into three groups on the basis of their electrophoretical mobility and amino acid composition (Shewry and Milfin, 1985). D-hordeins have the highest molecular weight (105 kD); they are characterized by a high amino acid (glutamine, glicine and proline) content (Shewry and Tatham, 1990). Synthesis of these hordeins is encoded by the Hor 3 locus located on the long arm of chromosome 1H(5) (Kreis et al., 1984). C-hordeins (50-80 kD), rich in glutamine, phenylalanine and proline, and the major B-hordeins (36-45 kD), rich in glutamine, are encoded by the Hor 1 and Hor 2 loci, respectively, both located on the short arm of chromosome 1H(5) (Shewry and Milfin, 1985). The advantages of hordeins for studying the genetic diversity in barley have been described by many authors (Pomortsev et. al., 2002;Vyhnanek et al., 2003). The barley storage protein hordein is characterized by a high level of polymorphism (Dimova, 2011;Mihova et al., 2...

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“…Hordein patterns ere classi ed using the Lallemand Briand system ith modi cations Lallemand and Briand, 1990 . Hordein formulas ere constructed according to Dimova 2011 .…”

Section: Methodsmentioning

confidence: 99%

Нordein polymorphism between spring barley cultivars by SDS-PAGE electrophoresis

Neykov¹,

Doneva²

2017

AST

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“…Hordein polymorphism was studied by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) according to Lallemand and Briand (1990). The stacking gel layers contained 4% (w/v) acrylamide and 0.11% (w/v) N,N0methylenebisacrylamide (BIS); the separating gels contained 12% (w/v) acrylamide and 0.024% (w/v) BIS.…”

Section: Hordein Electrophoresismentioning

confidence: 99%

“…Brilliant Blue R250 for 45 minimums at 45 _C according to Lallemand and Briand (1990). After staining, gels were stored in 10% acetic acid.…”

Section: Staining and Storage Of Gelsmentioning

confidence: 99%

Evaluation of genetic diversity of wild-type barley (Hordeum vulgare L. sub sp. Spontaneum) based on storage proteins polymorphism

2014

Int. J. Biosci.

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Today, most scientists have accepted that cultivated barley was originated from wild-type barley, Hordeum spontaneum. By the way, this is unclear whether domestication processes just occurred in Fertile Crescent or simultaneously in different areas all over the world. Additionally, there are disagreements in relation to center or main diversity centers of this plant. In the present study, in order to evaluate genetic and geographic diversity and also determining of barley populations phylogenic relations, 266 wild-type, H. spantaneum, from areas located in or out of Fertile Crescent and also 44 samples of cultivated barley from different world areas were studied. Barley samples storage proteins were analyzed by SDS-PAGE method and were assessed in three hordein groups including D, C and B. Genetic parameters, such as patterns number and genetic diversity index showed that by getting away from Fertile Crescent, barley genetic diversity was reduced, However, North Africa and East Asia could be considered as a secondary centers of diversity of the plant. The study of genetic distances showed that wild barley genotypes of Iraq and Turkey could be the nearest populations to modern cultivated barley samples. Based on these results, Fertile Crescent especially Iran, Iraq, South East of Turkey and Jordan could be main centers of diversity of wild barley H. Spontaneum.

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“…Stegemann & Loeschcke, 1976;Stegemann & Schnick, 1985;Gardiner & Forde, 1987;Cooke, 1984Cooke, , 1989Cooke, , 1992Van Dreven et al, 1990;Lallemand & Briand, 1990). However, research on electrophoresis in bulb crops is rather limited.…”

Section: Introductionmentioning

confidence: 99%

Identification of tulip cultivars based on polymorphism in esterase isozymes from bulb scales

1993

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Identification variétale des orges par électrophorèse. Description de 280 variétés

Cited by 7 publications

References 0 publications

Нordein polymorphism between spring barley cultivars by SDS-PAGE electrophoresis

Нordein polymorphism between spring barley cultivars by SDS-PAGE electrophoresis

Evaluation of genetic diversity of wild-type barley (Hordeum vulgare L. sub sp. Spontaneum) based on storage proteins polymorphism

Identification of tulip cultivars based on polymorphism in esterase isozymes from bulb scales

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