Identifikasi kutukebul (Hemiptera: Aleyrodidae) dari beberapa tanaman inang dan perkembangan populasinya

Yuliani, Yuliani; Hidayat, Purnama; Sartiami, Dewi

doi:10.5994/jei.3.1.41

Cited by 3 publications

(1 citation statement)

References 4 publications

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“…Dengan bantuan mikroskop, puparium dilepaskan secara perlahan-lahan dari jaringan daun menggunakan jarum bertangkai yang tipis dan dimasukkan ke dalam tabung berisi alkohol 70%. Selanjutnya puparium disiapkan untuk dibuat preparat mengikuti prosedur (Yuliani et al 2006). Puparium dipindahkan ke gelas arloji yang berisi alkohol 80% selama 10 menit, lalu dipindahkan secara hati-hati ke dalam tabung reaksi yang berisi larutan KOH 10%, kemudian dipanaskan selama 5-10 menit (sampai puparium menjadi transparan).…”

Section: Identifikasi Kutukebulunclassified

Distribution of Yellow Curly Leaf Disease in Chili Plantations in Southeast Sulawesi and Identification of the Causes

Taufik¹,

Hs²,

Syair³

et al. 2023

J Fitopatol Indones

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Penyakit daun keriting kuning pada pertanaman cabai di Sulawesi Tenggara telah dilaporkan sejak tahun 2018, yaitu di Kabupaten Kolaka Timur dan Kota Kendari. Gejala penyakit di lapangan semakin meluas seiring dengan perluasan penanaman cabai. Penelitian bertujuan menghitung kembali insidensi penyakit daun keriting kuning pada pertanaman cabai di Sulawesi Tenggara, mengidentifikasi serangga yang berasosiasi dengan tanaman cabai, dan mengidentifikasi penyebab penyakitnya. Pengamatan insidensi penyakit dilakukan di pertanaman cabai yang berada di Kabupaten Bombana, Konawe Selatan, Konawe, Kendari, Kolaka Timur, Kolaka, dan Kolaka Utara. Identifikasi kutukebul dilakukan berdasarkan karakter morfologi. Deteksi dan identifikasi begomovirus menggunakan metode polymerase chain reaction, yang dilanjutkan dengan analisis sikuensing. Rata-rata insidensi penyakit daun keriting kuning di tujuh kabupaten ialah 36%–90%. Spesies kutukebul yang ditemukan adalah Aleurotrachelus trachoides. Fragmen DNA spesifik begomovirus berukuran 580 pb berhasil diamplifikasi dari sampel tanaman cabai asal tujuh kabupaten di Sulawesi Tenggara. Analisis sikuen mengonfirmasi infeksi Pepper yellow leaf curl Indonesia virus pada pertanaman cabai di Kabupaten Kolaka, Kolaka Utara, Bombana, Konawe, dan Konawe Selatan.

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Section: Identifikasi Kutukebulunclassified

Distribution of Yellow Curly Leaf Disease in Chili Plantations in Southeast Sulawesi and Identification of the Causes

Taufik¹,

Hs²,

Syair³

et al. 2023

J Fitopatol Indones

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Viral complexity of tomatoes yellow mosaic and leaf curl diseases in lowland and highland areas

Hermanto,

Murti,

Hartono

et al. 2024

Journal of Phytopathology

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Yellow mosaic and leaf curl diseases caused by whitefly‐transmitted begomo virus genera are important problems in tomato (Solanum lycopersicon L.) production in Indonesia, particularly on Java Island. The aims of this survey are to assess the percentages of disease incidence (DI) and disease severity index (DSI), evaluate the spread and complexity of begomovirus and crinivirus infections responsible for yellow mosaic and leaf curl diseases in tomato plants, and identify the species of whitefly insect vectors and their cryptic biotypes in tomato production centers located on Java Island, Indonesia. A total of 43 locations in East, Central, and West Java were surveyed during the dry season from July 2022 to September 2022. Disease incidence and severity were determined by observing 200 samples of tomato plants from five points at each location, with 40 plants per point of land. A total of 116 whitefly samples in microtubes and 215 symptomatic leaf samples were collected. The genus begomovirus and crinivirus infecting tomato were identified by PCR and RT‐PCR using genus‐specific primers. Similarly, whitefly species and their cryptic biotypes were identified by PCR using MCoI gene‐specific primers. Results showed that DI and DSI remarkably differed between the lowlands (66%–89%) and highlands (33%–62%) in the provinces of Central Java and West Java. Furthermore, PCR analysis of 215 infected tomato showing the symptoms of yellow mosaic and curly leaf revealed that three begomoviruses, one crinivirus and their mixed combination both begomoviruses, one crinivirus has been detected in all the samples. Similarly, the whitefly insect vector Bemisia tabaci biotype Q was identified in all areas, whereas Trialeurodes vaporariorum was found only in the East Java and Java highlands, respectively.

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Virulence of Five Isolates of Indigenous Beauveria Bassiana Against Eggs and Nymphs of Bemisia Tabaci Gennadius (Hemiptera: Aleyrodidae)

2021

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This research aims to study the virulence of five isolates of Beauveria bassiana to eggs and nymphs of Bemisia tabaci on tomato. In the experiment eggs and second instar nymphs of B. Tabaci were used. Five isolates of the fungus, i.e., WS, TD312, PD114, PA221, PB211, were tested. Conidial concentration of B. bassiana used were 108 conidia/ml. Experimental parameters included mortality of eggs and nymph and percentage of adult emergence. The results showed that all B. bassiana isolates tested were able to kill B. tabaci eggs but with very low mortality (2-19%). Mortality of second instar B. tabaci nymphs was dependent on the fungal isolates. Isolate WS had the highest virulence, which caused 70% mortality of 2nd instar nymphs, with a LT50 of 4.87 days. Nymphs of B. tabaci were highly susceptible to B. bassiana infection compared with eggs. B. bassiana applicated to nymphs of B. tabaci can decrease the percentage of adult emergence.

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Identifikasi kutukebul (Hemiptera: Aleyrodidae) dari beberapa tanaman inang dan perkembangan populasinya

Cited by 3 publications

References 4 publications

Distribution of Yellow Curly Leaf Disease in Chili Plantations in Southeast Sulawesi and Identification of the Causes

Distribution of Yellow Curly Leaf Disease in Chili Plantations in Southeast Sulawesi and Identification of the Causes

Viral complexity of tomatoes yellow mosaic and leaf curl diseases in lowland and highland areas

Virulence of Five Isolates of Indigenous Beauveria Bassiana Against Eggs and Nymphs of Bemisia Tabaci Gennadius (Hemiptera: Aleyrodidae)

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