Identifikasi Senyawa Aktif Ekstrak Buah Mengkudu (Morinda citrifolia L.) dan Potensinya sebagai Inhibitor Karies Gigi

Abstract

Dental caries is a disease that many people experience, which is formed by the buildup of plaque on the teeth and the presence of bacteria. The bacteria known to be involved in the formation of dental caries is Streptococcus mutans. Dental caries used to be treated using antibiotics. However, it is known that many antibiotics are no longer effective against this bacteria hence it is necessary to look for another alternative. One alternative choice as an antibacterial is Noni fruit. This study aims to identify the bioactive compounds of noni fruit (Morinda citrifolia L) and to know the mechanism of action in inhibiting the growth of Streptococcus mutans. Noni fruit was macerated using 96% ethanol, then fractionated using n-hexane and ethyl acetate. Antibacterial activity test was done using agar difusion method, and identification of bioactive compounds was conducted using GCMS. The results showed that ethyl acetate fraction has the highest inhibitory activity on the growth of Streptococcus mutans with inhibition zone of ±17 mm and MIC (minimum inhibitor concentration) value of 10%. The inhibition mechanism is asumed to be done by making holes in cell membranes, which can be seen from the high concentration of proteins and nucleic acids coming out of cells after treatment that in turn means that cell leakage has occurred. The results of this study indicate that noni fruit (Morinda citrifolia L) contain bioactive quinolone compounds that can be a new source for treating dental caries

THE EFFECT OF PANDAN WANGI ROOTS ETHANOL EXTRACT (Pandanus amaryllifolius Roxb.) ON THE DEGREE OF PLASMODIUM BERGHEI PARASITEMIA IN VIVO

“…9 Various herbal plants have been studied to prove the effectiveness of alkaloid compounds as antimalarials, such as papaya leaf extract (Carica papaya), guava leaf extract (Psidium guajava), 17 merkubung leaf extract (Macaranga gigantea), 18 gempol leaf extract (Nauclea orientalis L.), 19 and noni fruit extract (Morinda citrifolia L.). 20 Based on the previous studies outlined above, it is evident that many herbal plants have the potential as antimalarials.…”

Phytochemistry and antifatigue activities of Carica papaya leaf from geothermal, coastal and urban areas, Indonesia

“…9,12,15-octadecatrienoic acid is grouped into fatty acids and esters which are useful as the main energy provider during cell growth and this compound plays a role in anti-melanogenic, antioxidant, anti-fungal and anti-inflammatory activities [37,38]. Squalene is a compound found in papaya and moringa leaves which functions as an antioxidant [23,39]. Vitamin E is known to have antioxidant effects so that it can protect muscle tissue from various damages caused by ROS and increase glucose absorption as an energy source from carbohydrate metabolism, optimize pancreatic function with effective insulin work, and the adipogenesis process [40,41].…”

Perbandingan Aktivitas Antibakteri Ekstrak Bunga dan Daun Beluntas (Pluchea indica (L.) less.) Terhadap Bakteri Staphylococcus aureus

“…Kemudian ditambahkan 10 mL air panas kemudian didinginkan dan dikocok vertikal selama 10 detik.Adanya pembentukan busa setinggi 1-10 cm yang stabil selama tidak kurang dari 10 menit menunjukkan adanya kandungan senyawa saponin.Penambahan 1 tetes HCl 2N maka busa tidak hilang [14] . e) Uji identifikasi steroid dan triterpenoid Ditambahkan 10 tetes CH 3 COOH glasial dan 2 tetes asam sulfat dalam ekstrak.Selanjutnya dikocok dan tunggu beberapa menit.Adanya senyawa steroid ditunjukkan dengan terbentuknya warna hijau kebiruan sedangkan untuk triterpenoid menunjukkan perubahan warna merah atau ungu [13] . f) Uji identifikasi Tanin Sebanyak 1,5 gram ekstrak dilarukan dalam 5 mL aquadest.…”