Identifikasi Uji Kemampuan Hidrolisis Lemak Dan Penentuan Indeks Zona Bening Asam Laktat Pada Bakteri Dalam Wadi Makanan Traditional Kalimantan Tengah

Rizky, Mirza Yanuar; Fitri, Rizka Diah; Hastuti, Utami Sri; Prabaningtyas, Sitoresmi

doi:10.35580/bionature.v18i2.6137

Cited by 4 publications

(4 citation statements)

References 11 publications

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“…Media NA akan ditambahkan lemak berupa minyak zaitun yang befungsi untuk substrat yang akan dihidrolisis oleh bakteri lipolitik. Isolat akan bersifat lipolitik jika menghasilkan zona bening disekitar koloni lipolitik (Astuti, 2017).…”

Section: Pengukuran Aktivitas Indeks Lipolitikunclassified

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Untitled

2023

BIOEDUSAINS

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Penelitian ini bertujuan untuk mengetahui kemampuan bakteri lipolitik dalam mendegradasi lipid dengan memperoleh isolat-isolat bakteri lipolitik pada limbah cair industri kelapa sawit. Metode penelitian yang digunakan adalah metode deskripsi dengan teknik isolasi bakteri menggunakan media NA(Nutrient Agar) dan media selektif agar lipolitik, dengan menguji kemampuan aktivitas indeks lipolitik. Hasil penelitian menunjukkan terdapat 10 isolat bakteri lipolitik dengan karakteristik morfologi dan biokimia menunjukkan tiga genus bakteri yaitu, genus Bacillus, Pseudomonas dan Klebsiella. Data analisa memperlihatkan bahwa 10 isolat bakteri lipolitik memiliki potensi dalam mendegradasi lipid, nilai indeks aktivitas lipolitik isolat terbesar pada isolat BL 1 sebesar 0,48 mm dan indeks lipolitik terendah dengan isolat BL 9 sebesar 0,16 mm. Kesimpulanya, dengan melakukan isolasi dan mengidentifikasi bakteri lipolitik pada limbah cair kelapa sawit merupakan salah satu cara untuk mengurangi pencemaran lingkungan yang merusak ekosistem yang ada dilingkungan.

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Section: Pengukuran Aktivitas Indeks Lipolitikunclassified

“…Zona bening dipengaruhi oleh media dan kondisi lingkungan. Menurut (Astuti, 2017) mengatakan bahwa menurunya kadar lemak terjadi karena lemak terhidrolisis menjadi gliserol dan asam lemak bebas. Asam lemak mudah mengalami kerusakan sehingga kadar lemak mengalami penurunan.…”

Section: Pengukuran Aktivitas Indeks Lipolitikunclassified

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2023

BIOEDUSAINS

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“…The isolates were then incubated at 30 o C for 48 hours. If a red color is formed at the bottom of the colony, then it is positive for fat hydrolysis (Yanuar et al 2017).…”

Section: Fat Hydrolysis Testmentioning

confidence: 99%

Characterization and molecular identification of bacteria from mackerel bekasam in Sorong City, Southwest Papua Province, Indonesia

SUKMAWATI,

RATNA,

SIPRIYADI

et al. 2023

Biodiversitas

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Abstract. Sukmawati S, Ratna R, Sipriyadi, Yunita M. 2023. Characterization and molecular identification of bacteria from mackerel bekasam in Sorong City, Southwest Papua Province, Indonesia. Biodiversitas 24: 4967-4977. Bekasam is traditional food type produced by traditional fermented fish. Microbes that grow through fermentation play an important role in forming the product’s aroma, texture, and overall quality. The study aimed to determine the biochemical characteristic of bacteria from mackerel (Scomberomorus sp.) bekasam in Sorong City and identify bacteria at the molecular level. This research was a descriptive study, which described the results of the characterization of bacteria from fermented mackerel fish and the results of molecular identification to the species level through the PCR (Polymerase Chain Reaction) technique. Then, the DNA sequences were further analyzed using the agarose gel electrophoretic separation method to visualize the bacterial DNA profile. The biochemical characterization of bacterial isolates from mackerel showed that all isolates were negative indole, and eight isolates were positive in reducing nitrate. In comparison, four isolates were negative in reducing nitrate, then all isolates had proteolytic activity except the FST 3.1 and FST 3.2 isolates. Eleven isolates were positive in hydrolyzing fat, and one isolate could not hydrolyze fat. According to the DNA patterns seen in electrophoresis and alignment of the 16 sRNA gene sequences, several types of bacteria had been identified as Bacillus paramycoides strain 2883 FST 1.1, Bacillus paramycoides strain 3665 FST 2.1, Bacillus mobilis strain ICA-144 FST 3.1, Bacillus cereus strain ATCC 14579 FNT 1.1, Bacillus mobilis strain ICA-144 FNT 2.1, and Bacillus cereus strain ATCC 14579 FNT 3.1.

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“…Kemudian media yang steril dipindahkan pada waterbath hingga digunakan (Simamora & Sukmawati, 2020) yang dimodifikasi. Cara menghitung indeks hidrolisis lemak yaitu (Rizky et al, 2017) Indeks hidrolisis lemak = diameter zona bening bakteri/diameter koloni bakteri.…”

Section: Pengukuran Aktivitas Bakteri Lipolitikunclassified

Studi Komunitas Bakteri Hidrolitik Saluran Pencernaan Ikan Nilem (Osteochilus vittatus) yang Dibudidayakan Di Kabupaten Banyumas

Listiowati,

Ekasanti,

Nugrayani

et al. 2022

Akuakultur

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Hydrolytic bacteria are bacteria that play an important role in helping the digestive process of fish. This study aims to determine the hydrolytic activity of bacteria in the digestive tract of Nilem fish seen from amylolytic, proteolytic, lipolytic and cellulitic indices. The method used is exploratory and data analysis using parametric and non-parametric to explain the research data. Exploration carried out in this study included the total number of bacteria, proportion of hydrolytic bacteria, activity index of hydrolytic bacteria and molecular identification of bacteria with the best activity index. Fish were taken at random from fish farming ponds in Beji and Singasari with three fish each. The number of bacteria found an increasing pattern to the posterior intestine of fish from Beji and a pattern that varied between parts of the intestine of fish from Singasari. Hydrolytic bacteria were isolated from each part of the intestine from both locations which showed a decreasing pattern in each type of hydrolytic and intestinal tracts of fish from Singasari and varied patterns in the intestines of fish from Beji. The hydrolytic activity index showed a stable average value in each part of the intestine and the sampling location. Based on the best activity index, seven isolates were identified from three different genera, namely 1) Bacillus subtilis, 2) Enterobacter mori, Enterobacter cloacae, and 3) Aeromonas hydrophila (3), Aeromonas veronii. The bacteria obtained are classified as non-pathogenic bacteria that have potential in the field of aquaculture and normal pathogenic bacteria in the fish intestine

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Identifikasi Uji Kemampuan Hidrolisis Lemak Dan Penentuan Indeks Zona Bening Asam Laktat Pada Bakteri Dalam Wadi Makanan Traditional Kalimantan Tengah

Cited by 4 publications

References 11 publications

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Characterization and molecular identification of bacteria from mackerel bekasam in Sorong City, Southwest Papua Province, Indonesia

Studi Komunitas Bakteri Hidrolitik Saluran Pencernaan Ikan Nilem (Osteochilus vittatus) yang Dibudidayakan Di Kabupaten Banyumas

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