Identifizierung verschiedener Miscanthus‐Sorten mittels Isoenzymen

Abstract

Identification of Miscanthus Varieties by their Isozymes With the aim of testing the possibility of identifying Miscanthus varieties, zymograms of 13 isozymes from 65 plants of M. smensis, M.×giganteus and M. sacchariflorus were analysed using starch‐gel electrophoresis. The zymograms of root, rhizome, and leaf tissues were compared. After similarity between the zymograms of the different types of tissues had been established, the tissue of young leaves was used for analysis. The zymograms of the different isozymes are variable in most cases and show as many as nine patterns. The large variation between the varieties allows an identification of each of the varieties analysed. About half of the plants could be attributed to M.×giganteus. In this group of plants, different variants were found in the isozymes of diaphorases and glutamate‐oxalacetate‐transammases. These variants might indicate that M.×giganteus includes more than one genotype. Some of the plants which were assumed to belong to M.×giganteus could clearly be excluded from this variety. Although some of the varieties can only be unequivocally identified by their flowers, it is possible to analyse the isozymes even in the dormant state of the rhizomes, thus, this method offers a simple way of identifymg Miscanthus varieties by the principle of exclusion, independent of the physiological state of the plant.

SSR-based genetic maps of Miscanthus sinensis and M. sacchariflorus, and their comparison to sorghum

“…Since the first molecular study in Miscanthus, using 13 isozymes for the estimation of genetic variation (Von Wuhlisch et al 1994), AFLP (Greef et al 1997;Hodkinson et al 2002b) and inter-simple sequence repeat (ISSR) (Hodkinson et al 2002b) have been used to evaluate the genetic diversity of Miscanthus species. The intergenic spacer (IGS) of the nuclear genes for ribosomal RNA (rDNA) (Chou et al 1999), RAPD (Chou et al 2000), the IGS between trnL and trnF genes of chloroplastic DNA (cpDNA) (Chiang et al 2003), RFLP (Iwata et al 2005), and microsatellite markers (Hung et al 2009) have also been implemented to study structures of natural populations of Miscanthus.…”

Miscanthus: a case study for the utilization of natural genetic variation

“…Several multi-locus marker systems have been applied to Miscanthus such as restriction fragment length polymorphism (RFLP; Hernández et al , 2001), randomly amplified polymorphic DNA (RAPD; Chiang et al , 2003), ISSR polymerase chain reaction (ISSR-PCR; Hodkinson et al, 2002c; Zhang et al, 2013a, b) and AFLP (Greef et al , 1997; Hodkinson et al , 2002c). Single-locus co-dominant markers have also been applied including isozymes (Chou et al , 1987; Chou and Chang, 1988; Chou and Ueng, 1992; Von Wühlisch et al , 1994). Many simple-sequence repeat (SSR) markers have been developed for the nuclear genome (Hernández et al , 2001; Hung et al , 2009; Ho et al , 2011; Zhou et al , 2011; Hu et al , 2012; Kim et al ., 2012; Yu et al, 2013), but fewer have been developed for the plastid/chloroplast genome (De Cesare et al , 2010; Jiang et al , 2012).…”

“…Single-locus co-dominant markers have also been applied including isozymes (Chou et al, 1987;Chou and Chang, 1988;Chou and Ueng, 1992;Von Wühlisch et al, 1994). Many simple-sequence repeat (SSR) markers have been developed for the nuclear genome (Hernández et al, 2001;Hung et al, 2009;Ho et al, 2011;Zhou et al, 2011;Hu et al, 2012;Kim et al, 2012;Yu et al, 2013), but fewer have been developed for the plastid/chloroplast genome (De Cesare et al, 2010;Jiang et al, 2012).…”