Identify miRNA-mRNA regulation pairs to explore potential pathogenesis of lung adenocarcinoma

Fan, Xingchen; Zou, Xuan; Liu, Cheng; Peng, Shuang; Zhang, Shiyu; Zhou, Xin; Zhu, Jun; Zhu, Wei

doi:10.18632/aging.204341

Cited by 5 publications

(5 citation statements)

References 37 publications

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“…We veri ed this nding in samples from 20 pairs of patients and a new miRNA sequence, miR-1246b has caught our attention. The AUC for the diagnosis of benign and malignant pulmonary nodules was only 0.741, which may be related to our small sample size; Furthermore, previous studies have also found that the diagnostic performance of multiple miRNAs is higher than that of a single miRNA in tumors [38,39]. Therefore, a follow-up study should be carried out to expand the sample, simultaneously, a combination with other miRNAs may be considered.…”

Section: Discussionmentioning

confidence: 82%

MiR-1246b, a novel miRNA molecule of extracellular vesicles in bronchoalveolar lavage fluid promotes the nodules growth through FGF14 in patients with lung cancers

Huang

Ding

Yuan

et al. 2023

Preprint

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With the widespread development of chest computed tomography (CT), the detection rate of pulmonary nodules has been increasing, therefore, the judgment of benign and malignant nodules has become a common problem in clinic. MicroRNA, a potential tool, is expected to become a good choice for diagnosing and studying the occurrence and development of diseases through the vector of bronchoalveolar lavage fluid extracellular vesicles (BALF-EVs). In this study, radial endobronchial ultrasound (R-EBUS) was used to locate pulmonary nodules in patients, BALF was obtained and EVs were isolated, and small RNA sequencing was performed to screen differential miRNAs between benign and malignant pulmonary nodules groups. The binding targets and underlying mechanisms of the differential miRNAs were verified by in vitro and in vivo experiments. EVs were successfully isolated and identified after obtaining BALF by R-EBUS localization. Differential miRNAs in BALF-EVs of patients with benign or malignant pulmonary nodules were screened by high-throughput small RNA sequencing. A new sequence, miR-1246b, was noticed. We found that FGF14 was the binding target of miR-1246b by luciferase Assay. Subsequent mechanistic studies showed that miR-1246b inhibited the expression of FGF14 in lung cancer cells, further led to ERK phosphorylation and epithelial-to-mesenchymal transition (EMT), which ultimately contributed to lung cancer cell proliferation, migration and invasion. In summary, our study demonstrates that the detection of miRNAs in BALF-EVs, a means of liquid biopsy, could assist to distinguish malignant nodules from benign nodules. miR-1246b, extracted from BALF-EVs, targets FGF14 to promote lung cancer cell proliferation, migration and invasion.

show abstract

Section: Discussionmentioning

confidence: 82%

MiR-1246b, a novel miRNA molecule of extracellular vesicles in bronchoalveolar lavage fluid promotes the nodules growth through FGF14 in patients with lung cancers

Huang

Ding

Yuan

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…The AUC for miR-1246b in the diagnosis of benign and malignant pulmonary nodules was only 0.743, which may be related to our small sample size. Furthermore, previous studies have found that the diagnostic performance of multiple miRNAs is higher than that of a single miRNA in tumors [ 38 , 39 ]. Therefore, a follow-up study should be carried out to expand the sample size; a combination with other miRNAs may also be considered.…”

Section: Discussionmentioning

confidence: 99%

MiR-1246b, a novel miRNA molecule of extracellular vesicles in bronchoalveolar lavage fluid, promotes nodule growth through FGF14 in patients with lung cancer

Huang,

Ding,

et al. 2023

Cell Death Dis

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With the widespread development of chest computed tomography (CT), the detection rate of pulmonary nodules has increased; therefore, the classification of benign vs. malignant nodules has become a common problem in the clinic. MicroRNA, a potential tool, is expected to become a good choice for diagnosing and studying the occurrence and development of diseases through the vector of bronchoalveolar lavage fluid extracellular vesicles (BALF-EVs). In this study, radial endobronchial ultrasound (R-EBUS) was used to locate pulmonary nodules in patients. BALF was obtained, EVs were isolated, and small RNA sequencing was performed to screen differentially expressed miRNAs between benign and malignant pulmonary nodules. The binding targets and underlying mechanisms of the differentially expressed miRNAs were verified by in vitro and in vivo experiments. R-EBUS localization and sampling was used to obtain BALF, and EVs were successfully isolated and characterized. Differentially expressed miRNAs in BALF-EVs of patients with benign vs. malignant pulmonary nodules were screened by high-throughput small RNA sequencing. A new miRNA, miR-1246b, was identified. We found that FGF14 was the binding target of miR-1246b by luciferase assay. Subsequent mechanistic studies showed that miR-1246b inhibited the expression of FGF14 in lung cancer cells, further leading to ERK phosphorylation and epithelial-to-mesenchymal transition (EMT), which ultimately contributed to lung cancer cell proliferation, migration and invasion. In summary, our study demonstrates that the detection of miRNAs in BALF-EVs, a means of liquid biopsy, could assist in distinguishing malignant nodules from benign nodules. miR-1246b, which was extracted from BALF-EVs, targets FGF14 to promote lung cancer cell proliferation, migration and invasion.

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“… 14 miRNA takes part in many biological processes of metabolism, cell differentiation, apoptosis, and disease control, involving multiple systems, including respiratory system, cardiovascular system, nervous system, and hematopoietic system. 15 Excessive studies have confirmed the correlation of miRNA with the growth and development of lung, as well as onset, development, and outcome of pulmonary fibrosis, pneumonia, lung cancer, chronic obstructive pulmonary disease, and so on. 16 , 17 Lin et al 8 demonstrated that miR-24 inhibited inflammatory response in neonatal rats with lipopolysaccharide-induced acute lung injury by targeting NLRP3.…”

Section: Discussionmentioning

confidence: 99%

Changes and Clinical Value of Serum miR-24 and miR-223 Levels in Patients with Severe Pneumonia

Gao,

Liu,

Zhang

et al. 2023

IJGM

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Introduction Severe pneumonia progresses rapidly, so early assessment of the severity and prognosis is crucial for reducing mortality rates. Objective We explore the role of serum microRNA-24 (miR-24) and microRNA-223 (miR-223) in the prognosis of severe pneumonia. Methods There were a total of 96 patients with general pneumonia, 94 patients with severe pneumonia, and 93 healthy people, who were enrolled in this study. The levels of serum miR-24 and miR-223 were detected by real-time fluorescent quantitative PCR in all groups. Results The serum miR-223 level in the severe group was higher than that in the common group and the control group, and the miR-24 level was lower than that in the common group and the control group (P<0.05). The serum miR-223 levels and APACHEII scores in the death group were higher than those in the survival group on the first, third, and seventh day after admission, while the miR-24 levels were lower than those in the survival group (P<0.05). The proportion of patients with mechanical ventilation in the death group was higher than that in the survival group (P<0.05). The level of serum miR-24 was negatively correlated with APACHEII score and mechanical ventilation in patients who died of severe pneumonia (P<0.05), and miR-223 was positively correlated with APACHEII score and mechanical ventilation (P<0.05). The AUC predicted by serum miR-24, miR-223, and APACHEII scores alone and jointly were 0.867, 0.839, 0.791, and 0.952, respectively. MiR-24 and miR-223 are protective and independent risk factors for mortality in severe pneumonia patients, respectively (P<0.05). MiR-24 was a protective factor affecting the death of patients with severe pneumonia, and miR-223 was an independent risk factor affecting the death of patients with severe pneumonia (P<0.05). Conclusion The combination of serum miR-24 and miR-223 levels on the first day after admission and APACHEII score can effectively predict prognosis.

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Identify miRNA-mRNA regulation pairs to explore potential pathogenesis of lung adenocarcinoma

Cited by 5 publications

References 37 publications

MiR-1246b, a novel miRNA molecule of extracellular vesicles in bronchoalveolar lavage fluid promotes the nodules growth through FGF14 in patients with lung cancers

MiR-1246b, a novel miRNA molecule of extracellular vesicles in bronchoalveolar lavage fluid promotes the nodules growth through FGF14 in patients with lung cancers

MiR-1246b, a novel miRNA molecule of extracellular vesicles in bronchoalveolar lavage fluid, promotes nodule growth through FGF14 in patients with lung cancer

Changes and Clinical Value of Serum miR-24 and miR-223 Levels in Patients with Severe Pneumonia

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