2006
DOI: 10.1099/vir.0.81603-0
|View full text |Cite
|
Sign up to set email alerts
|

Identifying cellular genes crucial for the reactivation of Kaposi's sarcoma-associated herpesvirus latency

Abstract: Kaposi's sarcoma-associated herpesvirus (KSHV) is the latest addition to the long list of human herpesviruses. Reactivation of latent herpesvirus infections is still a mystery. It was demonstrated recently that the phorbol ester TPA was efficient in inducing a reactivation of KSHV infection in the S phase of the cell cycle. In the present study, flow cytometry-sorted, TPA-induced, KSHV-infected haematopoietic cells (BCBL-1) were used to analyse the expression profiles of cancer-related cellular genes in the S … Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

2
32
1

Year Published

2006
2006
2017
2017

Publication Types

Select...
6
2
1

Relationship

0
9

Authors

Journals

citations
Cited by 22 publications
(35 citation statements)
references
References 50 publications
2
32
1
Order By: Relevance
“…Five genes were chosen for further investigation by RT-PCR 72 h after exposure of S11 or ANA-1/MHV-68 cells to NP. Ribonucleoside-diphosphate reductase large subunit (Rrm1) is described as being upregulated in the context of reactivation of the human gammaherpesviruses EBV and Kaposi’s sarcoma-associated herpesvirus (KSHV) [34] while Fructosamine-3-kinase (Fn3k), Tousled-like Kinase 1 (Tlk1), TGFbeta-activated kinase 1/MAP3K7-binding protein 1 (Tab1), and Sirtuin 1 (Sirt1) have been found to be downregulated [35, 36]. Consistent with reactivation of latent virus, Rrm1, Fn3k and Sirt1 were up- or downregulated as described for EBV and KSHV, albeit to a variable degree depending on the cell line and the type of NP (Fig.…”
Section: Resultsmentioning
confidence: 99%
“…Five genes were chosen for further investigation by RT-PCR 72 h after exposure of S11 or ANA-1/MHV-68 cells to NP. Ribonucleoside-diphosphate reductase large subunit (Rrm1) is described as being upregulated in the context of reactivation of the human gammaherpesviruses EBV and Kaposi’s sarcoma-associated herpesvirus (KSHV) [34] while Fructosamine-3-kinase (Fn3k), Tousled-like Kinase 1 (Tlk1), TGFbeta-activated kinase 1/MAP3K7-binding protein 1 (Tab1), and Sirtuin 1 (Sirt1) have been found to be downregulated [35, 36]. Consistent with reactivation of latent virus, Rrm1, Fn3k and Sirt1 were up- or downregulated as described for EBV and KSHV, albeit to a variable degree depending on the cell line and the type of NP (Fig.…”
Section: Resultsmentioning
confidence: 99%
“…293T cells were transfected with MirusTransIT reagent according to the manufacturer's protocol. For some oligonucleotide knockdown experiments in BCBL1 TReX-RTA cells, cultures were first synchronized into S phase by serum starvation for 24 hours, followed by the re-addition of serum and incubation for 16 hours [25], [26]. Cells were then electroporated with RNase H-targeting modified oligonucleotides and allowed to recuperate overnight in RPMI supplemented with 20% tetracycline-compatible FBS (Clonetech), followed by induction the next day with 0.2 ”g/mL doxycycline.…”
Section: Methodsmentioning
confidence: 99%
“…Microarrays were performed in two biological replicates for each group. Briefly, as described elsewhere [35], [36], [37], [38], [39], using the TrueLabeling-AMP 2.0 kit, 1 ”g of purified total RNA was reversely transcribed to obtain cDNA and converted into biotin-labeled cRNA using biotin-16-UTP (Roche) by in vitro transcription. The biotin-labeled cRNA was purified using the cRNA clean-up kit.…”
Section: Methodsmentioning
confidence: 99%