Identifying Differences Between Normal and Invasive Ductal Carcinoma grades for Breast tissues By FT-IR Spectroscopy

Abstract

Breast cancer is one of the most important malignant forms of cancers and it represents a great threat to life for women. FT-IR spectra ware taken for 98 samples of breast tissues that were previously histopathologically identified by pathologist experienced as: 63 normal (N) samples and 35 invasive ductal carcinoma (IDC) samples that were classified in different grades 19 G1 samples,7 G2samples and 9 G3 samples in Mid-IR frequency range between 400 cm -1 and 4000 cm -1 . Many spectral differences were observed in the frequency regions N-H stretching, Amide bands, C-H vibrations, and 950-1400 cm -1 . The aim of this study is analyze different grades of IDC spectroscopically to evaluate the efficiency of FT-IR spectroscopy to differentiate between these grades. The results show considerable decrease in the lipid and carbohydrate content with the carcinoma grades (from G1 to G3), while, protein, collagen and nucleic acid (DNA) reveal slight increase with the carcinoma grade, that will be useful in classifying three different nuclear grades. This study demonstrates that FT-IR spectroscopy is a promising tool for accurate, rapid diagnosis of breast canser.