Identifying genes and gene networks involved in chromium metabolism and detoxification in Crambe abyssinica

Zulfiqar, Asma; Paulose, Bibin; Chhikara, Sudesh; Dhankher, Om Parkash

doi:10.1016/j.envpol.2011.06.027

Cited by 20 publications

(9 citation statements)

References 29 publications

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“…ex Fries) in response to metalloid arsenate exposure and demonstrated that transcripts related to sulfated metabolism (SiR, ATPS) and glutathione synthase (GS) were induced under this abiotic stress. Similarly, Zulfiqar et al noted that genes regulating the sulfur assimilation and glutathione biosynthesis were upregulated in C. abyssinica upon exposure to chromium.…”

Section: Resultsmentioning

confidence: 90%

Physiological and Molecular Response ofArabidopsis thaliana(L.) to Nanoparticle Cerium and Indium Oxide Exposure

Chhikara

Xing

et al. 2013

ACS Sustainable Chem. Eng.

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The effects of cerium oxide (CeO 2 ) and indium oxide (In 2 O 3 ) nanoparticles (NPs) exposure on Arabidopsis thaliana (L.) Heynh. were investigated. After inoculation in half strength MS medium amended with 0−2000 ppm CeO 2 and In 2 O 3 NPs for 25 days, both physiological and molecular responses were evaluated. Exposure at 250 ppm CeO 2 NPs significantly increased plant biomass, but at 500−2000 ppm, plant growth was decreased by up to 85% in a dose-dependent fashion. At 1000 and 2000 ppm CeO 2 NPs, chlorophyll production was reduced by nearly 60% and 85%, respectively, and anthocyanin production was increased 3−5-fold. Malondialdehyde (MDA) production, a measure of lipid peroxidation, was unaffected by exposure to 250−500 ppm CeO 2 NPs, but at 1000 ppm, MDA formation was increased by 2.5-fold. Exposure to 25−2000 ppm In 2 O 3 NPs had no effect on A. thaliana biomass and only minor effects (15%) on root elongation. Total chlorophyll and MDA production were unaffected by In 2 O 3 NPs exposure. Molecular response to NP exposure as measured by qPCR showed that both types of elements altered the expression of genes central to the stress response such as the sulfur assimilation and glutathione (GSH) biosynthesis pathway, a series of genes known to be significant in the detoxification of metal toxicity in plants. Interestingly, In 2 O 3 NPs exposure resulted in a 3.8−4.6-fold increase in glutathione synthase (GS) transcript production, whereas CeO 2 NPs yielded only a 2-fold increase. It seems likely that the significantly greater gene regulation response upon In 2 O 3 NPs exposure was directly related to the decreased phytotoxicity relative to CeO 2 treatment. The use of NP rare earth oxide elements has increased dramatically, yet knowledge on fate and toxicity has lagged behind. To our knowledge, this is the first report evaluating both physiological and molecular plant response from exposure to these important nanoparticles. KEYWORDS: Arabidopsis thaliana, CeO 2 and In 2 O 3 NPs, Stress response, Gene expression, Anthocyanin, Lipid peroxidation, Sulfur assimilation pathway coli, Bacillus subtilis, and Pseudomonas f luorescens), 5,6 plants (Cucurbita pepo L., Solanum lycopersicum L., and Zea mays L.) 7−9 and animals (Zebrafish: Danio rerio). 10,11 Moreover, the potential hazards of nanoparticles to human health have been discussed in assays using human cells. 4,12 Given these findings, it is clear that a full and mechanistic understanding of the risks Special Issue: Sustainable Nanotechnology

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Section: Resultsmentioning

confidence: 90%

Physiological and Molecular Response ofArabidopsis thaliana(L.) to Nanoparticle Cerium and Indium Oxide Exposure

Chhikara

Xing

et al. 2013

ACS Sustainable Chem. Eng.

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228

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“…In soybean at 400 and 500 ppm Cr concentration there was about 83% and 85% reduction in length of seedling respectively [48]. With Crambe the fresh weight of plants decreased moderately at 100 μM K 2 CrO 4 , whereas at 150 μM K 2 CrO 4 there was a significant reduction in biomass with no symptoms of severe cellular damage, but at higher concentration (200 and 250 μM), plant showed chlorosis and visible necrosis on leaves [49]. Also in Crambe, root length was decreased by 41.33%, shoot length by 15.66% and fresh seedling weight by 27.67% at 0.05 mM Cr [26].…”

Section: The Influence Of Crmentioning

confidence: 99%

Influence of Heavy Metals on Seed Germination and Early Seedling Growth in <i>Eruca sativa</i> Mill.

Zhi¹,

Deng²,

Luo³

et al. 2015

AJPS

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Heavy metals present in soil and water naturally or as contaminants from human activities can cause bioaccumulation affecting the entire ecosystem and pose harmful health consequences in all life forms. Some famous non-food hyperaccumulators such as Thlaspi caerulescens, Sedum alfredii, Pteris vittata, Arabidopsis halleri and Athyrium yokoscense are of very little economic value, making it difficult for them to be used for phytoremediation. In this paper, the influence of heavy metals Cu, Ni, Zn, Hg, Cr, Pb and Cd on seed germination and early seedling growth in oil crop Eruca sativa was evaluated under laboratory conditions. Our results indicated that among the 7 heavy metals tested, only Ni at higher concentrations (1 mM and above) significantly decreased the Eruca seed germination in a dose-dependent manner. All heavy metals except Zn and Ni decreased the root length first, then the shoot length or the fresh seedling weight, and seed germination was always the last to be influenced. With Ni, the root length, shoot length and fresh seedling weight were stimulated when Ni concentrations were under 1 mM; with Zn, the root length, shoot length and fresh seedling weight were increased by all concentrations tested (0.20 -5.0 mM). Our results indicated that Eruca is tolerant or moderately tolerant to Cu, Hg, Cr, and Cd and highly tolerant to Pb, Ni and Zn, and can be developed as an industrial oil crop for phytoremediation of soils contaminated by heavy metals.

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“…In our experiment Crambe seed germination was not significantly decreased by Cr concentrations tested (0.05 -0.80 mM or 2.6 -41.6 mg•L −1 ), suggesting that Crambe seed germination is tolerant to Cr (Figure 1). Zulfiqar et al [38] reported that Crambe fresh weight of plants was decreased moderately at 100 μM K 2 CrO 4 , whereas at 150 μM K 2 CrO 4 there was a significant reduction in biomass with no symptoms of severe cellular damage, but at higher concentration (200 and 250 μM), plant showed chlorosis and visible necrosis on leaves. In our experiment Crambe root length was decreased by 41.33% (Figure 2), shoot length by 15.66% (Figure 3) and fresh seedling weight by 27.67% (Figure 4) at 0.05 mM Cr, suggesting that Crambe is only moderately tolerant to Cr regarding early seedling growth.…”

Section: The Influence Of Crmentioning

confidence: 99%

Influence of Heavy Metals on Seed Germination and Early Seedling Growth in Crambe abyssinica, a Potential Industrial Oil Crop for Phytoremediation

Hu¹,

Deng²,

Wang³

et al. 2015

AJPS

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The influence of essential (Cu, Ni and Zn) and non-essential heavy metals (Hg, Cr, Pb and Cd) on seed germination and early seedling growth in industrial oil crop Crambe abyssinica was evaluated under laboratory conditions. Our results indicated that among the 7 heavy metals tested only Cu and Hg significantly (P < 0.01) decreased Crambe seed germination in a dose-dependent manner at higher concentrations while certain Cr concentrations significantly increased the seed germination (P < 0.05). All the 7 heavy metals decreased significantly relative root length, shoot length and fresh seedling weight in a dose-dependent manner (P < 0.01). The heavy metals except Ni decreased relative root length first, then shoot length or fresh seedling weight, and finally seed germination. Ni seemed to influence the relative fresh seedling weight first, then shoot length, root length and finally seed germination at lower concentrations, but the decrease in relative root length became faster when the Ni concentrations were increased. Our results indicated that Crambe is tolerant or moderately tolerant to the heavy metals tested except Ni and can be improved for phytoremediation of soils contaminated by heavy metals.

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Identifying genes and gene networks involved in chromium metabolism and detoxification in Crambe abyssinica

Cited by 20 publications

References 29 publications

Physiological and Molecular Response ofArabidopsis thaliana(L.) to Nanoparticle Cerium and Indium Oxide Exposure

Physiological and Molecular Response ofArabidopsis thaliana(L.) to Nanoparticle Cerium and Indium Oxide Exposure

Influence of Heavy Metals on Seed Germination and Early Seedling Growth in <i>Eruca sativa</i> Mill.

Influence of Heavy Metals on Seed Germination and Early Seedling Growth in Crambe abyssinica, a Potential Industrial Oil Crop for Phytoremediation

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Identifying genes and gene networks involved in chromium metabolism and detoxification in Crambe abyssinica

Cited by 20 publications

References 29 publications

Physiological and Molecular Response ofArabidopsis thaliana(L.) to Nanoparticle Cerium and Indium Oxide Exposure

Physiological and Molecular Response ofArabidopsis thaliana(L.) to Nanoparticle Cerium and Indium Oxide Exposure

Influence of Heavy Metals on Seed Germination and Early Seedling Growth in &lt;i&gt;Eruca sativa&lt;/i&gt; Mill.

Influence of Heavy Metals on Seed Germination and Early Seedling Growth in Crambe abyssinica, a Potential Industrial Oil Crop for Phytoremediation

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Influence of Heavy Metals on Seed Germination and Early Seedling Growth in <i>Eruca sativa</i> Mill.