Identifying Human Herpesvirus 8 Infection: Performance Characteristics of Serologic Assays

Engels, Eric A.; Whitby, Denise; Goebel, P. Bradley; Stossel, Andrea; Waters, David J.; Pintus, A.; Contu, L.; Biggar, Robert J.; Goedert, James J.

doi:10.1097/00042560-200004010-00011

Cited by 61 publications

(63 citation statements)

References 23 publications

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“…This is more likely given the limited sensitivity of KSHV serologic assays. 28 A third explanation is that these women were acutely infected with KSHV and had not yet seroconverted. Since follow-up samples are not available, it is not possible to test this possibility.…”

Section: Discussionmentioning

confidence: 99%

“…A latent IFA measured antibodies to the latent nuclear antigen (LANA or LNA-1) encoded by orf73, while a recombinant protein ELISA measured antibodies in the lyticphase glycoprotein K8.1 as previously described, 28 using a cutoff of OD 1.0. For the multivariate statistical analysis, women with a positive result to either of the studied antigens were considered seropositive.…”

Section: Serology: Kshv and Hivmentioning

confidence: 99%

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Prevalence of Kaposi's sarcoma‐associated herpesvirus infection in sex workers and women from the general population in Spain

Sanjosé

Marshall

Solá

et al. 2001

Intl Journal of Cancer

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Transmission routes of Kaposi's sarcoma-associated herpesvirus (KSHV) in the general population are poorly understood. Whereas sexual transmission appears to be common in homosexual men, the evidence for heterosexual transmission is less convincing. In our study, prevalence of KSHV infection was examined among women in the Spanish general population and among sex workers. Subjects consisted of 100 prostitutes and 100 women randomly sampled from the general population and age-matched to the prostitutes. Women had a personal interview and gynecologic examinations in which a blood sample, cervical cells and oral cells were obtained. Peripheral blood mononuclear cells (PBMC), oral and cervical samples were tested for KSHV DNA by quantitative real-time PCR. Sera were tested for antibodies against human immunodeficiency virus (HIV) by ELISA and against KSHV by latent IFA and K8.1 ELISA. Women who were positive in either serologic assay or PCR were considered infected by KSHV. Human papillomavirus (HPV) DNA in cervical scrapes were evaluated using the Hybrid Capture System™. The study population had an average age of 30 years and were HIV-negative. Women from the general population were largely of Spanish nationality, and 61% reported lifetime monogamy. The majority of the prostitutes (76%) were immigrants, primarily from South America. Sex workers were twice as likely to be infected with KSHV than women in the general population (16% vs. 8%, prevalence odds ratio [OR] ‫؍‬ 2.2). KSHV was more prevalent among HPV DNA-positive women (OR ‫؍‬ 2.5) and among women with an early age at first sexual intercourse (OR ‫؍‬ 2.7, p < 0.05). KSHV DNA was detected by PCR in 3% of the oral cavity samples, in 2% of the cervical samples of the prostitutes and in 1% of the cervical samples of women in the general population. All PBMC samples were negative. These results suggest that in low-risk countries for KSHV, oral shedding and heterosexual contacts are potential pathways for KSHV transmission. © 2002 Wiley-Liss, Inc. Key words: KSHV; HHV-8; HPV DNA; epidemiology; prostitutionKaposi's sarcoma-associated herpesvirus (KSHV), also known as human herpesvirus 8 (HHV-8), has been shown to be causally associated with Kaposi's sarcoma and other diseases. 1-3 The natural history of KSHV and its routes of transmission are not well understood. Epidemiologic studies have shown that seroprevalence of KSHV antibodies is more than 25% in African countries, whereas in the United States and Europe is lower than 10%. 4 Data from Latin America are limited but suggest a wide variability within the region. 5,6 Routes of transmission may vary in different geographic areas either reflecting or possibly leading to variations in background prevalence. Nonsexual transmission of KSHV has been reported to be the major route of transmission in countries with high KSHV prevalence rates where infection in children is commonly reported. 6 -9 In countries with lower background prevalence, sexual transmission, at least among homosexual men, may be predominant. Among...

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Section: Discussionmentioning

confidence: 99%

Section: Serology: Kshv and Hivmentioning

confidence: 99%

Prevalence of Kaposi's sarcoma‐associated herpesvirus infection in sex workers and women from the general population in Spain

Sanjosé

Marshall

Solá

et al. 2001

Intl Journal of Cancer

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“…However, the included children were otherwise representative of the original survey population. Anti-HHV-8 antibodies were measured by using an enzyme immunoassay to K8.1 glycoprotein (a lytic-phase antigen) as previously reported (22,23). Antischistosomal antibodies were measured by using enzyme-linked immunoelectrotransfer blots (EITB) to detect species-specifi c antibodies against microsomal glycoprotein antigens from Schistosoma hematobium and S. mansoni (98% sensitivity and 99% specifi city) (24).…”

Section: Patient Selection and Serologic Testing For Hhv-8 And Schistmentioning

confidence: 99%

Seroprevalence and Risk Factors for Human Herpesvirus 8 Infection, Rural Egypt1

Mbulaiteye

Pfeiffer

Dolan

et al. 2008

Emerg. Infect. Dis.

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To determine whether human herpesvirus 8 (HHV-8) is associated with schistosomal and hepatitis C virus infections in Egypt, we surveyed 965 rural household participants who had been tested for HHV-8 and schistosomal infection (seroprevalence 14.2% and 68.6%, respectively, among those <15 years of age, and 24.2% and 72.8%, respectively, among those ≥15 years of age. Among adults, HHV-8 seropositivity was associated with higher age, lower education, dental treatment, tattoos, >10 lifetime injections, and hepatitis C virus seropositivity. In adjusted analyses, HHV-8 seropositivity was associated with dental treatment among men (odds ratio [OR] 2.4, 95% confi dence interval [CI] 1.1-5.2) and hepatitis C virus seropositivity among women (OR 3.3, 95% CI 1.4-7.9). HHV-8 association with antischistosomal antibodies was not signifi cant for men (OR 2.1, 95% CI 0.3-16.4), but marginal for women (OR 1.5, 95% CI 1.0-2.5). Our fi ndings suggest salivary and possible nosocomial HHV-8 transmission in rural Egypt.

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“…Antibodies against gpK8.1A are important serological markers for KSHV disease. 59 Antibody profiling for the various KSHV diseases has demonstrated that serum antibodies against gpK8.1A are 5-fold higher in patients with MCD compared with KS and PEL.…”

Section: -50mentioning

confidence: 99%

Selective killing of Kaposi's sarcoma-associated herpesvirus lytically infected cells with a recombinant immunotoxin targeting the viral gpK8.1A envelope glycoprotein

Chatterjee

Chandran

Berger

2012

mAbs

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Identifying Human Herpesvirus 8 Infection: Performance Characteristics of Serologic Assays

Cited by 61 publications

References 23 publications

Prevalence of Kaposi's sarcoma‐associated herpesvirus infection in sex workers and women from the general population in Spain

Prevalence of Kaposi's sarcoma‐associated herpesvirus infection in sex workers and women from the general population in Spain

Seroprevalence and Risk Factors for Human Herpesvirus 8 Infection, Rural Egypt1

Selective killing of Kaposi's sarcoma-associated herpesvirus lytically infected cells with a recombinant immunotoxin targeting the viral gpK8.1A envelope glycoprotein

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