Identifying key genes involved in yellow leaf variation in ‘Menghai Huangye’ based on biochemical and transcriptomic analysis

Liu, Yufei; Pang, Dan; Jiang, Hong-Yan; Chen, Chunlin; Sun, Yanxia; Tian, Yaping; Xu, Yan; Song, Weixi; Chen, Linbo

doi:10.1007/s10142-022-00829-9

Cited by 9 publications

(9 citation statements)

References 35 publications

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“…While, for light-sensitive tea mutants, strong sunlight up-regulated the expression of genes involved in the carotenoid pathway, resulting in the accumulation of carotenoids, and strong sunlight induced hypoplasia of chloroplasts by suppressing the development of grana stacking and thylakoids, so the lack of chlorophylls and the accumulation of carotenoids were considered to cause the yellow color formation in etiolated shoots [ 10 ]. Similar research results have been reported in some etiolated mutants, for example, ‘Anji baicha’ [ 26 ], ‘Huabai 1’ [ 13 ], ‘Xiaoxueya’ [ 27 ], ’Yujinxiang’ [ 28 ], ‘Huangjinya’ [ 20 ], and ‘Menghaihuangya’ [ 29 ]. The current study results showed that the total chlorophyll content of normal green leaves of ‘Yinghong 9’ was significantly higher than that of yellow leaves of ‘Huangyu’ ( Figure 1 B).…”

Section: Discussionsupporting

confidence: 81%

Metabolic and Transcriptomic Profiling Reveals Etiolated Mechanism in Huangyu Tea (Camellia sinensis) Leaves

Mei

Zhang

Yang

et al. 2022

IJMS

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Leaf color is one of the key factors involved in determining the processing suitability of tea. It relates to differential accumulation of flavor compounds due to the different metabolic mechanisms. In recent years, photosensitive etiolation or albefaction is an interesting direction in tea research field. However, the molecular mechanism of color formation remains unclear since albino or etiolated mutants have different genetic backgrounds. In this study, wide-target metabolomic and transcriptomic analyses were used to reveal the biological mechanism of leaf etiolation for ‘Huangyu’, a bud mutant of ‘Yinghong 9’. The results indicated that the reduction in the content of chlorophyll and the ratio of chlorophyll to carotenoids might be the biochemical reasons for the etiolation of ‘Huangyu’ tea leaves, while the content of zeaxanthin was significantly higher. The differentially expressed genes (DEGs) involved in chlorophyll and chloroplast biogenesis were the biomolecular reasons for the formation of green or yellow color in tea leaves. In addition, our results also revealed that the changes of DEGs involved in light-induced proteins and circadian rhythm promoted the adaptation of etiolated tea leaves to light stress. Variant colors of tea leaves indicated different directions in metabolic flux and accumulation of flavor compounds.

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Section: Discussionsupporting

confidence: 81%

Metabolic and Transcriptomic Profiling Reveals Etiolated Mechanism in Huangyu Tea (Camellia sinensis) Leaves

Mei

Zhang

Yang

et al. 2022

IJMS

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“…That is because the teas produced from the white or white tender shoots contains much higher theanine and lower polyphenols, and therefore have an improved ‘umami’ taste and weak bitterness and stringency[ 17 ]. It was proposed that mutations in genes encoding proteins in photosynthetic pigment biosynthesis and degradation,the chloroplastic DOXP/MEP pathway, and chloroplast-nucleus signaling, are responsible for the albino phenotype of tea plants[ 18 – 20 ]. However, why theanine highly accumulates in albino andetiolated tea plants is still largely unknown.…”

Section: Introductionmentioning

confidence: 99%

Haem Oxygenase 1 is a potential target for creating etiolated/albino tea plants (Camellia sinensis) with high theanine accumulation

Chen

Lin

Chen

et al. 2022

Horticulture Research

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Theanine content is highly correlated with sensory quality and health benefits of tea infusion. The tender shoots of etiolated and albino tea plants contain higher theanine than the normal green tea plants and are valuable materials for high quality green tea processing. However, why these etiolated or albino tea plants can highly accumulate theanine is largely unknown. In this study, we observed an Arabidopsis etiolated mutant hy1-100 (mutation in Haem Oxygenase 1, HO1) accumulated higher levels of glutamine (an analog of theanine). We therefore identified CsHO1 in tea plants, and found CsHO1 is conserved in amino acid sequences and subcellular localization with its homologs in other plants. Importantly, CsHO1 expression in the new shoots was much lower in an etiolated tea plants “Huangkui” and an albino tea plant “Huangshan Baicha” than that in normal green tea plants. The expression levels of CsHO1 were negatively correlated with theanine contents in these green, etiolated and albino shoots. Moreover, CsHO1 expression levels in various organs and different time points were also negatively correlated with theanine accumulation. The hy1-100 was hypersensitive to high level of theanine and accumulated more theanine under theanine feeding, and these phenotypes were rescued by the expression of CsHO1 in this mutant. Transient knock-down CsHO1 expression in the new shoots of tea plant using antisense oligonucleotides (asODN) increased theanine accumulation. Collectively, these results demonstrated CsHO1 negatively regulates theanine accumulation in tea plants, and that low expression CsHO1 likely contributes to the theanine accumulation in etiolated/albino tea plants.

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“…Researchers have found that albino tea plants could be classified as light-sensitive, temperature-sensitive, and ecologically insensitive ( Zhang et al., 2020c ). Their chloroplast development ( Gao et al., 2021 ), leaf structure ( Du et al., 2008 ), metabolic profiles ( Li et al., 2015a ), and gene expression profiles ( Liu et al., 2022 ) were also discussed in various publications. Purple leaf variations, such as ‘Mooma1’, are highly accumulated with anthocyanins, resulting in unique appearances and flavor.…”

Section: Resultsmentioning

confidence: 99%

Topics and trends in fresh tea (Camellia sinensis) leaf research: A comprehensive bibliometric study

Chen

Shen

et al. 2023

Front. Plant Sci.

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Tea plant (Camellia sinensis) is a widely cultivated cash crop and tea is a favorite functional food in the world. Fresh tea leaves (FTLs) play a critical role in bridging the two fields closely related to tea cultivation and tea processing, those are, tea plant biology and tea biochemistry. To provide a comprehensive overview of the development stages, authorship collaboration, research topics, and hotspots and their temporal evolution trends in the field of FTLs research, we conducted a bibliometric analysis, based on 971 publications on FTLs-related research published during 2001-2021 from Web of Science Core Collection. CiteSpace, R package Bibliometrix, and VOSviewer were employed in this research. The results revealed that the development history can be roughly divided into three stages, namely initial stage, slow development stage and rapid development stage. Journal of Agricultural & Food Chemistry published most articles in this field, while Frontiers in Plant Science held the highest total citations and h-index. The most influential country, institution, and author in this field was identified as China, the Chinese Academy of Agricultural Sciences, and Xiaochun Wan, respectively. FTLs-related research can be categorized into three main topics: the regulation mechanism of key genes, the metabolism and features of essential compounds, and tea plants’ growth and stress responses. The most concerning hotspots are the application of advanced technologies, essential metabolites, leaf color variants, and effective cultivation treatments. There has been a shift from basic biochemical and enzymatic studies to studies of molecular mechanisms that depend on multi-omics technologies. We also discussed the future development in this field. This study provides a comprehensive summary of the research field, making it easier for researchers to be informed about its development history, status, and trends.

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Identifying key genes involved in yellow leaf variation in ‘Menghai Huangye’ based on biochemical and transcriptomic analysis

Cited by 9 publications

References 35 publications

Metabolic and Transcriptomic Profiling Reveals Etiolated Mechanism in Huangyu Tea (Camellia sinensis) Leaves

Metabolic and Transcriptomic Profiling Reveals Etiolated Mechanism in Huangyu Tea (Camellia sinensis) Leaves

Haem Oxygenase 1 is a potential target for creating etiolated/albino tea plants (Camellia sinensis) with high theanine accumulation

Topics and trends in fresh tea (Camellia sinensis) leaf research: A comprehensive bibliometric study

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