Parasitic infections are common in sheep farming worldwide. A cross-sectional study was carried out to determine the prevalence and associated risk factors among gastrointestinal parasites and sheep herds from the Brazilian Pampa biome, Rio Grande do Sul state, Brazil. Twenty-one farms were visited, where rectal faecal samples were individually collected from 309 animals. The sheep industry on the studied farms was characterised by small farms with a low level of technification, an extensive grazing system and meat production. Of all samples analysed, strongyle nematodes had the highest prevalence (77.02%), followed by
Eimeria
spp. (70.55%),
Moniezia expansa
(20.39%) and
Strongyloides papillosus
(17.48%). Mixed infection, between helminths and protozoans, was detected in 68.61% of samples. All herds were positive for strongyle and
Eimeria
spp. A predominance of
Haemonchus
spp. and
Trichostrongylus
spp. nematodes was observed in the herds. Younger animals were significantly more affected by
Eimeria
and
M. expansa
. In the semi-intensive and intensive systems, a higher frequency of
Eimeria
and strongyle infections was observed. Parasite infection was significantly reduced at low animal densities. The Brazilian Pampa region presents a high prevalence of gastrointestinal parasites among sheep; age, breeding system and stocking density were factors associated with parasite infection.