Identifying ligninolytic bacteria for lignin valorization to bioplastics

Xu, Tao; Zong, Qiu-Jin; He, Liu; Wang, Li; Liu, Zhihua; Li, Bingzhi; Yuan, Ying‐Jin

doi:10.1016/j.biortech.2022.127383

Cited by 25 publications

(10 citation statements)

References 36 publications

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“…The composition of corn stover and APL was analyzed according to the Laboratory Analytical Procedures of the National Renewable Energy Laboratory. , The concentrations of p -CA and FA in the fermentation broth were analyzed using high-pressure liquid chromatography (HPLC) (Shimadzu PDA, Japan) . For the analysis of fatty acids, glucose, acetic acid, and glycerol, an HPLC system equipped with a Bio-Rad HPX-87H column was employed.…”

Section: Methodsmentioning

confidence: 99%

Valorizing Lignin and Coprecursors into Homogeneous Polyhydroxyalkanoates by Engineered Pseudomonas putida

Wang,

Liu,

et al. 2024

ACS Sustainable Chem. Eng.

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Lignin, the largest renewable aromatic resource, holds great potential for valorization into valuable products. Biotransforming lignin into polyhydroxyalkanoate (PHA) can play a crucial role in addressing environmental concerns and fostering a sustainable bioeconomy. However, achieving homogeneity of PHA and high bioconversion efficiency of lignin are primary challenges. Engineering and precursor control strategies were thus designed to enhance the homogeneous PHA synthesis from lignin. The results showed that using precursors from lignin hydrolysate can boost PHA accumulation and regulate its monomer composition by Pseudomonas putida KT2440. Engineered P. putida KTYY06 was constructed by blocking PHA degradation, enhancing PHA synthesis and rewriting the fatty acid β-oxidation pathway. A remarkable homogeneous PHA containing 91.4% 3-hydroxyoctanoate monomer was achieved from co-feeding lignin-derived aromatics and precursors. Implementing a fed-batch fermentation strategy led to the highest PHA titer of 2.46 g/L, representing a 143.6% increase over the batch mode. P. putida KTYY06 also exhibited efficient PHA production, achieving an 85% 3-hydroxyoctanoate monomer content when using actual lignin streams. Overall, these multidimensional approaches of genetic modification, precursor regulation, and fermentation optimization show great promise for generating high-homogeneity PHA. The implications of this novel production strategy are significant, offering tailored PHAs with unique properties for advanced applications.

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Section: Methodsmentioning

confidence: 99%

Valorizing Lignin and Coprecursors into Homogeneous Polyhydroxyalkanoates by Engineered Pseudomonas putida

Wang,

Liu,

et al. 2024

ACS Sustainable Chem. Eng.

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“…Some characteristic peaks of lignin were found at 1218 cm −1 . β- O -4 and Cβ disappear [ 40 ] Pseudomonas putida A514 Alkali lignin 1%, – Extent of lignin degradation: 27% [ 41 ] Pseudomonas putida KT3-1 Alkali pretreated lignin (APL) 11.7 g/L, 72 h p -Hydroxybenzoic acid accumulated 20.90% PHA, β- O -4 linkage and small molecule degradation [ 42 ] Pseudomonas putida B6-2 Alkali pretreated lignin (APL) 11.7 g/L, 72 h Extent of vanillic acid degradation: 25.40% (72 h); extent of ferulic acid degradation: 100% (48 h); the molar conversion extent of ferulic acid into vanillic acid was 57.20% Acinetobacter Nocardia autotrophica Nitrated pine lignin –, 20 min Absorbance increases at 430 nm [ 16 ] Acinetobacter PC/4 Nitrated wheat lignin –, 20 min Absorbance increases at 430 nm Acinetobacter sp. ADP1 Alkali pretreatment of lignin 10.5 g/L, 168 h Extent of lignin degradation: 20–25%; absorbance increased at 280 nm [ 22 ] Acinetobacter johnsonii LN2 Alkaline lignin –, 72 h Extent of lignin degradation: 38.80% [ 17 ] Acinetobacter lwoffii LN4 Alkaline lignin –,72 h Extent of lignin degradation: 52% Cupriavidus Cupriavidus necator H16 Alka...…”

Section: Lignin-degrading Bacteriamentioning

confidence: 99%

“…280 nm absorbance increased; degradation extent of aromatic compounds: 19.40%, [ 38 ] Ochrobactrum pseudintermedium B-04 Sodium lignosulfonate 1 g/L, 168 h Extent of lignin degradation: 4.86% [ 13 ] Pandoraea Pandoraea norimbergensis LD001 Sulfate lignin 0.5 g/L, 72 h Lignin dye decolorization [ 39 ] Burkholderia Burkholderia sp . H801 Sulfate lignin 1 g/L, 144 h Extent of lignin degradation: 49.80% [ 23 ] Burkholderia cepacia B1-2 Alkaline Pretreatment Solution (APL) 11.7 g/L, 48 h Ferulic acid and vanillic acid are completely consumed [ 42 ] Burkholderia sp . H1 Wheat straw lignin 3.0%( w / v ), 168 h Extent of lignin degradation: 6.74% [ 44 ] Lanorella Raoultella ornithinolytica RS-1 Corn straw lignin –, 168 h Extent of lignin degradation: 19%; C=O tensile vibration of aromatic ring [ 45 ] Serratia Serratia sp .…”

Section: Lignin-degrading Bacteriamentioning

confidence: 99%

Bacterial transformation of lignin: key enzymes and high-value products

Gu,

Qiu,

et al. 2024

Biotechnol Biofuels

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Lignin, a natural organic polymer that is recyclable and inexpensive, serves as one of the most abundant green resources in nature. With the increasing consumption of fossil fuels and the deterioration of the environment, the development and utilization of renewable resources have attracted considerable attention. Therefore, the effective and comprehensive utilization of lignin has become an important global research topic, with the goal of environmental protection and economic development. This review focused on the bacteria and enzymes that can bio-transform lignin, focusing on the main ways that lignin can be utilized to produce high-value chemical products. Bacillus has demonstrated the most prominent effect on lignin degradation, with 89% lignin degradation by Bacillus cereus. Furthermore, several bacterial enzymes were discussed that can act on lignin, with the main enzymes consisting of dye-decolorizing peroxidases and laccase. Finally, low-molecular-weight lignin compounds were converted into value-added products through specific reaction pathways. These bacteria and enzymes may become potential candidates for efficient lignin degradation in the future, providing a method for lignin high-value conversion. In addition, the bacterial metabolic pathways convert lignin-derived aromatics into intermediates through the “biological funnel”, achieving the biosynthesis of value-added products. The utilization of this “biological funnel” of aromatic compounds may address the heterogeneous issue of the aromatic products obtained via lignin depolymerization. This may also simplify the separation of downstream target products and provide avenues for the commercial application of lignin conversion into high-value products.

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“…However, it remains difficult to draw such conclusions from data in the scientific literature, since variations in the source of lignin, organisms, and cultivation conditions dictate the composition of the resulting community and the extent of lignin depolymerization (Mendes et al, 2021;Azubuike et al, 2022). It has been demonstrated that certain microbes can degrade specific C-C and C-O bonds in lignin while also changing the content of H, G, and S lignin moieties (Bugg et al, 2020;Xu et al, 2022;Zhu et al, 2022). Similar in-depth structural characterizations of the effects of treatments with microbial consortia are currently lacking and future structural studies will be critical to progress in the field.…”

Section: Studies On Natural Microbial Consortiamentioning

confidence: 99%

Prospects for utilizing microbial consortia for lignin conversion

Rodríguez¹,

Hirakawa²,

Geiselman³

et al. 2023

Front. Chem. Eng.

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Naturally occurring microbial communities are able to decompose lignocellulosic biomass through the concerted production of a myriad of enzymes that degrade its polymeric components and assimilate the resulting breakdown compounds by members of the community. This process includes the conversion of lignin, the most recalcitrant component of lignocellulosic biomass and historically the most difficult to valorize in the context of a biorefinery. Although several fundamental questions on microbial conversion of lignin remain unanswered, it is known that some fungi and bacteria produce enzymes to break, internalize, and assimilate lignin-derived molecules. The interest in developing efficient biological lignin conversion approaches has led to a better understanding of the types of enzymes and organisms that can act on different types of lignin structures, the depolymerized compounds that can be released, and the products that can be generated through microbial biosynthetic pathways. It has become clear that the discovery and implementation of native or engineered microbial consortia could be a powerful tool to facilitate conversion and valorization of this underutilized polymer. Here we review recent approaches that employ isolated or synthetic microbial communities for lignin conversion to bioproducts, including the development of methods for tracking and predicting the behavior of these consortia, the most significant challenges that have been identified, and the possibilities that remain to be explored in this field.

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Identifying ligninolytic bacteria for lignin valorization to bioplastics

Cited by 25 publications

References 36 publications

Valorizing Lignin and Coprecursors into Homogeneous Polyhydroxyalkanoates by Engineered Pseudomonas putida

Valorizing Lignin and Coprecursors into Homogeneous Polyhydroxyalkanoates by Engineered Pseudomonas putida

Bacterial transformation of lignin: key enzymes and high-value products

Prospects for utilizing microbial consortia for lignin conversion

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