Identifying miRNA/mRNA negative regulation pairs in colorectal cancer

Abstract

Although considerable progress has been made in the molecular biology of Colorectal cancer (CRC), novel approaches are still required to uncover the detailed molecular mechanism of CRC. We aim to explore the potential negatively regulated miRNA-mRNA pairs and investigate their regulatory roles so as to elaborate the potential roles of the critical proteins in the signaling pathways enriched by the differential target genes of negatively regulated miRNA in CRC. Firstly, the differential miRNA-mRNA pairs were selected, followed by pairs of miRNA and their target genes. The obtained relationships were subjected to do functional enrichment analysis and those enriched in CRC pathways were chose to further construct a protein interaction network. Finally, we analyzed the regulatory roles of these relationships and constructed a regulatory network of negatively regulated miRNA and mRNA relationships. A total of 372 pairs of miRNA-mRNA were found and 108 target genes of miRNA were obtained. Three miRNAs including hsa-mir-23b, hsa-mir-365-1 and hsa-mir-365-2 showed significant influence on prognosis of CRC patients. To conclude, the miRNA/mRNA deregulations pairs identified in this study have high potentials to be further applied in diagnosis and treatment of CRC.

An miRNA Expression Signature for the Human Colonic Stem Cell Niche Distinguishes Malignant from Normal Epithelia

“…Among them, we found miR-23b expression was increased in primary colorectal cancers and in ALDH þ SC population from colorectal cancer cell lines. In several studies, miR-23b has been shown to play a role in the growth of a different cancer types (33)(34)(35)(36) and other miRNAs have been implicated in CSC biology (15,(37)(38)(39). Thus, we conjectured that miR-23b has a role in maintenance of the colonic CSC function.…”

Molecular Crosstalking among Noncoding RNAs: A New Network Layer of Genome Regulation in Cancer

“…Moreover, let-7a/b could indirectly suppress the β -catenin pathway, which in a different way could be activated by lncRNA FTHIP3 [111], but also is regulated by molecular axis miR-21-GAS5-miR-135b [112]. This unexpected crosstalking between ncRNA signaling could shed a light on expression relationships among ncRNAs and mRNAs, which have been frequently reported in cancer literature, but to date have not been satisfactorily explained [157–159]. This scenario is made more complex by the tissue-specific expression pattern of all ncRNAs, which could effectively influence the occurrence of specific interactions among ncRNAs.…”

Revisiting the thiosemicarbazonecopper(II) reaction with glutathione. Activity against colorectal carcinoma cell lines

“…Several studies have indicated that these mutations play distinct roles in development and therapy resistance of CRC [96]. Recently, the effects of the interaction of ribonucleotide reductases with thioredoxin [97] and the influence of the negatively regulated miRNA-mRNA pairs [98] and ribosome-inactivating stress [99] in CRC have been discussed. Taking into account that, as previously mentioned, both ribonucleotide reductases and RNA are targets of TSCs, and previous studies show the attack of CRC cells by TSCs [100,101], it could be interesting to apply these compounds to resistant CRC cells.…”