Identifying N sources that affect N uptake and assimilation in Vanda hybrid using 15N tracers

Panjama, Kanokwan; Ohyama, Takuji; Ohtake, Norikuni; Sato, Takashi; Potapohn, N.; Sueyoshi, Kuni; Ruamrungsri, Soraya

doi:10.1007/s13580-018-0071-6

Cited by 4 publications

(7 citation statements)

References 16 publications

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“…This suggests that Hippeastrum preferentially absorbs NO 3 − or mixed N forms rather than NH 4 + . Similar results were reported by Panjama et al (2018) who found that in Vanda, fertilizer solutions composed of combinations of 5 mM NO 3 − + 5 mM NH 4 + showed the highest 15 N use efficiency, followed by 10 mM NO 3 − , with the lowest N use efficiency in the 10 mM NH 4 + treatment. Rouphael et al (2016) suggested that nitrate-based fertilizer containing 14 mM NO 3 and 1 mM NH 4 is optimum for potted Hippeastrum due to higher WUEs and crop profitability.…”

Section: Effects Of N Source On Total Uptake Of Fertilizer N In Hippeastrumsupporting

confidence: 90%

“…Thus, leaves were a major organ for N accumulation when plants were supplied solely with NH 4 + as the N source. Panjama et al (2018) reported that in Vanda, the distribution of 15 N was higher in the leaves and slightly decreased in roots, regardless of the N source supplied, after 30 days of fertilizer treatment, clearly indicating that N absorbed in the roots was transported to the leaves. This result suggests that the leaves may be potential sink organs, especially young leaves, and have the highest sink strength during the vegetative growth phase of Vanda.…”

Section: Total N Content and Partitioning In Hippeastrum Organsmentioning

confidence: 95%

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Effect of N Source on Growth and N Uptake of Hippeastrum Using 15N Tracers

et al. 2022

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Understanding the mechanism of N uptake is of key importance to manage N utilization efficiency. Hippeastrum is a popular geophyte, but its N absorption and translocation characteristics are still not well understood. Therefore, the objective of this study was to assess the effect of different N sources on growth, N uptake and N distribution in Hippeastrum. The experiment was set up in a completely randomized design (CRD) with four different N sources of equal N concentration, i.e., 1) 2.5 mM 15 NO 3 − + 2.5 mM NH 4 + as treatment 1 (T1), 2) 2.5 mM NO 3 − + 2.5 mM 15 NH 4 + as treatment 2 (T2), 3) 5 mM 15 NO 3 − as treatment 3 (T3), and 4) 5 mM 15 NH 4 + as treatment 4 (T4). A 15 N-labelled 30 mL nutrient solution was drenched on plant pots (per time per pot). Plants were supplied with the 15 N solution every two weeks. Plant samples were divided into three stages according to plant growth stage, i.e., Stage 1 (emerging stage-1 WAP), Stage 2 (flowering stage-3 WAP), and Stage 3 (vegetative stage-15 WAP). The results indicated that plants fed with a combined N fertilizer and nitrate had a higher total dry weight than plants supplied solely with ammonium. At Stage 3, the plants supplied with 5 mM NO 3 − (T3) had a higher total N content than plants supplied with a mixed form of N (T1, T2) or solely NH 4 + (T4). In addition, plants supplied solely with NO 3 − (T3) had a higher N distribution in leaves than plants supplied with a mixed N source (T1, T2) and solely NH 4 + (T4). At 15 weeks after planting (Stage 3), a higher 15 N use efficiency ( 15 NUE) was observed in plants supplied with a mixed N source (T1, T2) and 5 mM 15 NO 3 − (T3) in comparison with those supplied solely with NH 4 + (T4). Scales were a major site of 15 N distribution in Hippeastrum at Stage 1. Nevertheless, most 15 N at Stage 2 and 3 was found in the roots + basal plate and leaves, respectively.

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Section: Effects Of N Source On Total Uptake Of Fertilizer N In Hippeastrumsupporting

confidence: 90%

Section: Total N Content and Partitioning In Hippeastrum Organsmentioning

confidence: 95%

Effect of N Source on Growth and N Uptake of Hippeastrum Using 15N Tracers

et al. 2022

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“…50-55%, and 40% was distributed in the roots. A similar result was reported by Panjama et al ( 2018) [19], in which Vanda 'Ratchaburi Fuchs-Katsura' had an N content in the leaves of about 49%, in the roots of 47%, and little was found in the stem. Vanda comprises many pairs of thickened leaves, and the young leaves are considered sink organs.…”

Section: Total N Content Distribution (%)supporting

confidence: 88%

“…15 N use efficiency ( 15 NUE) was calculated from the ratio between the amount of labeled N in the plant and the amount of labeled N supplied and expressed as a percentage [19]. 15…”

Section: Calculationsmentioning

confidence: 99%

Nitrogen Uptake and Translocation in Vanda Orchid after Roots and Leaves Application of Different Forms 15N Tracer

et al. 2022

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Vanda is an economically important orchid that is widely produced in Thailand. Usually, growers apply large amounts of fertilizer throughout the plant, covering the leaves and roots to ensure good quality products. Nitrogen fertilizer, in terms of ammonium (NH4+) and nitrate (NO3−), is generally used as an N source. In addition, nitrogen organic fertilizer (glutamine) is increasingly being used to promote rapid growth in some plants. However, the absorption efficiency of organic N compared with the inorganic form (NH4+ and NO3−) via the roots or leaves of Vanda has not been evaluated. Therefore, this research aimed to compare the fate of organic N (in glutamine form) and inorganic N in Vanda using a 15N tracer. Vanda ‘Patchara Delight’ was grown in a plastic greenhouse under a 50% shading net at an average temperature of 25 °C and 80% relative humidity (RH). The plants were sprayed weekly via roots or leaves with 100 mL of 15N solution, 2.5 mM 15NO3− + 2.5 mM NH4+ (N1), 2.5 mM NO3− + 2.5 mM 15NH4+ (N2), and 2.5 mM glutamine (15N2)(N3) for 4 weeks. The plants were then sampled and separated into leaves and roots, and 15N abundance was analyzed using an elemental analyzer coupled with an isotope-ratio mass spectrometer or IRMS. The plants that received only glutamine via roots showed the highest 15N use efficiency (15NUE) of about 28.19% at 30 days after the first feeding (DAF), whereas 15NH4+ resulted in the lowest 15NUE among 15N sources. Regardless of the application site, plants supplied with 15NH4+ showed a lower labeled N concentration and labeled N content in stems and leaves than those fed with a combination of 15NO3− or a sole application of 15N-glutamine. The largest labeled N concentrations in stems, leaves and roots were found in plants supplied with sole glutamine via roots. At 30 DAF, 15N solution either combined with 15NO3− or solitary 15N-glutamine did not affect the labeled N concentration in leaves. Therefore, supplying organic N in glutamine form to Vanda can provide a 4–7% higher NUE than inorganic N, especially when supplying the solution to the roots.

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“…Alanine distribution was high in leaves and roots at 7 days after feeding. Tyrosine distribution was predominant in leaves, while glutamine distribution was high in stems and roots at 30 days after fertilization [30].…”

Section: Tunicated Bulb Of Narcissus With Young Flower Inside (A) and Unbranched Roots (B) (Photo By Soraya Ruamrungsri)mentioning

confidence: 94%

Nitrogen in Flowers

Ruamrungsri¹,

Panjama²,

Ohyama³

et al. 2021

Nitrogen in Agriculture - Physiological, Agricultural and Ecological Aspects [Working Title]

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This chapter explores the literature and research on nitrogen in flowers. An overview of nitrogen deficiency symptoms in some flowers, i.e., Curcuma alismatifolia (ornamental curcuma), Tagetes erecta (marigold), Zinnia violacea (zinnia), and Gomphrena globose (gomphrena) were presented. Additionally, nitrogen uptake, translocation, and application in some flowers, i.e., ornamental curcuma, narcissus, orchids, and rose, were discussed in this chapter. Nitrogen affects the life cycle of flower, including vegetative and reproductive phases. Flower size, stem length, number of flowers per plant, and color were reduced by nitrogen deficiency. Therefore, the optimum level of nitrogen supply in each growth stage is important for flower crop production.

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Identifying N sources that affect N uptake and assimilation in Vanda hybrid using 15N tracers

Cited by 4 publications

References 16 publications

Effect of N Source on Growth and N Uptake of <i>Hippeastrum</i> Using <sup>15</sup>N Tracers

Effect of N Source on Growth and N Uptake of <i>Hippeastrum</i> Using <sup>15</sup>N Tracers

Nitrogen Uptake and Translocation in Vanda Orchid after Roots and Leaves Application of Different Forms 15N Tracer

Nitrogen in Flowers

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