Identifying potential biomarkers and therapeutic targets for dogs with sepsis using metabolomics and lipidomics analyses

Montague, Brett; Summers, April M.; Bhawal, Ruchika; Anderson, Elizabeth T.; Kraus-Malett, Sydney; Zhang, Sheng; Goggs, Robert

doi:10.1371/journal.pone.0271137

Cited by 11 publications

(4 citation statements)

References 130 publications

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“…A potential explanation for this finding is that histidine is mainly used in nonprotein turnover pathways like conversion to carnosine [27] and that these pathways are likely to be reduced in the critically ill. However, this explanation seems to be in contrast to the observed increased disposal into histidine breakdown pathways [28].…”

Section: Histidine Tryptophan and Methioninementioning

confidence: 77%

Amino acid kinetics in the critically ill

Deutz,

Haines,

Wischmeier

et al. 2023

Current Opinion in Clinical Nutrition &Amp; Metabolic Care

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Purpose of review Stable isotope methods have been used for many years to assess whole body protein and amino acid kinetics in critically ill patients. In recent years, new isotope approaches and tracer insights have been developed. The tracer pulse approach has some advantages above the established primed-continuous tracer infusion approach because of the high amount of metabolic information obtained, easy applicability, and low tracer costs. Effects of disease severity and sex on amino acid kinetics in ICU patients will also be addressed. Recent findings Current knowledge was synthesized on specific perturbations in amino acid metabolism in critically ill patients, employing novel methodologies such as the pulse tracer approach and computational modeling. Variations were evaluated in amino acid production and linked to severity of critical illness, as measured by SOFA score, and sex. Production of the branched-chain amino acids (BCAAs), glutamine, tau-methylhistidine and hydroxyproline were elevated in critical illness, likely related to increased transamination of the individual BCAAs or increased breakdown of proteins. Citrulline production was reduced, indicative of impaired gut mucosa function. Sex and disease severity independently influenced amino acid kinetics in ICU patients. Summary Novel tracer and computational approaches have been developed to simultaneously measure postabsorptive kinetics of multiple amino acids that can be used in critical illness. The collective findings lay the groundwork for targeted individualized nutritional strategies in ICU settings aimed at enhancing patient outcomes taking into account disease severity and sex.

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Section: Histidine Tryptophan and Methioninementioning

confidence: 77%

Amino acid kinetics in the critically ill

Deutz,

Haines,

Wischmeier

et al. 2023

Current Opinion in Clinical Nutrition &Amp; Metabolic Care

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“…Additionally, the metabolite significance plot (Figure 6B) highlighted 13,14-dihydro-15-keto prostaglandin A2, pregnanetriol, alpha-farnesene, heptadecanoic acid, L-adrenaline, α-lapachone, and stercobilin as metabolites with VIP > 1 and p < 0.05. Notably, 13,14-Dihydro-15-keto prostaglandin A2, which has high discriminatory power, is a potential biomarker for sepsis, associated with increased morbidity and mortality risk [54]. Similarly, alpha-farnesene, the main alarm pheromone, has potential applications as a protective agent in agriculture [55].…”

Section: Discussionmentioning

confidence: 99%

Regulatory Effects of Ganoderma lucidum, Grifola frondosa, and American ginseng Extract Formulation on Gut Microbiota and Fecal Metabolomics in Mice

Zhang,

Huang,

Zhao

2023

Foods

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The bioactivities of Ganoderma lucidum, Grifola frondosa, and American ginseng have been extensively studied and documented. However, the effects of their complexes on the structural properties of intestinal microbiota and fecal metabolism remain unclear. Therefore, this paper aims to present a preliminary study to shed light on this aspect. In this study, an immunocompromised mouse model was induced using cyclophosphamide, and Ganoderma lucidum, Grifola frondosa, and American ginseng extract formulation (referred to as JGGA) were administered via gavage to investigate their modulatory effects on gut microbiota and fecal metabolism in mice. The effects of JGGA on immune enhancement were explored using serum test kits, hematoxylin–eosin staining, 16SrDNA high-throughput sequencing, and UHPLC-QE-MS metabolomics. The findings revealed potential mechanisms underlying the immune-enhancing effects of JGGA. Specifically, JGGA administration resulted in an improved body weight, thymic index, splenic index, carbon scavenging ability, hypersensitivity, and cellular inflammatory factor expression levels in mice. Further analysis demonstrated that JGGA reduced the abundance of Firmicutes, Proteobacteria, and Actinobacteria, while increasing the abundance of Bacteroidetes. Additionally, JGGA modulated the levels of 30 fecal metabolites. These results suggest that the immune enhancement observed with JGGA may be attributed to the targeted modulation of gut microbiota and fecal metabolism, thus promoting increased immunity in the body.

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“…In particular, neoplastic diseases investigated through omics technologies are hematopoietic neoplasms [79][80][81][82], mammary tumors [83][84][85], oral neoplasms [86], neuroendocrine tumors [87], and melanocytic tumors [73,88]. Further disorders addressed by omics approaches include cardiopathies [89][90][91], endocrinopathies such as hyperadrenocorticism [92] and diabetes mellitus [93,94], hepatopathies [95][96][97], chronic kidney disease [98,99], and inflammatory/infectious diseases(i.e., sepsis [100], pyometra [101], and vector-borne diseases [102,103]). Interestingly, using omics approaches, great attention has also been paid to the gut microbiota [104,105] and dietary regimens [106,107].…”

Section: Integrative Multiomics Approaches In Veterinary Medicinementioning

confidence: 99%

Omics Technologies in Veterinary Medicine: Literature Review and Perspectives in Transfusion Medicine

Miglio,

Cremonini,

Leonardi

et al. 2023

Transfus Med Hemother

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Background: Omics technologies represent a new analytical approach that allows a full cellular readout through the simultaneous analysis of thousands of molecules. The application of such technologies represents a flourishing field of research in human medicine, especially in transfusion medicine, while their application in veterinary medicine still needs to be developed. Summary: Omics technologies, especially proteomics, metabolomics, and lipidomics, are currently applied in several fields of human medicine. In transfusion medicine, the creation and integration of multiomics datasets have uncovered intricate molecular pathways occurring within blood bags during storage. In particular, the research has been directed toward the study of storage lesions (SLs), i.e., those biochemical and structural changes that red blood cells (RBCs) undergo during hypothermic storage, their causes, and the development of new strategies to prevent them. However, due to their challenges to perform and high costs, these technologies are hardly accessible to veterinary research, where their application dates back only to the last few years and thus a great deal of progress still needs to be made. As regards veterinary medicine, there are only a few studies that have focused mainly on fields such as oncology, nutrition, cardiology, and nephrology. Other studies have suggested omics datasets that provide important insights for future comparative investigations between human and nonhuman species. Regarding the study of storage lesions and, more generally, the veterinary transfusion field, there is a marked lack of available omics data and results with relevance for clinical practice. Key Messages: The use of omics technologies in human medicine is well established and has led to promising results in blood transfusion and related practices knowledge. Transfusion practice is a burgeoning field in veterinary medicine, but, to date, there are no species-specific procedures and techniques for the collection and storage of blood units and those validated in the human species are univocally pursued. Multiomics analysis of the species-specific RBCs’ biological characteristics could provide promising results both from a comparative perspective, by increasing our understanding of species suitable to be used as animal models, and in a strictly veterinary view, by contributing to the development of animal-targeted procedures.

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Identifying potential biomarkers and therapeutic targets for dogs with sepsis using metabolomics and lipidomics analyses

Cited by 11 publications

References 130 publications

Amino acid kinetics in the critically ill

Amino acid kinetics in the critically ill

Regulatory Effects of Ganoderma lucidum, Grifola frondosa, and American ginseng Extract Formulation on Gut Microbiota and Fecal Metabolomics in Mice

Omics Technologies in Veterinary Medicine: Literature Review and Perspectives in Transfusion Medicine

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