2019
DOI: 10.1038/s41598-019-47746-9
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Identifying potential novel drugs against Helicobacter pylori by targeting the essential response regulator HsrA

Abstract: The increasing antibiotic resistance evolved by Helicobacter pylori has alarmingly reduced the eradication rates of first-line therapies. To overcome the current circulating resistome, we selected a novel potential therapeutic target in order to identify new candidate drugs for treating H . pylori infection. We screened 1120 FDA-approved drugs for molecules that bind to the essential response regulator HsrA and potentially inhibit its biologi… Show more

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Cited by 45 publications
(91 citation statements)
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“…EMSA experiments were performed as previously described [16] with slight modifications. Briefly, a 300-bp promoter region of the H. pylori rocF (arginase) gene was amplified by PCR and used as target DNA sequence of ArsR [6] in all EMSA experiments.…”
Section: Electrophoretic Mobility Shift Assaysmentioning
confidence: 99%
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“…EMSA experiments were performed as previously described [16] with slight modifications. Briefly, a 300-bp promoter region of the H. pylori rocF (arginase) gene was amplified by PCR and used as target DNA sequence of ArsR [6] in all EMSA experiments.…”
Section: Electrophoretic Mobility Shift Assaysmentioning
confidence: 99%
“…Inhibition studies of the HsrA activity by selected ArsR binders were performed as previously described [16,17]. Briefly, 120 ng of H. pylori porGDAB promoter were mixed with 6 µM of recombinant HsrA in the presence of 1 mM of each ArsR binders.…”
Section: Electrophoretic Mobility Shift Assaysmentioning
confidence: 99%
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