Identifying the Antitumor Effects of Curcumin on Lung Adenocarcinoma Using Comprehensive Bioinformatics Analysis

Abstract

Background As the main component of turmeric ( Curcuma longa L .), curcumin is widely used in the treatment of various diseases. Previous studies have demonstrated that curcumin has great potential as a therapeutic agent, but the lack of understanding of the functional mechanism of the drug has hindered the widespread use of the natural product. In the present study, we used comprehensive bioinformatics analysis and in vitro experiments to explore the anti-tumor mechanism of curcumin. Materials and Methods LUAD mRNA expression data were obtained from TCGA database and differentially expressed genes (DEGs) were identified using R software. Functional enrichment analysis was conducted to further clarify its biological properties and hub genes were identified by a protein–protein interaction (PPI) network analysis. Survival analysis and molecular docking were used to analyze the effectiveness of the hub genes. By an in vitro study, we evaluated whether curcumin could influence the proliferation, migration, and invasion activities of LUAD cells. Results In this study, 1783 DEGs from LUAD tissue samples compared to normal samples were evaluated. Functional enrichment analysis and the PPI network revealed the characteristics of the DEGs. We performed a topological analysis and identified 10 hub genes. Of these, six genes ( INS, GCG, SST, F2, AHSG, and NPY ) were identified as potentially effective biomarkers of LUAD. The molecular docking results indicated that curcumin targets in regulating lung cancer may be INS and GCG . We found that curcumin significantly inhibited the proliferation, migration, and invasion of LUAD cells and significantly decreased the expression of the INS and GCG genes. Conclusion The results of this study suggest that the therapeutic effects of curcumin on LUAD may be achieved through the intervention of INS and GCG , which may act as potential biomarkers for LUAD prevention and treatment.

Identification of a coagulation‐related signature correlated with immune infiltration and their prognostic implications in lung adenocarcinoma

“…F2 is known as coagulation factor. The high expression of F2 is associated with the bad prognosis in LUAD 33 . The expression of PLAUR correlated with the hallmark gene set “TNFα‐signaling via NFκB”, which has a direct pro‐coagulant effect.…”

“…The high expression of F2 is associated with the bad prognosis in LUAD. 33 The expression of PLAUR correlated with the hallmark gene set "TNFαsignaling via NFκB", which has a direct pro-coagulant effect. Upon exposure to TNFα, cancer cells increase their expression of TF and produce TF-bearing microparticles with potent local pro-coagulant effects.…”

Anticancer and Antibacterial Properties of Curcumin-Loaded Mannosylated Solid Lipid Nanoparticles for the Treatment of Lung Diseases

“…Despite successful surgical resection during early disease stages, the prognosis remains poor, with a substantial proportion of patients presenting with local or distant recurrence and a 5-year survival rate of less than 15% . Accordingly, it is required to develop new and effective therapeutic drugs and agents to treat lung cancer. , To overcome the limitations of conventional anticancer drugs, novel therapies that are less toxic and can selectively target the disease have been extensively explored. Plant-derived natural products have gained considerable attention as ideal sources for novel therapeutics with limited toxicity owing to their structural diversity and ability to target diverse growth pathways in cancer cells .…”