This research attempted to identify the knowledge in self-care practices of patients receiving dialysis with arteriovenous fistula and the effect of an instructional module that consequently improve their self-care practices. The experimental design included a sample of 71 patients using AV fistula to perform hemodialysis at dialysis unit of King Khaled Hospital and Hail Dialysis care center, Hail region, Saudi Arabia. Results showed an increase in mean score on general instructions from 1.78 ± 0.33 in pre-test to 2.78 ± 0.22 in post-test and also the mean score of knowledge about look for infection from 2.04 ± 0.51 in pre-test to 2.33 ± 0.31 in post-test has observed. Moreover the increase of the mean score of knowledge related to the assessment of AVF function from 2.0 ± 0.38 in pre-test to 2.7 ± 0.29 in posttest with statistically significance improvement p < 0.5. The written instructional module with explanation enhanced better understanding and promoted the self-care practices among patients using AV fistula.