Identifying three different architectural subtypes of mammary ductal carcinoma<i>in situ</i>using multiphoton microscopy

Wu, Yan; Fu, Fangmeng; Lian, Yuane; Nie, Yuting; Zhuo, Shuangmu; Wang, Chuan; Chen, Jianxin

doi:10.1088/0022-3727/48/40/405401

Cited by 9 publications

(10 citation statements)

References 32 publications

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“…Moreover, MPM also plays an important role in breast cancer research, including rapid intraoperative evaluation of breast lesions, detection of breast tumor progression, and the role of collagen density in the mammary tumor initiation and progression [15,25,26]. However, few MPM studies have been conducted on these early stages of breast ductal carcinoma [16], especially on MPM imaging of ADH and DCIS-MI. In recent years, these early stages of breast ductal carcinoma have attracted more and more scholars' attention because of the good prognosis of these lesions after treatment [27].…”

Section: Discussionmentioning

confidence: 99%

“…Multiphoton microscopy (MPM) via two-photon excited fluorescence (TPEF) combined with second-harmonic generation (SHG) is a promising, label-free, and high-resolution imaging technique for cancer research and diagnosis [12][13][14][15]. There are abundant autofluorescent substances in breast tissue, such as elastic fibers, NAD(P)H, and FAD, which can generate TPEF signals [16,17]; asymmetric collagen such as type I collagen, type II collagen, type III collagen, and type V collagen is more likely to produce SHG signals [18]. Particularly, SHG imaging visualizes the microstructure of collagen fibers and elastin fibers in the extracellular matrix by detecting tissue intrinsic fluorescence [19].…”

Section: Introductionmentioning

confidence: 99%

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Label-Free Identification of Early Stages of Breast Ductal Carcinoma via Multiphoton Microscopy

et al. 2020

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Breast cancer can be cured by early diagnosis. Appropriate and effective clinical treatment benefits from accurate pathological diagnosis. However, due to the lack of effective screening and diagnostic imaging methods, early stages of breast cancer often progress to malignant breast cancer. In this study, multiphoton microscopy (MPM) via two-photon excited fluorescence combined with second-harmonic generation was used for identifying the early stages of breast ductal carcinoma. The results showed differences in both cytological features and collagen distribution among normal breast tissue, atypical ductal hyperplasia, low-grade ductal carcinoma in situ, and high-grade ductal carcinoma in situ with microinvasion. Furthermore, three features extracted from the MPM images were used to describe differences in cytological features, collagen density, and basement membrane circumference in the early stages of breast ductal carcinoma. They revealed that MPM has the ability to identify early stages of breast ductal carcinoma label-free, which would contribute to the early diagnosis and treatment of breast cancer. This study may provide the groundwork for the further application of MPM in the clinic.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Label-Free Identification of Early Stages of Breast Ductal Carcinoma via Multiphoton Microscopy

et al. 2020

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“…This study was approved by the Institutional Review Board of the Affiliated Union Hospital of Fujian Medical University (Fuzhou, China) and informed consent was obtained from each patient who participated. We followed the methods of Wu et al 2015 [ 1 ]. A total of 12 samples (6 fibroadenomas and 6 fibrocystic diseases) from 12 different patients were collected after resection from patients.…”

Section: Methodsmentioning

confidence: 99%

“…Multiphoton microscopy (MPM) has opened up the new possibilities for in vivo imaging biological tissues at the cellular and subcellular levels with high spatial resolution, by taking advantage of the natural fluorescing and optical properties of intrinsic biomolecules [ 1 – 3 ]. It displays several advantages over traditional imaging technique, such as being label-free, deep tissue imaging, and low photobleaching and phototoxicity, based on two-photon excited fluorescence (TPEF) and second-harmonic generation (SHG) [ 4 – 6 ].…”

Section: Introductionmentioning

confidence: 99%

Identifying Two Common Types of Breast Benign Diseases Based on Multiphoton Microscopy

Lin

Lian

et al. 2018

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Multiphoton microscopy has attracted increasing attention and investigations in the field of breast cancer, based on two-photon excited fluorescence (TPEF) and second-harmonic generation (SHG). However, the incidence of breast benign diseases is about 5 to 10 times higher than breast cancer; up to 30% of women suffer from breast benign diseases and require treatment at some time in their lives. Thus, in this study, MPM was applied to image fibroadenoma and fibrocystic lesion, which are two of the most common breast benign diseases. The results show that MPM has the capability to identify the microstructure of lobule and stroma in normal breast tissue, the interaction of compressed ducts with surrounding collagen fiber in fibroadenoma, and the architecture of cysts filled with cystic fluid in fibrocystic disease. These findings indicate that, with integration of MPM into currently accepted clinical imaging system, it has the potential to make a real-time diagnosis of breast benign diseases in vivo, as well as breast cancer.

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“…MPM can not only identify DCIS, but also can make accurate judgments on the pathological type of DCIS. Wu et al used MPM to identify three different pathological subtypes of DCIS: solid, cribriform, and comedo, as shown in Figure 4 93 . From the MPM images, the detailed structure of ductal carcinoma in situ could be seen.…”

Section: Monitoring Breast Disease Progression By Mpmmentioning

confidence: 99%

Multiphoton microscopy for monitoring the occurrence, metastasis and therapy of breast cancer

Liu

et al. 2021

Micro & Optical Tech Letters

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Worldwide, the most common malignancy in women is breast cancer. At present, the gold standard for breast cancer diagnosis, namely pathological analysis, cannot reach the subcellular level, and is unable to provide more physiological information. Multiphoton microscopy (MPM) has its unique advantages, such as label‐free detection, high resolution, low damage, deep imaging depth, low photobleaching and phototoxicity, and so on, which make up for the deficiences of traditional methods. This review mainly introduces the principle of MPM and its applications in breast diseases, especially in monitoring the occurrence, progression, metastasis, and therapy response of tumor. At the same time, the performance and progress of MPM combined with nanomaterials in breast cancer treatment are summarized. Finally, we propose the clinical challenges and future research directions of MPM.

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Identifying three different architectural subtypes of mammary ductal carcinomain situusing multiphoton microscopy

Cited by 9 publications

References 32 publications

Label-Free Identification of Early Stages of Breast Ductal Carcinoma via Multiphoton Microscopy

Label-Free Identification of Early Stages of Breast Ductal Carcinoma via Multiphoton Microscopy

Identifying Two Common Types of Breast Benign Diseases Based on Multiphoton Microscopy

Multiphoton microscopy for monitoring the occurrence, metastasis and therapy of breast cancer

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