Identifying tumor antigens and immune subtypes of renal cell carcinoma for immunotherapy development

Chen, Xinglin; Zhang, Tongtong; Zhai, Xinyu; Zhong, Wu; Ge, Minyao; Liu, Chengzong; Tan, Mingyue; Xu, Dongliang

doi:10.3389/fimmu.2022.1037808

Cited by 2 publications

(2 citation statements)

References 26 publications

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“…The CCK8 assay was conducted using the CCK8 kit (DAKEWEI) as described previously ( 18 ). In brief, 100 μL of DC2.4 cell suspension (1×10 6 /mL) was prepared in a 96-well plate.…”

Section: Methodsmentioning

confidence: 99%

Self-assembled ferritin nanoparticles displaying PcrV and OprI as an adjuvant-free Pseudomonas aeruginosa vaccine

Li,

Pu,

Zhang

et al. 2023

Front. Immunol.

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IntroductionSerious infections of Pseudomonas aeruginosa (PA) in hospitals and the emergence and increase of multidrug resistance have raised an urgent need for effective vaccines. However, no vaccine has been approved to date. One possible reason for this is the limited immune response due to the lack of an efficient delivery system. Self-assembled ferritin nanoparticles are good carriers of heterogeneous antigens, which enhance the activation of immunological responses.MethodsIn this study, two well-studied antigen candidates, PcrV and OprI, were selected and connected to the ferritin nanoparticle by the Spytag/SpyCatcher system to generate the nanovaccine rePO-FN.ResultsCompared to recombinant PcrV-OprI formulated with aluminum adjuvants, intramuscular immunization with adjuvant-free rePO-FN induced quick and efficient immunity and conferred protection against PA pneumonia in mice. In addition, intranasal immunization with adjuvant-free rePO-FN enhanced protective mucosal immunity. Moreover, rePO-FN exhibited good biocompatibility and safety.DiscussionOur results suggest that rePO-FN is a promising vaccine candidate, as well as, provide additional evidence for the success of ferritin-based nanovaccines.

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“…The CCK8 assay was conducted using the CCK8 kit (DAKEWEI) as described previously ( 18 ). In brief, 100 μL of DC2.4 cell suspension (1×10 6 /mL) was prepared in a 96-well plate.…”

Section: Methodsmentioning

confidence: 99%

Self-assembled ferritin nanoparticles displaying PcrV and OprI as an adjuvant-free Pseudomonas aeruginosa vaccine

Li,

Pu,

Zhang

et al. 2023

Front. Immunol.

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show abstract

“…When the cell density reached approximately 30%-40% and good growth and even distribution were observed, predesigned siRNA and overexpression plasmids (Jima Biotechnology Co., Ltd., Shanghai, China) were transfected into the cells using Lipofectamine 2000 Reagent (Thermo Fisher Scienti c Inc. MA, USA.) following previously described methods [40].HT-22 cells were assigned to groups as described in Section 2.3 after overexpression or knockdown of Sp1 and GlyT1(siRNA primers sequences shown in Table 2), and the cells were further incubated in an incubator until subsequent experimental observation was performed.…”

Section: Cell Transfectionmentioning

confidence: 99%

NEDD4L-Sp1 ubiquitination inhibits GlyT1 to promote prominent hippocampal neuronal damage and apoptosis, leading to cognitive dysfunction in diabetic rats

Yang

Zhū

et al. 2023

Preprint

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Diabetes-associated cognitive dysfunction(DACD) is one of the neurological complications of diabetes, and it mainly involves the hippocampal region of the brain and affects the learning and memory functions of the body. There are many studies on the pathogenesis of DACD, but there is a lack of in-depth studies on the underlying molecular mechanism, which poses a great challenge to drug development. In this study, we focused on the molecular mechanism by which signal transduction by the glycine transporter GlyT1 participates in the development of DACD and systematically elucidated the processes of synaptic plasticity and apoptosis in hippocampal neurons. The results showed that when neurons were exposed to a high-glucose environment, low levels of GlyT1 inhibited the activation of the PI3K/AKT/mTOR pathway to promote neuronal apoptosis; additionally, GlyT1 regulated NMDR expression to regulate glycine concentrations in order to reduce synaptic plasticity. The transcription factor Sp1 bound to the GlyT1 promoter region and regulated GlyT1 expression, so we explored whether Sp1 expression was regulated by the protease-ubiquitin system, resulting in decreased Sp1 levels.In conclusion, In conclusion, our study systematically demonstrated the biological function and molecular mechanism by which GlyT1 participates in DACD development, elucidated the upstream and downstream mechanisms of GlyT1 regulation, provided reliable molecular targets for DACD treatment, and enhanced the understanding of the mechanism underlying DACD development.

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Identifying tumor antigens and immune subtypes of renal cell carcinoma for immunotherapy development

Cited by 2 publications

References 26 publications

Self-assembled ferritin nanoparticles displaying PcrV and OprI as an adjuvant-free Pseudomonas aeruginosa vaccine

Self-assembled ferritin nanoparticles displaying PcrV and OprI as an adjuvant-free Pseudomonas aeruginosa vaccine

NEDD4L-Sp1 ubiquitination inhibits GlyT1 to promote prominent hippocampal neuronal damage and apoptosis, leading to cognitive dysfunction in diabetic rats

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