Identifying useable semen

Foxcroft, G. R.; Dyck, Michael K.; Sánchez, Ana Ruíz; Novak, Susan; Dixon, Walter T.

doi:10.1016/j.theriogenology.2008.07.015

Cited by 64 publications

(60 citation statements)

References 84 publications

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“…Although we have previously speculated that this protein may be lipocalin-type prostaglandin D synthase (Foxcroft et al, 2008), this study confirmed its identity using MS as glutathione peroxidase-5. Glutathione peroxidase-5 is a seleniumindependent free radical scavenger that is restricted to the male reproductive tract (Grignard et al, 2005).…”

Section: Discussionsupporting

confidence: 67%

“…As a part of the fertilization process, seminal plasma proteins play an important role in sperm reservoir formation, sperm capacitation, and sperm-oocyte interactions (reviewed by Foxcroft et al, 2008;Rodríguez-Martínez et al, 2008). Specific seminal plasma proteins have previously been identified as potential markers of male fertility in the bull (Killian et al, 1993) and stallion (Brandon et al, 1999).…”

Section: Discussionmentioning

confidence: 99%

“…For artificial insemination purposes, either the first sperm-rich fraction or the whole ejaculate may be collected; however, in a natural mating scenario, the sperm would typically only come in contact with the sperm-rich fraction of the ejaculate (Foxcroft et al, 2008). Thus, it may become important when collecting semen to collect only the first sperm-rich fraction rather than the whole ejaculate, especially if the second fraction of the ejaculate may contain components that are inhibitory to overall boar fertility.…”

Section: Discussionmentioning

confidence: 99%

“…Physical semen characteristics and sperm morphology measurements are not always indicative of fertility and reproductive performance in boars, and accurate and predictive genetic and protein markers are still needed (as reviewed by Foxcroft et al, 2008). Specific proteins in seminal plasma have been identified as potential markers of male fertility or infertility in the human (Yamakawa et al, 2007;Martínez-Heredia et al, 2008).…”

mentioning

confidence: 99%

“…Seminal plasma is composed of secretions from the male accessory sex glands and epididymis, which contains many organic and inorganic components that have effects on sperm quality (Foxcroft et al, 2008). The proteins secreted into seminal plasma may play an important role during sperm capacitation and fertilization (Rodríguez-Martínez et al, 1998) and may also serve to protect sperm from damage or to maintain their longevity.…”

mentioning

confidence: 99%

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Seminal Plasma Proteins as Potential Markers of Relative Fertility in Boars

Novak

Sánchez

Dixon

et al. 2010

Journal of Andrology

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This study investigated whether specific proteins from distinct seminal plasma fractions of boars could be related to in vivo fertility. Nine boars with acceptable sperm motility and morphology for use in artificial insemination demonstrated major differences in total number born and pregnancy rate when low sperm doses (1.5 billion sperm) were used to breed a minimum of 50 gilts per boar. The 2 lowest-fertility and 2 highest-fertility boars were chosen for evaluation of specific seminal plasma proteins. On 4 occasions, semen was collected and separated into 3 fractions based on sperm concentration (Sperm-Peak, Sperm-Rich, and Sperm-Free), and the fractions were analyzed for total protein concentration and abundance of major seminal plasma glycoprotein (PSP-I), AWN-1, and osteopontin protein using Western blotting techniques. The concentrations of these seminal plasma proteins were lower in the Sperm-Peak fractions compared with the SpermFree fractions (P , .05). Seminal plasma from the pooled SpermRich fraction used for artificial insemination was also subjected to two-dimensional gel electrophoresis to investigate novel protein markers related to in vivo fertility. Total piglets born (r 5 20.76, P 5 .01) and sperm motility at day 7 (r 5 20.74, P 5 .037) were again negatively correlated with a 22-kDa protein identified by mass spectrometry as PSP-I. However, fertility index and farrowing rate tended to be positively correlated (P , .10) with a 25-kDa protein, identified as glutathione peroxidase (GPX5), an antioxidant enzyme that may protect sperm membranes from oxidative damage. These candidate proteins merit further investigation as markers of fertility in boars.

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Section: Discussionsupporting

confidence: 67%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Seminal Plasma Proteins as Potential Markers of Relative Fertility in Boars

Novak

Sánchez

Dixon

et al. 2010

Journal of Andrology

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Correlation between human clusterin in seminal plasma with sperm protamine deficiency and DNA fragmentation

Salehi

Akbari

Heidari

et al. 2013

Molecular Reproduction Devel

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Seminal proteins can be considered as factors that control fertilization. Clusterin is one such protein that has been implicated in many activities, including apoptosis inhibition, cell cycle control, DNA repair, and sperm maturation. In this study, the relationship between human secretory clusterin (sCLU) in seminal plasma with sperm parameters, protamine deficiency, and DNA fragmentation was investigated. Semen samples were collected from 63 Iranian men, and semen analysis was performed according to World Health Organization criteria and computer aided semen analysis (CASA). The concentration of sCLU in seminal plasma was measured by enzyme-linked immunosorbant assay (ELISA), protamine deficiency was determined by chromomycin A3 staining (CMA3 ), and sperm DNA fragmentation was checked by sperm chromatin dispersion (SCD) assay. The level of sCLU in seminal fluid of fertile patients was 48.3 ± 38.6 ng/ml and in infertile patients was 15.5 ± 9.7 ng/ml; this difference was significant (P < 0.001). sCLU correlated negatively with protamine deficiency, sperm DNA fragmentation, and abnormal morphology. In conclusion, seminal clusterin can be considered as a marker for the quick assessment of semen quality in male infertility studies.

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RFamide‐related peptide 3 and gonadotropin‐releasing hormone‐II are autocrine–paracrine regulators of testicular function in the boar

Lents

Thorson

Desaulniers

et al. 2017

Molecular Reproduction Devel

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Widespread use of artificial insemination in swine requires millions of doses of boar semen each year. Subfertility of boars remains a major constraint, which can impact the reproductive efficiency of thousands of sows, so a better understanding of testicular function is needed in order to develop methods to improve semen production. With this in mind, the effects of RFamide-related peptide 3 (RFRP3) and Gonadotropin-releasing hormone-II (GnRH-II) on gonadotropin secretion and testicular function of pigs are reviewed here. Receptors for RFRP3 are present in the pig hypothalamus, adenohypophysis, and testis. Evidence from in vitro studies indicates that RFRP3 could be a hypophysiotropic hormone in the pig by suppressing secretion of GnRH-I from the hypothalamus and luteinizing hormone (LH) from the pituitary gland; however, effects of RFRP3 on in vivo secretion of LH in pigs are minimal. Within the pig testis, RFRP3 suppresses testosterone secretion by inhibiting steroidogenic enzymes. GnRH-II and its receptor (GnRHR-II) are abundant in pig testes. Interstitial cells express GnRHR-II, and exogenous GnRH-II robustly stimulates secretion of testosterone in boars, despite minimal secretion of LH. Data illustrate that GnRH-II directly stimulates secretion of testosterone from the testes of mature boars. Thus, the primary function of RFRP3 and GnRH-II in the boar appears to be autocrine-paracrine inhibition and stimulation, respectively, of testosterone secretion within the testis. A better understanding of changes in the RFRP3 and GnRH-II systems within the testis during development will provide important clues about how to improve the testicular function of boars.

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Identifying useable semen

Cited by 64 publications

References 84 publications

Seminal Plasma Proteins as Potential Markers of Relative Fertility in Boars

Seminal Plasma Proteins as Potential Markers of Relative Fertility in Boars

Correlation between human clusterin in seminal plasma with sperm protamine deficiency and DNA fragmentation

RFamide‐related peptide 3 and gonadotropin‐releasing hormone‐II are autocrine–paracrine regulators of testicular function in the boar

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