Identifying Verticillium dahliae Resistance in Strawberry Through Disease Screening of Multiple Populations and Image Based Phenotyping

Cockerton, Helen M.; Li, Bo; Vickerstaff, Robert J.; Eyre, Catherine A.; Armitage, Andrew D.; Marina-Montes, César; Garcia-Cruz, Ana; Passey, A. J.; Simpson, D. W.; Harrison, Richard J.

doi:10.3389/fpls.2019.00924

Cited by 26 publications

(19 citation statements)

References 69 publications

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“…The simple (gene‐for‐gene) solution to the problem of developing resistant cultivars has not been feasible in strawberry because R ‐genes conferring resistance to Verticillium wilt have not been identified (Maas, Galletta, & Draper, 1989; Vining, Davis, Jamieson, & Mahoney, 2015). Large‐effect quantitative trait loci (QTL) have not been identified either (Antanaviciute et al., 2015; Cockerton et al., 2019). Consequently, phenotypic selection for quantitative resistance to this pathogen has previously been the only solution (Bringhurst, Hansche, & Voth, 1968; Darrow, 1966; Maas et al., 1989; Shaw, Gubler, Larson, & Hansen, 1996, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…With recent advances in the development of genomic resources and supporting information (Edger et al., 2019; Hardigan et al., 2020; Whitaker et al., 2020), strawberry is well positioned for the application of genome‐informed breeding approaches (Crossa et al., 2017; Poland & Rutkoski, 2016). Here, we explore the prospects for increasing genetic gains and accelerating the development of Verticillium wilt resistant cultivars through the application of genomic selection, under the assumption that resistance to this pathogen is genetically complex in cultivated strawberry (Antanaviciute et al., 2015; Cockerton et al., 2019; Shaw et al., 1996; Vining et al., 2015).…”

Section: Introductionmentioning

confidence: 99%

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Accuracy of genomic selection and long‐term genetic gain for resistance to Verticillium wilt in strawberry

et al. 2020

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Verticillium wilt, a soil-borne disease caused by the fungal pathogen Verticillium dahliae, threatens strawberry (Fragaria × ananassa) production worldwide. The development of resistant cultivars has been a persistent challenge, in part because the genetics of resistance is complex. The heritability of resistance and genetic gains in breeding for resistance to this pathogen have not been well documented. To elucidate the genetics, assess long-term genetic gains, and estimate the accuracy of genomic selection for resistance to Verticillium wilt, we analyzed a genetically diverse population of elite and exotic germplasm accessions (n = 984), including 245 cultivars developed since 1854. We observed a full range of phenotypes, from highly susceptible to highly resistant: < 3% were classified as highly resistant, whereas > 50% were classified as moderately to highly susceptible. Broad-sense heritability estimates ranged from 0.70-0.76, whereas narrow-sense genomic heritability estimates ranged from 0.33-0.45. We found that genetic gains in breeding for resistance to Verticillium wilt have been negative over the last 165 years (mean resistance has decreased over time). We identified several highly resistant accessions that might harbor favorable alleles that are either rare or non-existent in modern populations. We did not observe the segregation of large-effect loci. The accuracy of genomic predictions ranged from 0.38-0.53 among years and whole-genome regression methods. We show that genomic selection has promise for increasing genetic gains and accelerating the development of resistant cultivars in strawberry by shortening selection cycles and enabling selection in early developmental stages without phenotyping.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Accuracy of genomic selection and long‐term genetic gain for resistance to Verticillium wilt in strawberry

et al. 2020

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“…Similar resistance responses have been observed within and outside the Solanaceae family for various Ve1 homologs [ 64 ]. However, several studies have reported that VW resistance is controlled by additive quantitative genetic components located in multiple small effect genomic loci (quantitative trait locus, QTLs) [ 65 , 66 , 67 , 68 , 69 , 70 , 71 ]. Among these, the QTL for V. longisporum resistance in oilseed rape co-localized with QTLs for a number of phenylpropanoids [ 72 ].…”

Section: Discussionmentioning

confidence: 99%

Hop Polyphenols in Relation to Verticillium Wilt Resistance and Their Antifungal Activity

Berne

Kovačević

Kastelec

et al. 2020

Plants

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(1) Background: Verticillium wilt (VW) of hop is a devastating disease caused by the soil-borne fungi Verticillium nonalfalfae and Verticillium dahliae. As suggested by quantitative trait locus (QTL) mapping and RNA-Seq analyses, the underlying molecular mechanisms of resistance in hop are complex, consisting of preformed and induced defense responses, including the synthesis of various phenolic compounds. (2) Methods: We determined the total polyphenolic content at two phenological stages in roots and stems of 14 hop varieties differing in VW resistance, examined the changes in the total polyphenols of VW resistant variety Wye Target (WT) and susceptible Celeia (CE) on infection with V. nonalfalfae, and assessed the antifungal activity of six commercial phenolic compounds and total polyphenolic extracts from roots and stems of VW resistant WT and susceptible CE on the growth of two different V. nonalfalfae hop pathotypes. (3) Results: Generally, total polyphenols were higher in roots than stems and increased with maturation of the hop. Before flowering, the majority of VW resistant varieties had a significantly higher content of total polyphenols in stems than susceptible varieties. At the symptomatic stage of VW disease, total polyphenols decreased in VW resistant WT and susceptible CE plants in both roots and stems. The antifungal activity of total polyphenolic extracts against V. nonalfalfae was higher in hop extracts from stems than those from roots. Among the tested phenolic compounds, only p-coumaric acid and tyrosol markedly restricted fungal growth. (4) Conclusions: Although the correlation between VW resistance and total polyphenols content is not straightforward, higher levels of total polyphenols in the stems of the majority of VW resistant hop varieties at early phenological stages probably contribute to fast and efficient activation of signaling pathways, leading to successful defense against V. nonalfalfae infection.

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“…The strawberry (Fragaria x ananassa) 'Redgauntlet' x 'Hapil' mapping population was used to study root architecture, AMF association and low phosphate tolerance. Plant material was generated at NIAB EMR, the F1 cross was initially made to study Verticillium dahliae resistance [48]. For the initial assessment of parental root architecture, 10 runner plants of 'Redgauntlet' and 'Hapil' were pinned down into rhizotrons.…”

Section: Root Architecture Quantification Plant Materialsmentioning

confidence: 99%

“…QTL identified in both analysis are considered to be robust and thus reported here. Heritability and proportional reduction of error was calculated as specified in Cockerton et al, 2019 [48]. Principal component analysis was used to determine the components accounting for the largest proportion of variation in genotypes.…”

Section: Quantitative Trait Loci (Qtl) Analysismentioning

confidence: 99%

Genetic and phenotypic associations between root architecture, arbuscular mycorrhizal fungi colonisation and low phosphate tolerance in strawberry (Fragaria × ananassa)

Cockerton¹,

Stavridou³

et al. 2020

BMC Plant Biol

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Background: Phosphate is an essential plant macronutrient required to achieve maximum crop yield. Roots are able to uptake soil phosphate from the immediate root area, thus creating a nutrient depletion zone. Many plants are able to exploit phosphate from beyond this root nutrient depletion zone through symbiotic association with Arbuscular Mycorrhizal Fungi (AMF). Here we characterise the relationship between root architecture, AMF association and low phosphate tolerance in strawberries. The contrasting root architecture in the parental strawberry cultivars 'Redgauntlet' and 'Hapil' was studied through a mapping population of 168 progeny. Low phosphate tolerance and AMF association was quantified for each genotype to allow assessment of the phenotypic and genotypic relationships between traits. Results:A "phosphate scavenging" root phenotype where individuals exhibit a high proportion of surface lateral roots was associated with a reduction in root system size across genotypes. A genetic correlation between "root system size" traits was observed with a network of pleiotropic QTL found to represent five "root system size" traits. By contrast, average root diameter and the distribution of roots appeared to be under two discrete methods of genetic control. A total of 18 QTL were associated with plant traits, 4 of which were associated with solidity that explained 46% of the observed variation. Investigations into the relationship between AMF association and root architecture found that a higher root density was associated with greater AMF colonisation across genotypes. However, no phenotypic correlation or genotypic association was found between low phosphate tolerance and the propensity for AMF association, nor root architectural traits when plants are grown under optimal nutrient conditions.(Continued on next page)

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Identifying Verticillium dahliae Resistance in Strawberry Through Disease Screening of Multiple Populations and Image Based Phenotyping

Cited by 26 publications

References 69 publications

Accuracy of genomic selection and long‐term genetic gain for resistance to Verticillium wilt in strawberry

Accuracy of genomic selection and long‐term genetic gain for resistance to Verticillium wilt in strawberry

Hop Polyphenols in Relation to Verticillium Wilt Resistance and Their Antifungal Activity

Genetic and phenotypic associations between root architecture, arbuscular mycorrhizal fungi colonisation and low phosphate tolerance in strawberry (Fragaria × ananassa)

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