Identity of a powdery mildew fungus occurring on Paeonia and its relationship with Erysiphe hypophylla on oak

Takamatsu, Susumu; Limkaisang, Saranya; Kom-un, Sawwanee; Bolay, A.; To-anun, Chaiwat

doi:10.1007/s10267-006-0317-5

Cited by 26 publications

(17 citation statements)

References 10 publications

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“…ITS A was identical to most (seven of 10; the three others differing by only one base) of the ITS sequences of E. alphitoides deposited in GenBank, e.g. collected on Quercus petraea in Switzerland (T akamatsu et al. 2006) and on Q. robur in Germany (H euser and Z immer 2002).…”

Section: Resultsmentioning

confidence: 74%

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New insights into the identity and origin of the causal agent of oak powdery mildew in Europe

2008

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Powdery mildew is the most common disease on oaks in Europe where it was first recorded at the beginning of the 20th century. Yet, little is known about the origin of the causal agent. In this study, we analysed the variability of the ribosomal DNA (rDNA) of the pathogen. The internal transcribed spacer (ITS) region, the 5.8S rRNA coding gene and the intergenic spacer (IGS) of the rDNA of 33 European (mostly French) samples of oak powdery mildew were amplified using polymerase chain reaction (PCR) and PCR products were subsequently sequenced. Four different haplotypes were obtained for ITS among the various samples (ITS A , ITS B , ITS C and ITS D ). Each ITS sequence corresponded to a different IGS sequence. The comparison of ITS sequences obtained with sequences accessible in the GenBank database revealed very high homologies with different taxa. Of these, three taxa had already been described on oaks in Europe, i.e. Erysiphe alphitoides (100% homology with ITS A ), Erysiphe hypophylla (99.4% homology with ITS C ) and Phyllactinia guttata (97.64% homology with ITS D ). Our data also confirmed the 100% homology between ITS A and the sequence described for Oïdium mangiferae, the agent of mango powdery mildew. The fourth haplotype, i.e. ITS B , represented by nearly 25% samples, showed 100% homology with the recently described Erysiphe quercicola from Quercus spp. in Asia, and several tropical and sub-tropical powdery mildew species, including Oïdium heveae, a major pathogen of rubber trees worldwide. Our results suggest that oak powdery mildew might originate from host shifts of tropical Erysiphe species introduced to Europe through infected exotic host plants.

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Section: Resultsmentioning

confidence: 74%

“…2006) and on Q. robur in Germany (H euser and Z immer 2002). The same sequence was also described for a number of other species: E. hypophylla isolated from Q. robur and Paeonia lutea in Switzerland (T akamatsu et al. 2006), Oïdium mangiferae from Mangifera indica in Australia (L imkaisang et al.…”

Section: Resultsmentioning

confidence: 77%

New insights into the identity and origin of the causal agent of oak powdery mildew in Europe

2008

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“…The phylogeny of Erysiphales was extensively revisited (Hirata and Takamatsu 1996;Takamatsu et al 1998Takamatsu et al , 1999Takamatsu et al , 2006Takamatsu et al , 2007Saenz and Taylor 1999;Braun and Takamatsu 2000;Cunnington et al 2003;Takamatsu 2004;Limkaisang et al 2005Limkaisang et al , 2006Braun et al 2006). In particular, molecular studies of numerous powdery mildew species in the former Erysiphe, Microsphaera, and Uncinula genera showed that they formed a separate, monophyletic clade, characterized by common morphological characters.…”

Section: Interspecific Diversitymentioning

confidence: 99%

Interspecific and intraspecific diversity in oak powdery mildews in Europe: coevolution history and adaptation to their hosts

et al. 2011

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Quercus has been reported as the genus with the largest number of attacking powdery mildews. In Europe, oak powdery mildew was rarely reported before 1907, when severe outbreaks were observed. These epidemics were attributed to the newly described species Erysiphe alphitoides, presumed to be of exotic origin. After the burst of interest following the emergence of the disease, research on this topic remained very limited. Interest in research was recently reactivated in response to the availability of molecular tools. This review summarizes current knowledge on the diversity of oak powdery mildews in Europe and their possible evolutionary relationships with European oaks. The most striking results are the evidence of cryptic diversity (detection in France of a lineage closely related to Erysiphe quercicola, previously thought to only have an Asian distribution), large host range (similarity of E. alphitoides and E. quercicola with powdery mildews of tropical plants) but also local adaptation to Quercus robur. These recent findings highlight the complexity of the history of oak powdery mildew in Europe and point to the question of host specialization and host jumps in the evolution of powdery mildew fungi.

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“…& U. Braun and not yet recorded in Europe (Limkaisang et al, 2005;Takamatsu et al, 2006). The occurrence of several species, or at least genetically differentiated groups, could have important epidemiological implications, with the severity of the disease possibly linked to the behaviour of each group.…”

Section: Introductionmentioning

confidence: 99%

Spatial distribution of lineages of oak powdery mildew fungi in France, using quick molecular detection methods

Mougou‐Hamdane

Giresse²,

Dutech

et al. 2010

Ann. For. Sci.

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Abstract• Powdery mildew is a major fungal disease of oaks in Europe. Recent studies using internal transcribed spacer (ITS) sequences suggested the presence of four different lineages (putative species). The objective of the study was to investigate the spatial distribution of these lineages/species and, in particular, to test the hypothesis of a spatial differentiation, at various scales: regional (France), altitudinal (a Pyrenean transect) and local (within a forest plot).• Detection methods for the four ITS types were developed: (1) single strand conformation polymorphism analysis (SSCP); (2) PCR amplifications, for which specific primers were designed. SSCP proved to be efficient for the detection of Erysiphe alphitoides and E. quercicola types. In contrast, the rarer ITS types corresponding to E. hypophylla and Phyllactinia guttata (sensu lato) were only detected by specific amplification.• The study confirmed the strong predominance of the ITS sequence associated with E. alphitoides at all spatial scales (with a frequency higher than 80%). Isolates presumably belonging to E. quercicola (i.e. with same ITS type), a recently described species not yet recorded in Europe, were also found in all French regions at a significant frequency (15% at national level).• No pattern of spatial differentiation between the putative species could be demonstrated: E. alphitoides was often found in association with different ITS types in the same region, the same tree, and even in the same lesion. Mots-clés :Erysiphe alphitoides / Erysiphe quercicola / espèces sryptiques / Erysiphales / ITS / invasion biologique Résumé -Distribution spatiale de lignées d'oïdium des chênes en France analysée à l'aide d'outils rapides de caractérisation moléculaire.• L'oïdium est l'une des maladies fongiques les plus importantes sur chênes en Europe. Des études récentes basées sur l'internal transcribed spacer (ITS) ont suggéré l'implication de quatre lignées (espèces putatives) différentes. L'objectif de l'étude était de caractériser la distribution spatiale de ces lignées/espèces et en particulier de tester l'hypothèse d'une différenciation spatiale à plusieurs échelles : régionale (France), altitudinale (transect pyrénéen) et locale (au sein d'une parcelle forestière).• Des méthodes de détection des quatre types d'ITS ont été développées : (1) analyse SCCP ; (2) amplification par PCR de l'ITS par des amorces spécifiques. La SSCP a permis de détecter facilement les ITS correspondant à E. alphitoides et E. quercicola. Par contre, les ITS plus rares correspondant à E. hypophylla et Phyllactinia guttata (sensu lato) n'ont été détectés que par amplification spécifique.• L'étude a confirmé la forte prédominance (avec une fréquence supérieure à 80 %) de la séquence d'ITS associée à E. alphitoides. Les isolats présentant la même séquence d'ITS qu'E. quercicola, espèce récemment décrite et encore non mentionnée en Europe, ont également été retrouvés dans toutes les régions françaises à une fréquence importante (15 % en moyenne pour l'échantillonnage...

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Identity of a powdery mildew fungus occurring on Paeonia and its relationship with Erysiphe hypophylla on oak

Cited by 26 publications

References 10 publications

New insights into the identity and origin of the causal agent of oak powdery mildew in Europe

New insights into the identity and origin of the causal agent of oak powdery mildew in Europe

Interspecific and intraspecific diversity in oak powdery mildews in Europe: coevolution history and adaptation to their hosts

Spatial distribution of lineages of oak powdery mildew fungi in France, using quick molecular detection methods

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