Identity of Activation Molecule 3 on Superantigen-Stimulated Bovine Cells Is CD26

Lee, Sang-Un; Ferens, Witold A.; Davis, William C.; Hamilton, Mary Jo; Park, Yong‐Ho; Fox, Lawrence K.; Naessens, Jan; Bohach, Gregory A.

doi:10.1128/iai.69.11.7190-7193.2001

Cited by 11 publications

(9 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Bands were visualized by silver staining (SilverQuest™ Staining Kit, Invitrogen, CA). Comparison of the band patterns revealed which band was specific for the mAb defined molecule as previously shown (Lee et al, 2001). Where possible, the specific band was further confirmed by WB with the mAb used for the IP following reducing and non-reducing SDS-PAGE conditions.…”

Section: Methodssupporting

confidence: 65%

“…A mAb designated as ACT3 clustered with mAbs submitted to the second ruminant workshop that formed workshop cluster WC10 (Naessens and Hopkins, 1996). Further studies showed the mAb ACT3 recognized CD26 (Lee et al, 2001). As mentioned below, this mAb was used in the validation of the specificity of a cluster of mAbs (ACT40) developed at a later date.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Characterization and expression of monoclonal antibody-defined molecules on resting and activated bovine αβ, γδ T and NK cells

Park

Seo

Godwin

et al. 2015

Veterinary Immunology and Immunopathology

Self Cite

View full text Add to dashboard Cite

Monoclonal antibodies (mAbs) specific for leukocyte differentiation molecules (LDMs) were developed during the past few decades to expand reagents for research in ruminants, pigs, and horses. The specificity of some of the mAb-defined molecules was determined through participation in international workshops. Other molecules identified with mAbs during this time, and more recently with mAbs developed after the workshops, have remained partially characterized. Efforts are now underway to characterize the specificity of these mAbs. As reported here, flow cytometry (FC) was used to screen two sets of hybridomas to determine how many of the hybridomas produce mAbs that detect molecules with up-regulated expression on activated lymphocytes or NK cells. Thirty four hybridomas were identified. Comparison of the patterns of reactivity of the mAbs showed some of the mAbs formed clusters that recognize 5 different molecules. FC showed one cluster recognized CD25. Use of mass spectrometry showed 4 clusters recognized orthologues of CD26, CD50, gp96 and signaling lymphocytic activation molecule family member 9 (SLAMF9). Verification and documentation that CD26, CD50, and SLAMF9 were only up-regulated on activated cells was obtained with PBMC from calves vaccinated with a Mycobacterium avium paratuberculosis mutant, Map-relA. CD26 and CD50 were up-regulated on NK cells, CD4 and CD8 T cells and γδ T cells. SLAMF9 was only up-regulated on CD4, CD8, and γδ T cells. gp96 was detected on granulocytes, monocytes and activated NK cells. Detection was attributable to the binding of gp96 to its receptor CD91.

show abstract

Section: Methodssupporting

confidence: 65%

Section: Resultsmentioning

confidence: 99%

Characterization and expression of monoclonal antibody-defined molecules on resting and activated bovine αβ, γδ T and NK cells

Park

Seo

Godwin

et al. 2015

Veterinary Immunology and Immunopathology

Self Cite

View full text Add to dashboard Cite

show abstract

“…To date, these ACT molecules remain incompletely characterized, and data presented here will be beneficial as their identities become characterized (e.g., CD26 was formerly ACT3) (26). Regardless, CD4 ϩ , CD8 ϩ , and ␥␦ TCR ϩ cells from M. bovisinfected cattle exhibit an activated phenotype when restimulated with either an rESAT-6-CFP10 fusion protein or M. bovis PPD.…”

Section: Cd45romentioning

confidence: 99%

“…Additionally, there was a notable increase in the number of cells expressing CD26. The cellular upregulation of CD26, an ectoenzyme, has been associated with an activated phenotype in cattle (26). CD4 ϩ , CD8 ϩ , and ␥␦ TCR ϩ cells from M. bovis-infected cattle, but not from noninfected animals, upregulated their expression of CD26 at 6 days of culture with mycobacterial antigens (Fig.…”

Section: Pbmcs From M Bovis-infected Cattle Exhibit Robust Proliferamentioning

confidence: 99%

Analysis of Immune Responses Directed toward a Recombinant Early Secretory Antigenic Target Six-Kilodalton Protein-Culture Filtrate Protein 10 Fusion Protein inMycobacterium bovis-Infected Cattle

et al. 2005

Self Cite

View full text Add to dashboard Cite

Cell-mediated immune responses are critical for protective immunity to mycobacterial infections. Recent progress in defining mycobacterial antigens has determined that region of difference 1 (RD1) gene products induce strong T-cell responses, particularly the early secretory antigenic target 6-kDa (ESAT-6) protein and culture filtrate protein 10 (CFP10). However, comprehensive analysis of the immune response towards these antigens is incompletely characterized. To evaluate recall responses to ESAT-6 and CFP10, peripheral blood mononuclear cells from M. bovis-infected cattle were stimulated in vitro with a recombinant ESAT-6 (rESAT-6)-CFP10 fusion protein and compared to responses induced by M. bovis-derived purified protein derivative. Following antigenic stimulation, activation marker expression was evaluated. Significant proliferative responses (P < 0.05) were evident in CD4 ؉ , CD8 ؉ , immunoglobulin M-positive, and CD172a ؉ cell fractions after 6 days of culture. Expression of CD25 and CD26 was increased (P < 0.05) on CD4 ؉ , CD8 ؉ , and ␥␦ T-cell-receptor-positive cells. CD4؉ and CD8 ؉ cells also exhibited significant changes (P < 0.05) in expression of CD45 isoforms. Using a flow cytometry-based proliferation assay, it was determined that CD45R expression is downregulated (P < 0.05) and that CD45RO expression is upregulated (P < 0.05) on proliferating (i.e., activated) CD4 ؉ cells. Collectively, data indicate that recall immune responses directed toward the rESAT-6-CFP10 fusion protein or purified protein derivative are comparable and that recall to mycobacterial antigens correlates with a CD45RO ؉ phenotype.

show abstract

“…4, below). MAbs specific for CD25 and CD26 were used to monitor the activation status of ␣␤ and ␥␦ T cells and other cells in the cultures (23,40). An additional MAb was used to monitor the upregulation of a molecule, ACT2, uniquely expressed on activated ␥␦ T cells ( Table 2; Fig.…”

Section: Wc1mentioning

confidence: 99%

Analysis of the Immune Response toMycobacterium aviumsubsp.paratuberculosisin Experimentally Infected Calves

Koo

Park

Hamilton³

et al. 2004

Infect Immun

Self Cite

View full text Add to dashboard Cite

Johne's disease of cattle is widespread and causes significant economic loss to producers. Control has been hindered by limited understanding of the immune response to the causative agent, Mycobacterium avium subsp. paratuberculosis, and lack of an effective vaccine and sensitive specific diagnostic assays. The present study was conducted to gain insight into factors affecting the immune response to M. avium subsp.

show abstract

Identity of Activation Molecule 3 on Superantigen-Stimulated Bovine Cells Is CD26

Cited by 11 publications

References 36 publications

Characterization and expression of monoclonal antibody-defined molecules on resting and activated bovine αβ, γδ T and NK cells

Characterization and expression of monoclonal antibody-defined molecules on resting and activated bovine αβ, γδ T and NK cells

Analysis of Immune Responses Directed toward a Recombinant Early Secretory Antigenic Target Six-Kilodalton Protein-Culture Filtrate Protein 10 Fusion Protein inMycobacterium bovis-Infected Cattle

Analysis of the Immune Response toMycobacterium aviumsubsp.paratuberculosisin Experimentally Infected Calves

Contact Info

Product

Resources

About