Identity of the xerophilic species &lt;i&gt;Aspergillus penicillioides&lt;/i&gt;: Integrated analysis of the genotypic and phenotypic characters

Tamura, Miki; Kawasaki, Hiroko; Sugiyama, Junta

doi:10.2323/jgam.45.29

Cited by 17 publications

(11 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…known to produce sterigmatocystin, as A. versicolor and Emericella nidulans. However, very few studies have addressed methoxysterigmatocystin, and those studies were mostly concerned with contamination by A. versicolor (Klich, 2009;Nielsen et al, 1999 (Frisvad, 2015;Itabashi et al, 2006;Peterson, 2008;Tamura et al, 1999). For A. vitricola, in fact, no records were detected.…”

Section: Discussionmentioning

confidence: 99%

Fungal secondary metabolite analysis applied to Cultural Heritage: the case of a contaminated library in Venice

Micheluz

Sulyok²,

Manente

et al. 2016

WMJ

View full text Add to dashboard Cite

The secondary metabolite production of several fungal strains of Aspergillus creber, Aspergillus jensenii, Aspergillus penicillioides, Aspergillus protuberus, Aspergillus vitricola, Cladosporium cladosporioides, Eurotium chevalieri, Eurotium halophilicum, Penicillium brevicompactum and Penicillium chrysogenum were characterised by liquid chromatography tamdem mass spectometry. All fungi were isolated from both air and book covers as well as from settled dust from a contaminated library in Venice (Italy). For A. creber and A. jensenii, we identified sterigmatocystin, methoxysterigmatocystin, versicolorin A and related precursors/side metabolites from the biosynthetic pathways. Deoxybrevianamid E, neoechinulin A, pseurotin A and D, and rugulusovin were principally detected from the strains of E. halophilicum, an emerging fungal species implicated in book contaminations in specific indoor niches. The analysis of settled dust showed a wide range of toxic or bioactive fungal metabolites. Forty-five different metabolites were identified in different concentrations; in particular, high amounts of asperglaucide, alamethicin, andrastin A, terrecyclic acid and neoechinulin A were detected. Also one bacterial metabolite, chloramphenicole was detected. This study increases the knowledge about metabolite production of several fungal species, as well as on the indoor presence of fungi that are not detected by aerobiological sampling. These results emphasise how routine dusting operations are necessary and essential in order to prevent further microbiological developments in library environments.

show abstract

Section: Discussionmentioning

confidence: 99%

Fungal secondary metabolite analysis applied to Cultural Heritage: the case of a contaminated library in Venice

Micheluz

Sulyok²,

Manente

et al. 2016

WMJ

View full text Add to dashboard Cite

show abstract

“…Clavati. Based on the results of this study and former studies (Tamura et al, 1996(Tamura et al, , 1999, we concluded that species of Aspergillus showing DNA relatedness at 70% or greater are conspecific, as was shown for the yeasts (Kurtzman, 1987).…”

Section: Resultsmentioning

confidence: 91%

“…In recent years, molecular genetic approaches have been extended to filamentous fungi such as Aspergillus and related teleomorphs (Kurtzman et al, 1986;Peterson, 1992;Sugiyama et al, 1991;Tamura et al, 1999). Peterson (1999) studied the D1 and D2 regions of the large subunit ribosomal RNA genes (lsu-rDNA) from 215 named taxa in Aspergillus and tried to establish a species concept of these taxa.…”

mentioning

confidence: 99%

Genetic relatedness among species in Aspergillus section Clavati as measured by electrophoretic comparison of enzymes, DNA base cmposition, and DNA-DNA hybridization.

Tamura

Hamamoto

Cañete‐Gibas

et al. 1999

J. Gen. Appl. Microbiol.

Self Cite

View full text Add to dashboard Cite

“…Alternatively, as described above, DNA-DNA relatedness values >80.0% indicate the plausibility of a single genospecies. However, previous studies on yeasts and moulds reported 70-79% DNA-DNA relatedness between multiple strains of the same species [18]. In view of the 76.8% DNA-DNA relatedness between MAFF 238035 F. proliferatum and CBS 221.76 F. fujikuroi in this study, we must carefully discuss discreetly whether F. proliferatum and F. fujikuroi should be considered the same biological species or distinguished as different species.…”

mentioning

confidence: 81%

“…In previous studies on genetic distances between closelyrelated fungal species and identification of fungal isolates [18], whole-genome sequence relatedness was measured using a DNA-DNA hybridization technique (DNA-DNA relatedness). In DNA-DNA hybridization experiments, DNA probes are generally labeled with radioisotopes or biotin.…”

mentioning

confidence: 99%

Sensitive Detection of Whole-Genome Differentiation among Closely-Related Species of the Genus <i>Fusarium</i> Using DNA-DNA Hybridization and a Microplate Technique

Watanabe

Goto²,

Sugita-Konishi

et al. 2012

J. Vet. Med. Sci.

View full text Add to dashboard Cite

ABSTRACT. We developed a new system for detection of whole-genome differentiation using DNA-DNA hybridization, and tested its sensitivity with three closely-related Fusarium species. We compared DNA-DNA relatedness to nucleotide sequence homologies of five genetic regions between each of five strains of three Fusarium species. DNA-DNA relatedness by our system was 16.2-86.6%. Sequence homologies of 18S rDNA, rDNA cluster region from ITS1 to 28S rDNA, β-tub, EF-1α and lys2 were 100.0, 99.0-100.0, 96.7-100.0, 95.1-99.4, and 94.7-100.0%, respectively. Our system could clearly detect differentiation between closely-related fungal species which have very similar morphological-characteristics, and exhibit little diagnoses in nucleotide sequences. Our results suggest that this system is a good tool for identification and phylogenetic analysis of closely-related fungal species. A microbial species concept has been defined on the basis of whole-genome sequence relatedness, which corresponds well with biological relatedness (bacteria: [13]; yeast: [10]). In previous studies on genetic distances between closelyrelated fungal species and identification of fungal isolates [18], whole-genome sequence relatedness was measured using a DNA-DNA hybridization technique (DNA-DNA relatedness). In DNA-DNA hybridization experiments, DNA probes are generally labeled with radioisotopes or biotin. Recently, digoxigenin (DIG) has been used for the nonradioactive labeling of DNA probes. In a study performed to detect amplified virus DNA, sensitivity of the DIG-labeled probe was equivalent to that of a radioactive probe, and has a clearly higher sensitivity than that of a photobiotinylated probe [11]. Assays for DNA-DNA relatedness using the DIG system were performed with dot-blot hybridization on nitrocellulose membranes [15]. On the other hand, the 96-well microplate technique has been developed as a convenient method for binding DNAs in DNA-DNA hybridization. In this technique, a probe is hybridized with unlabeled DNA bound on the surface of microplate wells. This new technique has been used in systematics and for the identification of bacteria [4]. However, all previous studies using this technique were performed with photobiotinylated probes, which have low sensitivity of detection, as described above, and none of the studies were performed with fungal DNAs.Fusarium species are one of the most important fungi, well known as human, animal, and plant pathogens. They cause infectious diseases of the eye and opportunistic mycosis for humans and animals [8]. Furthermore, they produce many kinds of mycotoxins, T-2 toxin, nivalenol, fumonisin, and others. Mycotoxicoses are caused in the world by the contaminated food and feed [9]. Therefore, rapid and accurate identification of Fusarium species is essential in the fields of medicine and food hygienics. The detection of species-specific differentiation among Fusarium species using nucleotide sequence homologies of certain genes is sometimes difficult because of the little diagnoses in sequence...

show abstract

Identity of the xerophilic species <i>Aspergillus penicillioides</i>: Integrated analysis of the genotypic and phenotypic characters

Cited by 17 publications

References 33 publications

Fungal secondary metabolite analysis applied to Cultural Heritage: the case of a contaminated library in Venice

Fungal secondary metabolite analysis applied to Cultural Heritage: the case of a contaminated library in Venice

Genetic relatedness among species in Aspergillus section Clavati as measured by electrophoretic comparison of enzymes, DNA base cmposition, and DNA-DNA hybridization.

Sensitive Detection of Whole-Genome Differentiation among Closely-Related Species of the Genus <i>Fusarium</i> Using DNA-DNA Hybridization and a Microplate Technique

Contact Info

Product

Resources

About