Idiopathic Colitis in Rhesus Macaques Is Associated With Dysbiosis, Abundant Enterochromaffin Cells and Altered T-Cell Cytokine Expression

Laing, Steven T.; Merriam, David; Shock, Barbara C.; Mills, Sarah; Spinner, Abbie; Reader, Rachel; Hartigan-O’Connor, Dennis J.

doi:10.1177/0300985818780449

Cited by 33 publications

(65 citation statements)

References 49 publications

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“…Alternatively, these Helicobacter species could be more susceptible to antibiotics than Campylobacter resulting in its depletion. Indeed, a recent study using rhesus macaques reported a large decrease in Helicobacter following vancomycin treatment despite the observation that Helicobacter should not be directly susceptible to vancomycin [87, 88].…”

Section: Discussionmentioning

confidence: 99%

Maturation of the infant rhesus macaque gut microbiome and its role in the development of diarrheal disease

et al. 2019

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Background Diarrhea is the second leading cause of death in children under 5 years of age. Enhanced understanding of causal pathways, pathogenesis, and sequelae of diarrhea is urgently needed. Although the gut microbiota is believed to play a role in susceptibility to diarrheal diseases, our understanding of this association remains incomplete. Infant rhesus macaques ( Macaca mulatta ) are susceptible to diarrhea making them an ideal model to address this question. Results The maturation of the infant rhesus macaque gut microbiome throughout the first 8 months of life occurs in a similar pattern as that described for human infants. Moreover, the microbiome of the captive reared infant rhesus macaque more closely resembles that of human infants in the developing world than in the western world. Importantly, prior to disease onset, the gut microbiome of infants that later develop diarrhea is enriched in pathways of immunomodulatory metabolite synthesis, while those of infants that remain asymptomatic are enriched in pathways for short-chain fatty acid production. We identify Prevotella strains that are more abundant at 1 month in infants that later develop diarrhea. At 8 months, the microbiomes of animals that experience diarrhea show increased abundance of Campylobacter and a reduction in Helicobacter macacae . Conclusion The composition of the microbial community could provide a phenotypic marker of an infant’s susceptibility to diarrheal disease. Given the significant physiological and immunological similarities between human and nonhuman primates, these findings provide potential markers of susceptibility to diarrhea that could be modulated to improve infant health, especially in the developing world. Electronic supplementary material The online version of this article (10.1186/s13059-019-1789-x) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Maturation of the infant rhesus macaque gut microbiome and its role in the development of diarrheal disease

et al. 2019

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“…P . hominis has been associated with chronic diarrhoea and/or inflammatory bowel disease in animals [56], nonhuman primates [57] and humans [58], and is found in 14% of pregnant women in Papua New Guinea. In our study affected VL cases had higher abundance of P .…”

Section: Discussionmentioning

confidence: 99%

Meta-taxonomic analysis of prokaryotic and eukaryotic gut flora in stool samples from visceral leishmaniasis cases and endemic controls in Bihar State India

et al. 2019

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Visceral leishmaniasis (VL) caused by Leishmania donovani remains of public health concern in rural India. Those at risk of VL are also at risk of other neglected tropical diseases (NTDs) including soil transmitted helminths. Intestinal helminths are potent regulators of host immune responses sometimes mediated through cross-talk with gut microbiota. We evaluate a meta-taxonomic approach to determine the composition of prokaryotic and eukaryotic gut microflora using amplicon-based sequencing of 16S ribosomal RNA (16S rRNA) and 18S rRNA gene regions. The most abundant bacterial taxa identified in faecal samples from Bihar State India were Prevotella (37.1%), Faecalibacterium (11.3%), Escherichia-Shigella (9.1%), Alloprevotella (4.5%), Bacteroides (4.1%), Ruminococcaceae UCG-002 (1.6%), and Bifidobacterium (1.5%). Eukaryotic taxa identified (excluding plant genera) included Blastocystis (57.9%; Order: Stramenopiles), Dientamoeba (12.1%; Family: Tritrichomonadea), Pentatrichomonas (10.1%; Family: Trichomonodea), Entamoeba (3.5%; Family: Entamoebida), Ascaridida (0.8%; Family: Chromodorea; concordant with Ascaris by microscopy), Rhabditida (0.8%; Family: Chromodorea; concordant with Strongyloides), and Cyclophyllidea (0.2%; Order: Eucestoda; concordant with Hymenolepis). Overall alpha (Shannon’s, Faith’s and Pielou’s indices) and beta (Bray-Curtis dissimilarity statistic; weighted UniFrac distances) diversity of taxa did not differ significantly by age, sex, geographic subdistrict, or VL case (N = 23) versus endemic control (EC; N = 23) status. However, taxon-specific associations occurred: (i) Ruminococcaceae UCG- 014 and Gastranaerophilales_uncultured bacterium were enriched in EC compared to VL cases; (ii) Pentatrichomonas was more abundant in VL cases than in EC, whereas the reverse occurred for Entamoeba. Across the cohort, high Escherichia-Shigella was associated with reduced bacterial diversity, while high Blastocystis was associated with high bacterial diversity and low Escherichia-Shigella. Individuals with high Blastocystis had low Bacteroidaceae and high Clostridiales vadin BB60 whereas the reverse held true for low Blastocystis. This scoping study provides useful baseline data upon which to develop a broader analysis of pathogenic enteric microflora and their influence on gut microbial health and NTDs generally.

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“…40 Increased abundance of Helicobacter brantae was shown in a batch of commercially hatched broiler parent chickens (over 80% of ileal microbiota in individual chickens). 40 Increased abundance of Helicobacter brantae was shown in a batch of commercially hatched broiler parent chickens (over 80% of ileal microbiota in individual chickens).…”

Section: Pre S En Ce Of Non -H Elicobac Ter Pylori Helicobac Ter S mentioning

confidence: 99%

“…On the other hand, the analysis of 35 rhesus macaques euthanized for ICD demonstrated that ICD was associated with dysbiosis involving Campylobacter and Trichomonas, and a loss of Helicobacter macacae. 40 Increased abundance of Helicobacter brantae was shown in a batch of commercially hatched broiler parent chickens (over 80% of ileal microbiota in individual chickens). 41 Eimeria parasite coccidiosis is an economically important disease of poultry and livestock worldwide.…”

Section: Pre S En Ce Of Non -H Elicobac Ter Pylori Helicobac Ter S mentioning

confidence: 99%

Review: Other Helicobacter species

Ménard

Smet

2019

Helicobacter

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This article is a review of the most important, accessible, and relevant literature published between April 2018 and April 2019 in the field of Helicobacter species other than Helicobacter pylori. The initial part of the review covers new insights regarding the presence of gastric and enterohepatic non‐H. pylori Helicobacter species (NHPH) in humans and animals, while the subsequent section focuses on the progress in our understanding of the pathogenicity and evolution of these species. Over the last year, relatively few cases of gastric NHPH infections in humans were published, with most NHPH infections being attributed to enterohepatic Helicobacters. A novel species, designated “Helicobacter caesarodunensis,” was isolated from the blood of a febrile patient and numerous cases of human Helicobacter cinaedi infections underlined this species as a true emerging pathogen. With regard to NHPH in animals, canine/feline gastric NHPH cause little or no harm in their natural host; however they can become opportunistic when translocated to the hepatobiliary tract. The role of enterohepatic Helicobacter species in colorectal tumors in pets has also been highlighted. Several studies in rodent models have further elucidated the mechanisms underlying the development of NHPH‐related disease, and the extra‐gastric effects of a Helicobacter suis infection on brain homeostasis was also studied. Comparative genomics facilitated a breakthrough in the evolutionary history of Helicobacter in general and NHPH in particular. Investigation of the genome of Helicobacter apodemus revealed particular traits with regard to its virulence factors. A range of compounds including mulberries, dietary fiber, ginseng, and avian eggs which target the gut microbiota have also been shown to affect Helicobacter growth, with a potential therapeutic utilization and increase in survival.

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Idiopathic Colitis in Rhesus Macaques Is Associated With Dysbiosis, Abundant Enterochromaffin Cells and Altered T-Cell Cytokine Expression

Cited by 33 publications

References 49 publications

Maturation of the infant rhesus macaque gut microbiome and its role in the development of diarrheal disease

Maturation of the infant rhesus macaque gut microbiome and its role in the development of diarrheal disease

Meta-taxonomic analysis of prokaryotic and eukaryotic gut flora in stool samples from visceral leishmaniasis cases and endemic controls in Bihar State India

Review: Other Helicobacter species

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