Idiotypic determinants on T-cell subpopulations.

Abstract

Killer T cells with specificity for major histocompatibility antigens have been shown in mice and rats to display idiotypic receptors allowing the lysis of such cells at the effector phase by anti-idiotypic antibodies and complement. A comparison was made between idiotypes displayed by Lyt-1-2+3+ and Lyt-1+2-3- T blasts, generated in the same mixed leucocyte culture (MLC), across an entire H-2 locus barrier. This was done by absorption of anti-idiotypic antibodies with respective T blasts, followed by estimation of the ability of the absorbed antiserum to inhibit MLC or killer T-cell function. Further, the capacity of Lyt-purified, MLC-generated T blasts to provoke specific unresponsiveness via anti-idiotypic immunity in syngeneic recipients was analyzed. Taken together, the results demonstrate that Lyt-1+2-3- T blasts responsible for the major part of MLC proliferation have distincly different idiotypes from those on the Lyt 1-2+3+ killer T cells. That the idiotypes on the killer T-cell presursors can serve as triggering sites for induction of effector T-cell function was then suggested by experiments with Lyt-1-2+3+-purified, normal T cells as precursor cells in vitro. The fact that autoanti-idiotypic antibodies may circumvent the need for helper Lyt-1+2-3- T cells in the generation of allospecific killer T cells indicates that the former cells may normally function partly via such anti-idiotypic reactions.

Studies on Hapten Specific T Cell Immunity and Suppression

“…The essential distinction being that antigen plus self structures are considered necessary and required to activate T cells. This distinction is important and confirms and extends the recent studies of Binz et al (1979) in which anti-idiotype (antireceptor) binding to receptor apparently imparted sufficient cell signals to activate the relevant cell subpopulation of Lyt T or Lyt 23* cells. However, the possibility exists that anti-idiotype acts on T cells after presentation by macrophages and in conjunction with their la molecules.…”

Hapten-specific T cell responses to 4-hydroxy-3-nitrophenyl acetyl. VI. Evidence for different T cell receptors in cells that mediate H-21-restricted and H-2D-restricted cutaneous sensitivity responses.

“…These two cell populations may bear distinct surface receptors, leading to their functional divergence: the first being DTHlike, I-restricted, and unable to give NIP-cross-reactive NP-induced responses in non-Igh-I b strains of mice; the second being CTL-iike, K/D-restricted, and having a different pattern or NIP-cross-reactivity, consistent with the observation that NPspecific CTL lack the NP b idiotype (22)(23)(24). Binz et al (25) have reported the observation that different spectra of idiotypes exist on two subgroups of T cells generated in the same mixed leukocyte culture, namely, allo-Ia-binding Ly 1 + 2blasts and H-2K/D-binding Ly-2+ killer cells. The evidence in the present study suggests that such a dichotomy in idiotypic receptors between T cell subpopulations may be a generalized finding.…”

T cell receptors with allo-major histocompatibility complex specificity from rat and mouse. Similarity of size, plasmin susceptibility, and localization of antigen-binding region.

“…Some new findings were obtained. Previous studies using anti-idiotypic reagents had shown that similarly generated MLC T blasts of 1+,2-,3 -and 1-,2+,3 + phenotype carried distinctly different idiotypes, paralleling their distinctly different antigen-binding specificities (35,52). With the present antiserum, Lyt-l+,2-,3 -and 1-,2+,3 + blasts were both stained although the 2÷,3 + cells fluoresced more intensely.…”

“…Anti-Lyt 1.2, 2.2, and 3.2 were a generous gift of Dr. Fung W. Shen, Memorial Sloan-Kettering Cancer Center, New York; others were produced in Uppsala, Sweden, as described (35) or purchased from New England Nuclear. Anti-Lyt-1.2 antiserum was used at a final concentration of 1:30, anti-Lyt-2.2 at 1:40 and anti-Lyt-3.2 at 1:50.…”