IFCC Primary Reference Procedures for the Measurement of Catalytic Activity Concentrations of Enzymes at 37°C. Part 3. Reference Procedure for the Measurement of Catalytic Concentration of Lactate Dehydrogenase

Abstract

This paper is the third in a series dealing with reference procedures for the measurement of catalytic activity concentrations of enzymes at 37 degrees C and the certification of reference preparations. Other parts deal with: Part 1. The Concept of Reference Procedures for the Measurement of Catalytic Activity Concentrations of Enzymes; Part 2. Reference Procedure for the Measurement of Catalytic Concentration of Creatine Kinase; Part 4. Reference Procedure for the Measurement of Catalytic Concentration of Alanine Aminotransferase; Part 5. Reference Procedure for the Measurement of Catalytic Concentration of Aspartate Aminotransferase; Part 6. Reference Procedure for the Measurement of Catalytic Concentration of gamma-Glutamyltransferase; Part 7. Certification of Four Reference Materials tamyltransferase, Lactate Dehydrogenase, Alanine Aminotransferase and Creatine Kinase at 37 degrees C. A document describing the determination of preliminary upper reference limits is also in preparation. The procedure described here is deduced from the previously described 30 degrees C IFCC reference method (1). Differences are tabulated and commented on in Appendix 1.

Reliability of IFCC Method for Lactate Dehydrogenase Measurement in Lithium-Heparin Plasma Samples

“…[When comparing our data with the results published by The interferences from carryover effects have been reported before (2,6 ). To test cuvette carryover effects, we measured LD activity in patient samples in plastic lithium-heparin-gel tubes.…”

Glycemic control, micronutrients and some metabolic enzyme activity in type 2 diabetes

“…21 Lactate dehydrogenase catalyses the interconversion of lactic and pyruic acids, a hydrogen transfer enzyme that utilizes the coenzyme NAD+.…”

Progress and impact of enzyme measurement standardization

“…In the last 14 years, the IFCC has recommended RMPs for seven enzymes [12][13][14][15][16][17][18], which are now listed in the database of the Joint Committee on Traceability in Laboratory Medicine (JCTLM) [19]. Few enzyme reference materials (ERM) are also listed (for aspartate aminotransferase [AST], γ-glutamyltranspeptidase [γGT], and α-amylase [AMY]), whereas reproduction and characterization of new ERMs for alanine aminotransferase (ALT), creatine kinase (CK) and lactate dehydrogenase (LDH) are ongoing [20].…”