IFCC Primary Reference Procedures for the Measurement of Catalytic Activity Concentrations of Enzymes at 37°C. Part 2. Reference Procedure for the Measurement of Catalytic Concentration of Creatine Kinase

Schumann, G.; Bonora, R; Ceriotti, Ferruccio; Clerc-Renaud, Pascale; Ferrero, C. A.; Férard, G; Franck, Paul; Gella, F. Javier; Hoelzel, Wieland; Jørgensen, Poul J.; Kanno, Takashi; Kessne, Art; Klauker, Rainer; Kristiansen, N.; Lessinger, Jean‐Marc; Linsinger, Thomas P. J.; Misaki, Hideo; Panteghini, Mauro; Pauwels, J.; Schimmel, Heinz; Vialle, A.; Weidemann, Gerhard; Siekmann, Lothar

doi:10.1515/cclm.2002.110

Cited by 85 publications

(53 citation statements)

References 10 publications

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“…All tests were performed following kit instructions. Preciseness of methods and instrumentation agreed the SIBIOC criteria (Schumann et al 2002). Results are expressed in international units per litre for CK, AST and LDH, milligrams per decilitre for BUN and grams per decilitre for TP.…”

Section: Clinical Measuresmentioning

confidence: 93%

Effect of the duration of road transport on the physiology and meat quality of lambs

et al. 2014

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Abstract.To assess the effect of transport duration on animal welfare and meat quality of lambs, two trials were performed: Forty Ile-de-France · Merino lambs were used in Trial 1 and 40 Comisana lambs in Trial 2. In both trials, the lambs, aged between 14 and 16 weeks, were divided into two groups of 20 animals. One group was subjected to a 1-h period of transportation (T1) and the other to a 24-h period of transportation (T24), both in the same truck and arriving to the same slaughterhouse at the same time. The effect of transport on serum biochemistry variables (cortisol, aspartate transaminase, lactate dehydrogenase, blood urea nitrogen, creatine kinase, creatinine and total proteins), salivary cortisol, metabolites of cortisol in faeces, intra-ruminal temperature and meat quality (pH, conductivity, expressible juice, colour and shear force) was assessed. In both studies, the duration of transport did not affect serum and salivary cortisol concentration (P > 0.05). However, in Trial 2, lambs exposed to 24-h transport had a higher concentration of faecal cortisol metabolites than did those transported for 1 h (P < 0.05). Blood variables were not affected by transport in either trial (P > 0.05), with the exception of blood urea nitrogen which was higher in Trial 1 for the T24 group (P < 0.05) than it was to T1 group. Although no signs of dehydration were found, intra-ruminal loggers showed that animals did not drink during the transportation in the way they did before transport. In Trial 1, no effect of transport duration was found on meat-quality traits (P > 0.05). Nevertheless, in Trial 2, lambs exposed to 24-h transport had higher values of colour attribute of a* (red trend) and less tenderness or higher values of shear force (P < 0.05). The present study showed that although there is little effect on meat quality, signs of stress are detectable in lambs transported for 24 h. Therefore, in the case of lambs, the effect of long transportation periods must be considered more in terms of animal welfare than in terms of product quality.

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Section: Clinical Measuresmentioning

confidence: 93%

Effect of the duration of road transport on the physiology and meat quality of lambs

et al. 2014

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“…17 Laboratory studies included serum CK activity estimated with automated analyzers (Roche/ Hitachi Systems, Roche Diagnostics, Indianapolis, Ind) according to procedures recommended by the International Federation of Clinical Chemistry. 18 …”

Section: Clinical Perspective P 2039mentioning

confidence: 99%

Creatine Kinase Activity Is Associated With Blood Pressure

et al. 2006

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Background-We previously hypothesized that high activity of creatine kinase, the central regulatory enzyme of energy metabolism, facilitates the development of high blood pressure. Creatine kinase rapidly provides adenosine triphosphate to highly energy-demanding processes, including cardiovascular contraction, and antagonizes nitric oxide-mediated functions. Relatively high activity of the enzyme, particularly in resistance arteries, is thought to enhance pressor responses and increase blood pressure. Tissue creatine kinase activity is reported to be high in black people, a population subgroup with greater hypertension risk; the proposed effects of high creatine kinase activity, however, are not "race dependent." We therefore assessed whether creatine kinase is associated with blood pressure in a multiethnic population. Methods and Results-We analyzed a stratified random sample of the population of Amsterdam, the Netherlands, consisting of 1444 citizens (503 white European, 292 South Asian, 580 black, and 69 of other ethnicity) aged 34 to 60 years. We used linear regression analysis to investigate the association between blood pressure and normal serum creatine kinase after rest, as a substitute measure of tissue activity. Creatine kinase was independently associated with blood pressure, with an increase in systolic and diastolic pressure, respectively, of 8.0 (95% CI, 3.3 to 12.7) and 4.7 (95% CI, 1.9 to 7.5) mm Hg per log creatine kinase increase after adjustment for age, sex, body mass index, and ethnicity. Conclusions-Creatine kinase is associated with blood pressure. Further studies are needed to explore the nature of this association, including how variation in cardiovascular creatine kinase activity may affect pressor responses.

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“…In the last 14 years, the IFCC has recommended RMPs for seven enzymes [12][13][14][15][16][17][18], which are now listed in the database of the Joint Committee on Traceability in Laboratory Medicine (JCTLM) [19]. Few enzyme reference materials (ERM) are also listed (for aspartate aminotransferase [AST], γ-glutamyltranspeptidase [γGT], and α-amylase [AMY]), whereas reproduction and characterization of new ERMs for alanine aminotransferase (ALT), creatine kinase (CK) and lactate dehydrogenase (LDH) are ongoing [20].…”

Section: Introductionmentioning

confidence: 99%

Progress and impact of enzyme measurement standardization

Infusino

Frusciante

Braga

et al. 2017

Clinical Chemistry and Laboratory Medicine (CCLM)

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International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) has established reference measurement procedures (RMPs) for the most popular enzymes. Manufacturers should assign values to commercial calibrators traceable to these RMPs to achieve equivalent results in clinical samples, independent of reagent kits, instruments, and laboratory where the measurement is carried out. The situation is, however, far from acceptable. Some manufacturers continue to market assays giving results that are not traceable to internationally accepted RMPs. Meanwhile, end-users often do not abandon assays with demonstrated insufficient quality. Of the enzyme measurements, creatine kinase (CK) is satisfactorily standardized and a substantial improvement in performance of marketed γ-glutamyltranspeptidase (GGT) assays has been demonstrated. Conversely, aminotransferase measurements often exceed the desirable analytical performance because of the lack of pyridoxal-5-phosphate addition in the commercial reagents. Measurements of lactate dehydrogenase (LDH), alkaline phosphatase (ALP), and α-amylase (AMY) still show major disagreement, suggesting the need for improvement in implementing traceability to higher-order references. This is mainly the result of using assays with different analytical selectivities for these enzymes. The definition by laboratory professionals of the clinically acceptable measurement uncertainty for each enzyme together with the adoption by EQAS of commutable materials and use of an evaluation approach based on trueness represent the way forward for reaching standardization in clinical enzymology.

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IFCC Primary Reference Procedures for the Measurement of Catalytic Activity Concentrations of Enzymes at 37°C. Part 2. Reference Procedure for the Measurement of Catalytic Concentration of Creatine Kinase

Cited by 85 publications

References 10 publications

Effect of the duration of road transport on the physiology and meat quality of lambs

Effect of the duration of road transport on the physiology and meat quality of lambs

Creatine Kinase Activity Is Associated With Blood Pressure

Progress and impact of enzyme measurement standardization

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