IFN-γInduction by SCG, 1,3-β-D-Glucan fromSparassis crispa, in DBA/2 MiceIn Vitro

Harada, Toshie; Miura, Noriko N.; Adachi, Yoshiyuki; Nakajima, Mitsuhiro; Yadomae, Toshiro; Ohno, Naohito

doi:10.1089/10799900260475759

Cited by 64 publications

(68 citation statements)

References 52 publications

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“…Recently, we found that GM-CSF is a key molecule for cytokine induction by β-glucan, and that GM-CSF production is specifically induced in DBA/2 mice in vitro (12,14,15). In this study, we investigated the key factors in the cytokine induction by SCG in CY-treated mice, and found that GM-CSF plays an important role in this system.…”

Section: Discussionmentioning

confidence: 93%

“…Splenocytes were prepared as previously described (12). Briefly, the spleen was teased apart in RPMI 1640 medium, and after centrifugation, the single-cell suspension was treated with ACK-lysing buffer (8.29 g/liter NH 4 Cl, 1 g/liter KHCO 3 , 37.2 mg/liter EDTA/ 2Na) to lyse the red blood cells.…”

Section: Methodsmentioning

confidence: 99%

“…We previously reported that naive DBA/2 and DBA/1 mice show high sensitivity to SCG, and the splenocytes from these mice are potently stimulated by SCG to produce cytokines. Recently we found that GM-CSF is a key molecule for cytokine induction by β-glucan, and that GM-CSF is specifically induced by SCG in DBA/2 mice in vitro (12,14,15). In this study, we investigated the key factors in the hematopoietic response induced by SCG in CY-treated mice.…”

mentioning

confidence: 99%

“…DBA/2 and DBA/1 mice produce significantly higher titers of antibody to SCG than other inbred naive mice (13). Previously, we reported that interferon-(IFN-) γ, tumor necrosis factor-(TNF-) α interleukin-(IL-) 12p70 and granulocyte-macrophagecolony stimulating factor (GM-CSF) production was not induced by SCG in splenocytes derived from inbred strains of mice, except for DBA/1 and DBA/2 mice, and that GM-CSF was a key molecule among the induced cytokines (12,14,15). These data indicated that GM-CSF is a key molecule for cytokine induction by β-glucan, and that GM-CSF is specifically induced by SCG in DBA/2 mice in vitro.…”

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confidence: 99%

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Mechanism of Enhanced Hematopoietic Response by Soluble β‐Glucan SCG in Cyclophosphamide‐Treated Mice

Harada

Kawaminami

Miura

et al. 2006

Microbiology and Immunology

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Immunomodulating substances, biological response modifiers, and biotherapy, including immunotherapy, are important for the treatment of cancer. Some β-glucans are well-known biological response modifiers, and the mushrooms from which they are derived are widely distributed in nature and are used as medicine and food. Among the β-glucans, the 6-branched 1,3-β-glucan is the best characterized. Lentinan from Lentinus edodes (40) and Sonifilan (SPG) from Schizophyllum commune (9) have been used clinically for cancer therapy in Japan. We have analyzed the mechanism of β-glucanmediated immunopharmacological activity, and demonstrated the conformation-dependent and -independent activity of β-glucan (48, 49). Recent data indicated that orally administered β-1,3-glucan potentiated the activity of antitumor monoclonal antibody, leading to enhanced tumor regression and survival (20,21). Thus, cancer immunotherapy using β-glucan is a promising possibility.Sparassis crispa is a medicinal mushroom that recently became cultivatable in Japan. The primary component of the major cold NaOH-extracted polysaccharide fraction from S. crispa was found to be 6-branched 1,3-β-glucan, having one branch approximately every third main chain. This fraction showed strong antitumor activity against the solid form of Sarcoma 180 in ICR mice (34). In our recent study, we used S. crispa to treat several cancer patients in combination with lymphocyte transplantation immunotherapy and obtained a good response (35). To examine the pharmacological usefulness of S. crispa β-glucan Mechanism of Enhanced Hematopoietic

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Section: Discussionmentioning

confidence: 93%

Section: Methodsmentioning

confidence: 99%

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confidence: 99%

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Mechanism of Enhanced Hematopoietic Response by Soluble β‐Glucan SCG in Cyclophosphamide‐Treated Mice

Harada

Kawaminami

Miura

et al. 2006

Microbiology and Immunology

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“…Previously, we reported that both serum IgE levels and the scratching amount of NC/Nga mice that were induced dermatitis by a continuous application of hapten are reduced by oral administration of S. crispa (Hasegawa et al, 2004). Furthermore, a branched beta-glucan from S. crispa can induce IFN-γ in DBA/2 mice (Harada et al, 2002). However, the effects of oral administration of S. crispa on Th1/Th2 balance and allergic rhinitis have not yet been clarified.…”

Section: Introductionmentioning

confidence: 99%

Effects of Hanabiratake (Sparassis crispa) on Allergic Rhinitis in OVA-Sensitized Mice

Yao

Yamamoto

Kimura

et al. 2008

FSTR

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The anti-rhinitis properties of Sparassis crispa were investigated in mice. To examine the immunomodulative activity of oral administration of S. crispa, splenocytes obtained from ovalbumin-sensitized BALB/ c mice fed S. crispa were restimulated in vitro with the same antigen. Oral administration of S. crispa induced IFN-γ, but inhibited IL-4 and IL-5 secretion, and suppressed ovalbumin-specific IgE secretion by ovalbumin-stimulated splenocytes. The effects of S. crispa were further investigated by using the allergic rhinitis model in BALB/c mice. Nasal symptoms, sneezing and nasal rubbing induced by ovalbumin challenges were inhibited by oral administration of S. crispa in a dose-dependent manner. Furthermore, ovalbumin-specific serum IgE levels were diminished in this model. These results demonstrated that S. crispa may be effective in suppressing symptoms of allergic rhinitis through its immunomodulating activities.Keywords: allergic rhinitis, cytokines, hanabiratake, ovalbumin-specific IgE, Sparassis crispa *To whom correspondence should be addressed. Email: takashi-kimura@unitika.co.jp IntroductionType I allergies, including pollinosis, allergic rhinitis, atopic dermatitis and asthma, are characterized by an elevated production of IgE and mast cell degranulation that result in release of histamine as well as other chemical mediators of allergy (Platts-Mills, 2001). The production of IgE from B cells is regulated by T-helper (Th) cells, which have been classified into Th1 and Th2 subtype (Mosmann et al., 1986). Th2 cells synthesize IL-4, which enhances the IgE production by B cells through inducing IgE isotype class switching and the proliferation of Th2 cells (Le et al., 1990;Swain et al., 1990). Th2 cells also synthesize IL-5 which enhances the IL-4-dependent IgE production (Pene et al., 1988). Therefore, Th2 cells and IL-4 are considered to be critical for IgE production. Conversely, IFN-γ produced by Th1 cells suppresses IgE production both by interfering with the IL-4-derived isotype class switching (Thyphronitis et al., 1989) and by inhibiting the proliferation of Th2 cells (Gajewski and Fitch, 1988). IL-12 produced by antigen-presenting cells, such as dendritic cells and macrophages, is known to stimulate both NK and Th1 cells to make them produce IFN-γ. These two Th1-type cytokines, IL-12 and IFN-γ, enhance the proliferation of Th1 cells (Belosevic et al., 1989;Hsieh et al., 1993). It is generally accepted that enhancement of Th2-mediated immunity causes IgE-dependent allergic diseases. Therefore, properly regulating the balance between Th1-and Th2-type immune responses to heterogeneous antigens is considered to be an important mechanism in the prevention and therapy of the diseases mentioned earlier. In fact, it has been indicated that oral administration of some lactobacillus can modulate the host Th1/Th2 balance and decrease serum IgE levels (Fujiwara et al., 2004;Kimoto et al., 2004). Furthermore, oral administration of the extract from Hatakeshimeji (Lyophyllum decastes) mushroom can modulate t...

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Antitumor and immunomodulating effects of polysaccharides isolated from Solanum nigrum Linne

Gao

et al. 2009

Phytotherapy Research

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We have examined the effects of the crude polysaccharides isolated from Solanum nigrum Linne (SNL-P) in vitro and in vivo against U14 cervical cancer. SNL-P showed no antiproliferative effects in vitro at a dose up to 1 mg/ml. In vivo administration with SNL-P (90, 180, 360 mg/kg b.w., p.o.) decreased the number of ascites tumor cells and prolonged the survival time of U14 cervical-cancer-bearing mice. FACScan flow cytometer analysis showed that most of the ascites tumor cells were arrested in G2/M phase of cell cycle and the ratio of CD4+/CD8+ peripheral blood T-lymphocyte subpopulations were restored following treatment of SNL-P. Furthermore, the treatment with SNL-P also caused a significant increment in IFN-gamma (p < 0.01, 90, 180 and 360 mg/kg b.w.) and a remarkable decrease in Il-4 (p < 0.01, 90, 180 mg/kg b.w.; p < 0.05, 360 mg/kg b.w.) by the method of ELISA. These data showed that SNL-P possess potent antitumor activity and SNL-P might exert antitumor activity via activation of different immune responses in the host rather than by directly attacking cancer cells on the U14 cervical cancer bearing mice. Thus, SNL-P could be used as an immunomodulator and an anticancer agent.

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IFN-γInduction by SCG, 1,3-β-D-Glucan fromSparassis crispa, in DBA/2 MiceIn Vitro

Cited by 64 publications

References 52 publications

Mechanism of Enhanced Hematopoietic Response by Soluble β‐Glucan SCG in Cyclophosphamide‐Treated Mice

Mechanism of Enhanced Hematopoietic Response by Soluble β‐Glucan SCG in Cyclophosphamide‐Treated Mice

Effects of Hanabiratake (Sparassis crispa) on Allergic Rhinitis in OVA-Sensitized Mice

Antitumor and immunomodulating effects of polysaccharides isolated from Solanum nigrum Linne

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