IFN-τDisplays Anti-Inflammatory Effects onStaphylococcus aureusEndometritis via Inhibiting the Activation of the NF-κB and MAPK Pathways in Mice

Zhang, Zhenbiao; Guo, Yanxia; Liu, Yuzhu; Li, Chengye; Guo, Mengyao; Deng, Ganzhen

doi:10.1155/2017/2350482

Cited by 10 publications

(11 citation statements)

References 46 publications

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“…S. aureus is a common pathogen that can cause post-partum clinical and subclinical endometritis in humans and animals (Turner et al 2012;Zhang et al 2017). During the long-term use of antibiotics, S. aureus has been found to develop resistance (Hiramatsu 2001;Lai et al 2018;Pirolo et al 2019).…”

Section: Discussionmentioning

confidence: 99%

Therapeutic potential of a designed CSαβ peptide ID13 in Staphylococcus aureus-induced endometritis of mice

Yang

Shan

et al. 2020

Appl Microbiol Biotechnol

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Staphylococcus aureus is a common pathogen that can cause clinical and subclinical endometritis in humans and animals. In this study, a designed CSαβ peptide ID13 from DLP4 exhibited high stable antibacterial activity in simulated gastric fluid (90.79%), serum (99.54%), and different pH buffers (> 99%) against S. aureus CVCC 546 and lower cytotoxicity (89.62% viability) than its parent peptide DLP4 (74.14% viability) toward mouse endometrial epithelial cells (MEECs). ID13 caused a depolarization of bacterial membrane and downregulation of the expression of genes involved in membrane potential maintenance and biofilm formation. The in vitro efficacy analysis of ID13 showed a synergistic effect with vancomycin, ampicillin, rifampin, and ciprofloxacin; intracellular antimicrobial activity against S. aureus CVCC 546 in MEECs; and the ability to inhibit lipoteichoic acid-induced pro-inflammatory cytokines from RAW 264.7. In the S. aureus-induced endometritis of mice, similar to vancomycin, ID13 remarkably alleviated pathological conditions, inhibited the production of cytokines (TNF-α, IL-1ß, IL-6, and IL-10), and suppressed the TLR2-NF-κB signal pathway. Collectively, these results suggest that ID13 could be a potential candidate peptide for therapeutic application in S. aureus-induced endometritis. Key Points•Higher antibacterial activity and lower hemolysis of ID13 than DLP4. •ID13 could downregulate the genes of bacterial survival and infection.•ID13 could alleviate the S. aureus-induced endometritis of mice.•ID13 could regulate the cytokines and suppress the TLR2-NF-κB signal pathway.

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Section: Discussionmentioning

confidence: 99%

Therapeutic potential of a designed CSαβ peptide ID13 in Staphylococcus aureus-induced endometritis of mice

Yang

Shan

et al. 2020

Appl Microbiol Biotechnol

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“…Eventually, such recognition could elicit the initiation of the MyD88 pathway and then the downstream MAPKs and NF-κB, which is followed by release of diverse inflammation-associated cytokines such as TNF-α and IL-1β. Thus, involvement of these signaling pathways in the inflammatory system is possible ( Maisonneuve et al., 2014 ; Zhang et al., 2017 ; Qiao et al., 2020 ). According to the related studies, some LAB can achieve immunity modulation and inflammation mitigation via pathways like NF-κB and MAPKs ( Zhou et al., 2018 ; Mao et al., 2020 ; Yang et al., 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Lactobacillus rhamnosus GR-1 Alleviates Escherichia coli-Induced Inflammation via NF-κB and MAPKs Signaling in Bovine Endometrial Epithelial Cells

Liu

Feng

et al. 2022

Front. Cell. Infect. Microbiol.

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Escherichia coli counts as a major endometritis-causing pathogen among dairy cows, which lowered the economic benefits of dairy farming seriously. Probiotic consumption has been reported to impart beneficial effects on immunomodulation. However, the inflammatory regulation mechanism of probiotics on endometritis in dairy cows remains unexplored. The current work aimed to clarify the mechanism whereby Lactobacillus rhamnosus GR-1 (L. rhamnosus GR-1) resists bovine endometrial epithelial cells (BEECs) inflammatory injury induced by E. coli. The model of cellular inflammatory injury was established in the BEECs, which comes from the uterus of healthy dairy cows using E. coli. The outcome of L. rhamnosus GR-1 addition on inflammation was evaluated in BEECs with E. coli-induced endometritis. The underlying mechanisms of anti-inflammation by L. rhamnosus GR-1 were further explored in E. coli-stimulated BEECs. In accordance with the obtained results, the use L. rhamnosus GR-1 alone could not cause the change of inflammatory factors, while L. rhamnosus GR-1 could significantly alleviate the expression of E. coli-induced inflammatory factors. Based on further study, L. rhamnosus GR-1 significantly hindered the TLR4 and MyD88 expression stimulated by E. coli. Moreover, we observed that in BEECs, L. rhamnosus GR-1 could inhibit the E. coli-elicited expressions of pathway proteins that are associated with NF-κB and MAPKs. Briefly, L. rhamnosus GR-1 can effectively protect against E. coli-induced inflammatory response that may be closely related to the inhibition of TLR4 and MyD88 stimulating NF-κB and MAPKs.

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“…LPS is an exotoxin released by gram‐negative bacteria and is often used to induce inflammation (Kelly et al., 2020). When cells are stimulated by LPS, they activate inflammatory pathways, such as the Toll‐like receptor (TLR) pathway, NF‐κB signalling and MAPK signalling (Jhamat et al., 2020; Zhai et al., 2016; Zhang et al., 2017). Stimulating BEECs with LPS can induce an inflammatory response and release pro‐inflammatory cytokines, such as TNF‐α, IL‐1β and IL‐6 (Fu et al., 2015).…”

Section: Discussionmentioning

confidence: 99%

Aucubin ameliorates the LPS‐induced inflammatory response in bovine endometrial epithelial cells by inhibiting NF‐κB and activating the Keap1/Nrf2 signalling pathway

Gao

Feng

et al. 2021

Reprod Domestic Animals

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Bovine endometritis is the most common reproductive disease in the world and often causes infertility (Kasimanickam et al., 2006;Sheldon et al., 2019;Zhao et al., 2019a). Studies have shown that the incidence of bovine endometritis is as high as 40% and is gradually increasing every year (Carneiro et al., 2014;Wagener et al., 2017). Thus, this disease has seriously endangered the development of the breeding and dairy industries, causing tremendous economic losses worldwide (Marini et al., 2016; Nyabinwa et al., 2020; Swangchan-Uthai et al., 2012). The primary cause of bovine endometritis is bacterial infection after reproduction, which reduces the resistance of the endometrium, resulting in damage to the cow's endometrium. (Pothmann et al., 2015; Wathes, 2012). However, this disease is unavoidable in large-scale intensive breeding and breeding environments (Thompson et al., 2019). Previous studies have reported that pathogens such as Escherichia coli (E. coli), Staphylococcus aureus (S. aureus) and Trueperella pyogenes (T. pyogenes) have been isolated from the uterine tissues of sick cows, with E. coli being the most common (Ledgard et al., 2015; Piersanti et al., 2019). Lipopolysaccharide (LPS), an important component of the E. coli cell wall, is commonly used as a model for endometritis under

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IFN-τDisplays Anti-Inflammatory Effects onStaphylococcus aureusEndometritis via Inhibiting the Activation of the NF-κB and MAPK Pathways in Mice

Cited by 10 publications

References 46 publications

Therapeutic potential of a designed CSαβ peptide ID13 in Staphylococcus aureus-induced endometritis of mice

Therapeutic potential of a designed CSαβ peptide ID13 in Staphylococcus aureus-induced endometritis of mice

Lactobacillus rhamnosus GR-1 Alleviates Escherichia coli-Induced Inflammation via NF-κB and MAPKs Signaling in Bovine Endometrial Epithelial Cells

Aucubin ameliorates the LPS‐induced inflammatory response in bovine endometrial epithelial cells by inhibiting NF‐κB and activating the Keap1/Nrf2 signalling pathway

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