IgA response in mice infected with Neodiplostomum seoulensis

Abstract

To observe the production of IgA in Balb/c mice with neodiplostomiasis, 20 mice were infected with each 200 metacercariae of Neodiplostomum seoulenis. Sera and the duodenums were obtained 3, 7, 14, 28 days post-infection (PI) from five mice each group. Neodiplostomum specific IgA in serum by the enzyme-linked immunosorbent assay increased from 7 days PI and persisted till 28 days PI. Immunohistochemistry for IgA was done with sections of the duodenum. The IgA-positive reaction was generally seen in the lamina propria and submucosa. Some of epithelial cells were positive at 7 and 14 days PI. The present finding showed that Neodiplostomun specific IgA antibody increased in serum and that there was local reaction of IgA in the mucosa and submucosa of the duodenum but not directly related with worm expulsion.

Foodborne Intestinal Flukes in Southeast Asia

“…Serum and mucosal tissue IgA were increased after an experimental infection in mice, but the increase was not directly related to the worm expulsion [378]. Immunogold studies revealed that the tribocytic organ, seminal vesicle, caeca, and vitelline follicles were the major origins of worm antigens [379].…”

Antibody Responses in Sera of Different Mouse Strains Experimentally Infected with Neodiplostomum seoulense

“…Circulating and secretory IgA responses have been related to expulsion of intestinal trematodes and protozoans [20,21]. However, previous studies suggested that increases of IgA were not sufficient for worm rejection of N. seoulense and E. caproni [13,18]. In N. seoulsense infection, specific serum IgA antibodies were shown to increase from day 7 to 28 PI, and local IgA reactions were observed in the mucosa and submucosa of the duodenum, though not directly related with worm expulsion.…”