Both biotic and abiotic factors are important for maintaining the activity cycles of animals, and may determine the most advantageous periods for eating, moving and sleeping. While the solar day-night cycle is among the most central of abiotic variables, a number of species are active both day and night. Three-toed sloth populations (Bradypus, Linnaeus 1758) across the Neotropics show this pattern, with activity occurring at various points across the 24-hour cycle. In the current study, we used radio tracking techniques to investigate the daily temporal patterning of sloth displacement in Amazonian igapó flooded forest, and compared the results with populations studied in other habitats. We conclude that igapó sloths are cathemeral, and discussed that environmental temperature, nutritional needs and the presence of predators may be the main factors influencing their activity pattern.