IgE and IgG4 responses to shrimp allergen tropomyosin and its epitopes in patients from coastal areas of northern China

Abstract

Sensitization to allergens and their peptides varies among patients due to geographical or ethnic differences. The present study aimed to investigate immunoglobulin (ig)e and igG 4 responses to tropomyosin and its peptides in shrimp allergic patients from northern china. a total of 92 subjects were studied, including 35 shrimp allergic patients, 29 patients with house dust mite (HdM) and/or cockroach allergic patients and 28 healthy volunteers. Serum ige and igG 4 antibodies to recombinant shrimp tropomyosin (rPen a 1) and its peptides were measured by means of a light-initiated chemiluminescent assay. a total of 9 major sequential epitopes of Pen a 1 reported in the literature were synthesized. of 35 shrimp allergic patients, 25 (71.4%) had positive Pen 1-specific IgE (sige) antibodies and 22 (62.9%) contained measurable rPen a 1-specific igG 4 (sigG 4 ) antibodies. a strong igG 4 response accompanied the presence of ige to Pen a 1. none of the patients with HdM and/or cockroach allergy demonstrated ige reactivity to rPen a 1. The reaction frequency of ige binding epitope was 20-48%, while that of igG 4 binding epitope was 63.6-90.9%. The ige and igG 4 recognition patterns of the tropomyosin peptides demonstrated high interpatient heterogeneity. diversity of ige binding epitopes was positively correlated with Pen a 1 sige levels. in the study population, tropomyosin was a major allergen recognized by the majority of shrimp allergic patients, which is consistent with previous reports. However, none of the 9 epitopes are major (reaction frequency >50%) ige-binding regions, indicating the epitopes profile may be different in other regions.

Knowledge, Attitudes, and Practices of Patients with Allergic Rhinitis Towards Allergen-Specific Immunotherapy

“…These follow-up appointments also provide opportunities for healthcare professionals to reinforce treatment recommendations and educate patients on the importance of treatment adherence. 34 , 35 Patients should also be encouraged to actively seek knowledge and engage in self-management of their condition. Support groups and online forums can facilitate information exchange and provide a sense of community among patients.…”

The Heterogeneity of Ovomucoid-Specific IgE Idiotype Is Associated With Egg Allergy Symptom Severity

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Association between urban garbage exposure and allergic diseases among sanitation practitioners: A cross-sectional study

“…On the one hand, allergen-related IgG4 antibodies are thought to be involved in the development of clinical tolerance, and the mechanism of its immunoprotective effect is that IgG4 can compete with IgE for binding to allergen epitopes, thereby blocking IgE-mediated allergic reactions. 32 , 33 , 34 It has also been suggested that allergen-associated IgG is an incidental phenomenon and has nothing to do with function. 35 It is known that the incidence of food allergies such as egg and milk allergy in adults is much lower than that in children, and the serum IgG4 test results in this study showed that up to 50% of the individuals were positive for egg IgG4.…”