IgE antibodies to prohevein, hevein, and rubber elongation factor in children with latex allergy☆☆☆★★★

Ylitalo, Leea; Alenius, Harri; Turjanmaa, Kristiina; Palosuo, Timo; Reunala, Timo

doi:10.1016/s0091-6749(98)70284-2

Cited by 24 publications

(25 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hev b 1 is another latex allergen with a molecular mass of 14 kD that has been described as a major allergen [1, 2, 29]. In the present study, we show that the Hev b 1 content varies from one extract to another.…”

Section: Discussionsupporting

confidence: 58%

Interest of Two-Dimensional Electrophoretic Analysis for the Characterization of the Individual Sensitization to Latex Allergens

Chardin

Raulf‐Heimsoth

Chen

et al. 2002

Int Arch Allergy Immunol

View full text Add to dashboard Cite

Background/Objective: Latex allergy is a type 1 hypersensitivity reaction that mainly affects high-risk populations such as health care workers, spina bifida-affected or multiply-operated children. Ten molecules have so far been identified and registered as latex allergens (Hev b 1 to Hev b 10). The aim of the present investigation was to identify the major latex allergens by an individual analysis of the IgE response of latex-allergic patients to latex proteins separated by two-dimensional (2-D) gel electrophoresis. Materials and Methods: Latex proteins from a sap or a glove extract were separated by 2-D electrophoresis and transferred to a nitrocellulose membrane. Each membrane was incubated with the serum of one latex-allergic patient. The most frequently recognized latex allergens were characterized in sap and glove extracts using monoclonal antibodies or amino acid microsequencing. Results: The one-dimensional screening of 54 patient sera revealed 4 major bands recognized by IgE. The 2-D analysis of the sensitization to latex allergens allows the identification of allergen isoforms and the characterization of an individual response diversity. Hev b 6.01 was recognized by 88.9% of the patients. Protein spots around 14 kD were recognized by 48.1% of the patients and corresponded to Hev b 6.03 as well as other proteins. A not yet characterized doublet of acidic proteins with molecular masses of 43 and 94 kD was recognized by 20.4% of the sera. Only 5.5% of the sera did not recognize any of these 4 major allergens. Hev b 1 is the main protein from the glove extract but was not constantly found in sap extracts. Conclusions: One-dimensional electrophoretic analysis of the allergen is usually not sufficient to characterize the individual specificity of the IgE response to latex allergens. Latex-glove proteins which are allergens can be absent from the sap extracts and the sensitization to these allergens could be underestimated. Individual 2-D analysis of the sensitization to latex allergens is useful to define the best allergen mixture required for diagnosis and needed for individual therapy monitoring.

show abstract

Section: Discussionsupporting

confidence: 58%

Interest of Two-Dimensional Electrophoretic Analysis for the Characterization of the Individual Sensitization to Latex Allergens

Chardin

Raulf‐Heimsoth

Chen

et al. 2002

Int Arch Allergy Immunol

View full text Add to dashboard Cite

show abstract

“…Sera were obtained also from 35 NRL-allergic children, including seven children with spina bi®da or other congenital malformations (mean age 9K, range 1±18 years, mean number of surgical operations 12, range 5±21 operations) (17). All children met three diagnostic criteria for NRL allergy: 1) a positive SPT to a commercial NRL allergen (Stallerge Ánes SPT reagent, Antony, Cedex, France) (15) and/or to a NRL glove extract (Tri¯ex, Baxter) 2) a positive RAST (>0.4 kU/l; Pharmacia Biotech) 3) a positive NRL glove (Tri¯ex) challenge test performed as previously described (15).…”

Section: Methodsmentioning

confidence: 99%

IgE reactivity to patatin‐like latex allergen, Hev b 7, and to patatin of potato tuber, Sol t 1, in adults and children allergic to natural rubber latex

Seppälä

Palosuo

Kalkkinen

et al. 2000

Allergy

View full text Add to dashboard Cite

show abstract

“…For SB patients, prevalences up to 50% were obtained indicating that Hev b 6 was also an important allergen for those children. Ylitalo et al [35 ] tested nHev b 6.01 and nHev b 6.02 with sera of SB patients or other latex-allergic children with prevalences from 58 to 86%. Additional studies also revealed high prevalences for SB patients ranging from 30 to 69% [19, 20].…”

Section: Hev Bmentioning

confidence: 99%

<i>Hevea brasiliensis</i> Latex Allergens: Current Panel and Clinical Relevance

Wagner

Breiteneder²

2005

Int Arch Allergy Immunol

View full text Add to dashboard Cite

Latex allergy has been studied in detail in Europe and the US over the past two decades, resulting in specific guidelines that succeeded in reducing its incidence in high-risk populations within the medical field. How these developments have affected high-risk populations outside the health care scenario is an important unanswered question. In addition, a second wave of latex allergy may occur in nations that are striving to attain higher economic and technologic standards, including population-dense countries such as China. Therefore, the application of Hevea allergens in novel diagnostic assays and the development of specific latex immunotherapy will provide new opportunities for latex allergy research. In this review, we summarize current knowledge on the immunological properties of the 13 officially accepted Hevea brasiliensis latex allergens.

show abstract

IgE antibodies to prohevein, hevein, and rubber elongation factor in children with latex allergy☆☆☆★★★

Cited by 24 publications

References 22 publications

Interest of Two-Dimensional Electrophoretic Analysis for the Characterization of the Individual Sensitization to Latex Allergens

Interest of Two-Dimensional Electrophoretic Analysis for the Characterization of the Individual Sensitization to Latex Allergens

IgE reactivity to patatin‐like latex allergen, Hev b 7, and to patatin of potato tuber, Sol t 1, in adults and children allergic to natural rubber latex

<i>Hevea brasiliensis</i> Latex Allergens: Current Panel and Clinical Relevance

Contact Info

Product

Resources

About