IgE antibody to <i>Aspergillus fumigatus</i> recombinant allergens in cystic fibrosis patients with allergic bronchopulmonary aspergillosis

Knutsen, Alan P.; Hutcheson, Patricia S.; Slavin, Raymond G.; Kurup, Viswanath P.

doi:10.1046/j.1398-9995.2003.00310.x

Cited by 76 publications

(63 citation statements)

References 12 publications

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“…Other authors (11) have reported that rAspf3 and rAspf4 allow discrimination between ABPA and non-ABPA patients with atopic A. fumigatus-positive skin tests. According to the data in the literature (2,11,21), rAspf4 (a cytoplasmic protein with unknown function) and rAspf6 recombinants have provided powerful tools for the accurate diagnosis of ABPA. Kraemer et al (13) have shown that allergen-specific IgE against these two recombinants could be detected almost exclusively in the sera of patients with ABPA.…”

Section: Discussionmentioning

confidence: 99%

Recombinant Allergens Combined with Biological Markers in the Diagnosis of Allergic Bronchopulmonary Aspergillosis in Cystic Fibrosis Patients

Fricker‐Hidalgo

Coltey

Llerena

et al. 2010

Clin Vaccine Immunol

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Allergic bronchopulmonary aspergillosis (ABPA) is a frequent complication in cystic fibrosis patients. The diagnosis remains difficult and requires a combination of clinical, radiological, biological, and mycological criteria. The aim of this study was to analyze the added value of two recombinant antigens, rAspf4 and rAspf6, associated with the detection of specific IgG; precipitins; total IgE; and Aspergillus fumigatus in sputum for the diagnosis of ABPA. In a retrospective study, we determined the specific IgE responses to these recombinants in 133 sera of 65 cystic fibrosis patients. We selected an average of five serum samples from each of the 17 patients with ABPA (13 proven and 4 probable ABPA) and from 3 patients with Aspergillus bronchitis and rhinosinusitis. One serum sample for the 45 patients without ABPA was tested. The sensitivity of specific IgE detection against rAspf4 calculated per patient (92.3%) was significantly higher (P < 0.05) than that of rAspf6 (53.8%). When rAspf4 IgE detection was associated with anti-Aspergillus IgG enzyme-linked immunosorbent assay (ELISA) and precipitin detection, the sensitivity rose to 100%. The specificities of rAspf4 and rAspf6 IgE detection were 93.7% and 91.6%, respectively. Other diagnostic criteria had slightly lower specificities (87.5% for anti-Aspergillus IgG ELISA, 89.6% for precipitins, 84.4% for total IgE, and 85.0% for positive A. fumigatus culture in sputum). In conclusion, this retrospective study showed the relevance of rAspf4 IgE detection, in combination with other biological markers (Aspergillus IgG ELISA, precipitins, and total IgE), for improving the biological diagnosis of ABPA.

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Section: Discussionmentioning

confidence: 99%

Recombinant Allergens Combined with Biological Markers in the Diagnosis of Allergic Bronchopulmonary Aspergillosis in Cystic Fibrosis Patients

Fricker‐Hidalgo

Coltey

Llerena

et al. 2010

Clin Vaccine Immunol

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“…Additionally, specific IgE against rAsp f were used. The development of antibodies to recombinant allergens has recently been reported [5,12,15] and shows an improvement in diagnosis as well as the capacity to distinguish between ABPA patients (even during remission) and AFS patients.…”

Section: Discussionmentioning

confidence: 99%

Risk factors for allergic bronchopulmonary aspergillosis and sensitisation to Aspergillus fumigatus in patients with cystic fibrosis

et al. 2005

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Cystic fibrosis patients with allergic bronchopulmonary aspergillosis have a more frequent isolation of S. maltophilia in their sputum than their controls. Longer duration of colonisation with P. aeruginosa is a risk factor for Aspergillus fumigatus sensitisation. Higher cumulative doses of inhaled corticosteroids are associated with Aspergillus fumigatus sensitisation and their role as a risk factor needs to be clarified.

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“…The DCs form an immune sensory mesh surrounding the airway, rapidly taking up and processing antigens from the environment; they do this by extending processes through intercellular spaces in the epithelium and sampling the airway milieu, a mechanism described as "snorkeling" [28,29]. The increased exposure and activation of DCs could explain the increased prevalence of adaptive T-cell responses such as allergic bronchopulmonary aspergillosis (ABPA) in CF patients [30]. Thus the effect of CFTR and the ASL in regulating antigen uptake in the lung is an area of lung immunology which needs to be explored (Fig.…”

Section: Innate Immunity In Cfmentioning

confidence: 99%

Is cystic fibrosis a TH17 disease?

2007

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Cystic Fibrosis (CF) is the most common lethal genetic disease in the Caucasian population and typically results in the development of bronchial inflammation, bronchiectasis, the progressive loss of lung function and ultimately death. Recently it has been shown that products of the Th(17) subset of T-cells, specifically, IL-17A and IL-17F are elevated in the sputum of CF patients. This review will go over experimental evidence supporting a role for the IL- 23/IL-17 axis in CF lung inflammation.

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IgE antibody to Aspergillus fumigatus recombinant allergens in cystic fibrosis patients with allergic bronchopulmonary aspergillosis

Cited by 76 publications

References 12 publications

Recombinant Allergens Combined with Biological Markers in the Diagnosis of Allergic Bronchopulmonary Aspergillosis in Cystic Fibrosis Patients

Recombinant Allergens Combined with Biological Markers in the Diagnosis of Allergic Bronchopulmonary Aspergillosis in Cystic Fibrosis Patients

Risk factors for allergic bronchopulmonary aspergillosis and sensitisation to Aspergillus fumigatus in patients with cystic fibrosis

Is cystic fibrosis a TH17 disease?

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