IgE-based assay for early detection of HIV-1 infection in infants

Míguez‐Burbano, Maria José; Hutto, Cecelia; Shor‐Posner, Gail; Scott, Gwendolyn B.; Lopez, Viola; Lai, Shenghan; Li, Hong; Fletcher, MaryAnn; Baum, Marianna K.

doi:10.1016/s0140-6736(05)62566-4

Cited by 8 publications

(12 citation statements)

References 4 publications

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“…The IgE response is rapid and reaches a peak earlier than the IgG antibodies, suggesting that an IgE-based assay may detect seroconversion earlier than the conventional method. The high sensitivity of the IgE assay is in accord with other reports showing that immunoassays based on IgE antibodies directed to infectious agents are at least as specific and sensitive as those based on other immunoglobulin antibody responses (1,14,15,16,18,22,26). Of particular advantage to laboratories in developing countries, the IgE test can be rapidly performed without complex laboratory equipment and can be run at room temperature, and the same technology may be used for both children and adults (14).…”

Section: Discussionsupporting

confidence: 79%

“…Since maternal IgE does not cross the placenta and IgE is produced before any other immunoglobulin in the fetus and in adults, an IgE-based assay may be of particular importance in providing early detection of HIV-1 infection in infants (14) and acute infection in adults.…”

Section: Discussionmentioning

confidence: 99%

“…The potential advantage of an IgE antibody test in HIV disease is supported by previous findings demonstrating specific IgE directed to infectious agents. Certain viral infections are known to produce specific IgE antibodies, to the extent that significant changes in the level of total serum IgE may occur (1,14,15,16,18,20,21,22,26). Of importance, during the early stages of HIV-1 disease a significant elevation of total IgE has been reported in children (7,24,25).…”

mentioning

confidence: 99%

“…In a simultaneous investigation, we evaluated the presence of IgEspecific antibody to HIV and performed an IgE-based assay for early detection of HIV-1 infection in infants and young children (14).…”

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confidence: 99%

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Diagnosis of Human Immunodeficiency Virus Infection Using an Immunoglobulin E-Based Assay

Fletcher¹,

Míguez‐Burbano

Shor‐Posner

et al. 2000

Clin Diagn Lab Immunol

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Immunoglobulin assays that are sensitive and specific for detecting human immunodeficiency virus type 1 (HIV-1) infection are especially important in developing countries where PCR and viral culture may not be readily available. Immunoglobulin E (IgE), which is elevated in HIV-1 infection, is the only antibody that does not cross the placenta, making it potentially valuable for viral detection in both children and adults. This study developed an assay for detection of HIV specific IgE antibodies in adults. A total of 170 serum samples from 170 adults (116 HIV positive and 54 HIV negative) were analyzed. Serum or plasma samples were treated by using the protein G affinity method. The HIV status was determined by using two IgG enzyme-linked immunosorbent assays (ELISAs) and one Western blot evaluation. The IgE enzyme immunoassay test for HIV-1 correctly identified the HIV status in 98.8% of the samples (168 of 170). One false-positive and one falsenegative test occurred with the IgE ELISA, as well as with the IgG ELISA test but were correctly identified by the IgE test. Analysis of the data demonstrated a high specificity (99%) and sensitivity (99%) of the IgE test, with 95% confidence intervals. The IgE assay appears to be sensitive and specific, suggesting that IgE-specific antibodies offer an effective method to detect HIV-1 infection in adults.

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Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Diagnosis of Human Immunodeficiency Virus Infection Using an Immunoglobulin E-Based Assay

Fletcher¹,

Míguez‐Burbano

Shor‐Posner

et al. 2000

Clin Diagn Lab Immunol

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“…24,25 It has been suggested that HIV-specific IgE in cord blood could be used as an indicator of infection in the infant under the assumption that such IgE is the result of intrauterine production. 25 However, the current study shows that such IgE is likely to be the result of materno-fetal transfer, and we suggest that future studies on specific IgE in cord blood should focus on studying and excluding such materno-fetal transfer of IgE.…”

Section: Discussionmentioning

confidence: 99%

Sensitization does not develop in utero

Bønnelykke

Pipper

Bisgaard

2008

Journal of Allergy and Clinical Immunology

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Problems in Diagnosis of HIV Infection in Babies

Praharaj¹

2006

Medical Journal Armed Forces India

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Serological diagnosis of human immunodeficiency virus (HIV) infection in babies born to HIV infected mothers is difficult because of presence of maternal anti-HIV antibody up to 18 months. Conventional enzyme-linked immunosorbent assay (ELISA) and western blot may be positive in un-infected cases. Various other modalities which have been adopted include detection of HIV specific IgA, IgM, IgE, detection of p24 antigen, viral culture and detection of HIV nucleic acid by polymerase chain reaction (PCR). Viral culture or PCR positivity within first 48 hours of life indicates intrauterine infection. An early diagnosis of HIV infection in babies born to HIV infected mothers is essential as definite antiretroviral therapy (ART) can be instituted and unnecessary toxicity of drug therapy avoided if found negative. Though viral culture and DNA-PCR has sensitivity of >95% after one month of age, some cases can not be diagnosed during this period. Other tests like viral RNA detection by reverse transcription polymerase chain reaction (RT-PCR) and combination of tests will be required.

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IgE-based assay for early detection of HIV-1 infection in infants

Cited by 8 publications

References 4 publications

Diagnosis of Human Immunodeficiency Virus Infection Using an Immunoglobulin E-Based Assay

Diagnosis of Human Immunodeficiency Virus Infection Using an Immunoglobulin E-Based Assay

Sensitization does not develop in utero

Problems in Diagnosis of HIV Infection in Babies

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