IgE production in atopic patients is not related to IL-4 production

Abstract

SUMMARYTo analy.se whether there is a general defect in T or B cell function in atopic individuals we have measured cytokine and IgE production by peripheral blood lymphocytes, isolated from 19 atopic donors (17 asthma/rhinitis and two dermatitis patients) in comparison with 19 non-atopic controls. After stimulation oflymphocytes with aiiti-CD2 and anti-CD28. we found no significant difference in IL-2, IL-4 and interferon-gamma (IEN--)) production. To examine the correlation between the production of IgE and IL-4, we stimulated lymphocytes with anti-CD2 and rIL-2. Under this condition both T eell iL-4 and B cell IgE production ean be measured. No significant diflerence was found for the amount of IgE and IL-4 produced between the two groups ( P > 005). The non-atopie donors showed a good correlation between IL-4 and IgE production (r = 0'70). Surprisingly, within the atopic group there was no correlation between IgE and IL-4 production at all (r = -0-04). The ratio of IgE to IL-4 was higher (although not signifieatitly) in the atopic group. Our data suggest that in atopic donors IgE production is less dependent on IL-4, and that other cytokines are involved.

Topical Azithromycin and Clarithromycin Inhibit Acute and Chronic Skin Inflammation in Sensitized Mice, with Apparent Selectivity for Th2-Mediated Processes in Delayed-Type Hypersensitivity

“…11a). In human atopic dermatitis, with which murine chronic OXA-DTH bears some resemblance [25], high IgE titres are not necessarily totally dependent on IL-4 [37]. Therefore, it is possible that clarithromycin reduced serum IgE concentration by IL-4-independent mechanisms.…”

B7‐2/CD28 costimulatory pathway in children with atopic dermatitis and its connection with immunoglobulin E, intracellular interleukin‐4 and interferon‐gamma production by T cells during a 1‐month follow‐up

“…We also found extremely poor correlation between IgE and IL-4 production as previously shown by van der Pouw Kraan et al . 30 and surprisingly found correlation of IFN-γ producing T helper cells with total serum IgE in children with AD.…”

Interleukin-4 and interferon-γ are possible allergic markers in pediatric patients with β-lactam hypersensitivity

“…[24] At present, it is well established that cytokines play an important role in many drug-mediated hypersensitivity reactions, ranging from mild rashes to life-threatening reactions. [78910111213141524] However, the potential for specific cytokine (s) to elicit hypersensitivity responses or to contribute to allergic pathogenesis remains largely unexplored in human. Therefore, in this study, we have made an attempt to investigate the role of cytokines in the pathogenesis of pediatric patients which showed hypersensitivity against β-lactam agents.…”

“…[710] In many studies, interferon (IFN)-γ production was reported to be essentially normal in atopic subjects,[11] whereas in others, it was markedly reduced[12] or elevated. [13] Interleukin (IL)-4 production was usually[14] but not always[15] reported as substantially higher. In some studies, neither IL-4 nor IFN-γ production was different,[716] whereas in others, synthesis of both cytokines was affected, with IFN-γ inhibited and IL-4 elevated[717] relative to the responses elicited in normal controls.…”

“…Some of these discrepancies may reflect different etiologies for related but distinct hypersensitivity states, i.e., atopic dermatitis versus allergic rhinitis. [71011121314151617] However, the variation frequently observed by different groups studying the same disease may also stem from the fact that many studies have been carried out with very small numbers of subjects. [7] Despite the availability of modern approaches, these discrepancies in cytokines productions under allergic disorders have not been fully clarified.…”