IgE-reactivity profiles to nonspecific lipid transfer proteins in a northwestern European country

Faber, Margaretha A.; Gasse, Athina L. Van; Decuyper, Ine I.; Uyttebroek, Astrid P.; Sabato, Vito; Hagendorens, Margo M.; Bridts, Chris H.; Clerck, Luc S. De; Fernández‐Rivas, Montserrat; Pascal, Mariona; Díaz‐Perales, Araceli; Ebo, Didier G.

doi:10.1016/j.jaci.2016.06.016

Cited by 42 publications

(62 citation statements)

References 11 publications

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“…In our own casecontrol study, sensitization to ns-LTP was demonstrable in 10 of 12 (83%) patients with cannabis-related food allergies (8). In comparison, in our regions sIgE reactivity towards ns-LTP in patients with pollen and/or plant-derived food allergies is observed in about 25% of the cases (28). Anyhow, it appears that next to peach (24) and mugwort (29) C. Sativa might be another source that dominates sensitization to ns-LTP and triggers ns-LTP-related allergies that extend far beyond vegetables and fruits.…”

Section: Allergenic Componentscontrasting

confidence: 41%

Cannabis sativaallergy: looking through the fog

Decuyper

Gasse

Cop

et al. 2016

Allergy

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IgE-mediated Cannabis (C. sativa, marihuana) allergy seems to be on the rise. Both active and passive exposure to cannabis allergens may trigger a C. sativa sensitization and/or allergy. The clinical presentation of a C. sativa allergy varies from mild to life-threatening reactions and often seems to depend on the route of exposure. In addition, sensitization to cannabis allergens can result in various cross-allergies, mostly for plant foods. This clinical entity, designated as the 'cannabis-fruit/vegetable syndrome', might also imply cross-reactivity with tobacco, natural latex and plant-food-derived alcoholic beverages. Hitherto, these crossallergies are predominantly reported in Europe and appear mainly to rely upon cross-reactivity between nonspecific lipid transfer proteins or thaumatin-like proteins present in C. sativa and their homologues, ubiquitously distributed throughout plant kingdom. At present, diagnosis of cannabis-related allergies predominantly rests upon a thorough history completed with skin testing using native extracts from crushed buds and leaves. However, quantification of specific IgE antibodies and basophil activation tests can also be helpful to establish correct diagnosis. In the absence of a cure, treatment comprises absolute avoidance measures. Whether avoidance of further use will halt the extension of related cross-allergies remains uncertain.Cannabis sativa (C. sativa) is an annual, dioecious and anemophilous flowering plant (order: Rosales, family Cannabaceae) that is native to central and southern Asia and the Caucasian region. Different preparations [dried flowering tops, hashish, hashish oil] are obtained from cannabis varieties containing elevated levels of cannabinoids, especially delta-9-tetrahydrocannabinol (THC), several of them being more or less potent psychoactive substances. Although, today, cannabis use is still illegal in most countries, it is widespread for its relaxing and euphoric effects. Furthermore, worldwide the illegal status of the drug has gained more and more resistance recently, resulting in legalization of both sale and possession of marijuana in Colorado, Alaska, Oregan and Washington, for example, both for medicinal and recreational use (1). Different European countries are debating the legalization of cannabis as well. While consumption of cannabis has been legal under certain conditions for a while

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Section: Allergenic Componentscontrasting

confidence: 41%

Cannabis sativaallergy: looking through the fog

Decuyper

Gasse

Cop

et al. 2016

Allergy

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“…Thus, the BAT might be useful if clinical history, SPT and/or sIgE measurement are inconsistent or in addition an oral food challenge is not possible [42]. Furthermore, the use of recombinant allergens in the BAT might have added value in discriminating between clinically relevant and mere sensitization [44, 45] and should be considered in future studies.…”

Section: Discussionmentioning

confidence: 99%

Exploratory analysis of CD63 and CD203c expression in basophils from hazelnut sensitized and allergic individuals

Lötzsch

Dölle

Vieths

et al. 2016

Clin Transl Allergy

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BackgroundSensitization to hazelnut (HN) is frequent and requires clarification to determine whether this sensitization is clinically relevant. The aim of this study was to investigate basophil activation profiles in HN-sensitized and allergic subjects.MethodsBasophil activation was determined by flow cytometric analyses of CD63 and CD203c expression using several HN allergen concentrations. Depending on their clinical reaction pattern, an oral allergy symptom group (OAS, n = 20), a systemic reaction group (n = 12) and a sensitized group without clinical symptoms (n = 20) were identified. Additionally, 10 non-allergic and non-sensitized individuals served as controls.ResultsCD63 and CD203c expression differed between allergic (OAS and systemic group) and sensitized subjects. The HN concentration required to activate 30% of CD203c+ basophils [effective concentration (EC)30] was significantly higher in sensitized versus the allergic group (p = 0.0089). This was more pronounced when the basophil allergen threshold sensitivity (CD-sens) was calculated (CD63: p = 0.018; CD203c: p = 0.009).ConclusionOur data indicate that the basophil activation test may provide information to better distinguish between sensitized and allergic subjects if several allergen concentrations are considered. CD203c expression displayed a better discrimination compared to CD63; therefore, its diagnostic value might be superior compared with CD63.Electronic supplementary materialThe online version of this article (doi:10.1186/s13601-016-0134-7) contains supplementary material, which is available to authorized users.

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“…In food allergy, it has been demonstrated that both the basophil activation test (BAT) and quantification of sIgG4 antibodies can be helpful in differentiating clinically relevant from irrelevant sIgE results . Preliminary results of our earlier study showed that the BAT with Pru p 3 has good potential in predicting clinical reactivity to peach in an Antwerp population . The diagnostic usefulness of sIgG4 to Pru p 3/Mal d 3 has not been explored yet.…”

Section: Introductionmentioning

confidence: 99%

Performance of basophil activation test and specific IgG4 as diagnostic tools in nonspecific lipid transfer protein allergy: Antwerp‐Barcelona comparison

Decuyper

Pascal

Gasse

et al. 2019

Allergy

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Background Recent studies show that nsLTP sensitization is not limited to the Mediterranean basin and can present diverse clinical phenotypes. It remains challenging to predict clinical outcome when specific IgE antibodies (sIgE) to nsLTPs are present. This study compares both clinical and in vitro allergy characteristics but also diagnostic performance of a basophil activation test (BAT) and sIgG4 in nsLTP‐sensitized patients from Antwerp (ANT, Belgium) and Barcelona (BCN, Spain). Methods Adult subjects with positive sIgE rPru p 3 and/or rMal d 3 ≥ 0.10 kUA/L (n = 182) and healthy controls (n = 37) were included. NsLTP‐sensitized individuals were stratified according to clinical symptoms with peach/apple, respectively. BAT rPru p 3 and rMal d 3 were performed and sIgG4 antibodies to both components quantified. Results In BCN, only ratios of sIgG4/sIgE rMal d 3 and BAT rMal d 3 (0.001 µg/mL) can identify clinically relevant Mal d 3 sensitization (sensitivity of 60%‐63% and a specificity of 75%‐67%, respectively). In ANT, only the sIgE/total IgE rPru p 3 ratio shows added value (sensitivity 60% and specificity 83%). Finally, it appears that symptomatic patients in BCN are more sensitive to lower allergen concentrations compared to ANT. In addition, it was shown that ANT patients were more often sensitized to pollen and that specific pollen sources differed between regions. Conclusions NsLTP‐related allergy profiles and diagnostic performance differ significantly between regions and are component‐specific, which makes extrapolation of data difficult to do. In addition, it seems that basophil sensitivity might show geographical differences. Additional research is needed to confirm these findings.

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IgE-reactivity profiles to nonspecific lipid transfer proteins in a northwestern European country

Cited by 42 publications

References 11 publications

Cannabis sativaallergy: looking through the fog

Cannabis sativaallergy: looking through the fog

Exploratory analysis of CD63 and CD203c expression in basophils from hazelnut sensitized and allergic individuals

Performance of basophil activation test and specific IgG4 as diagnostic tools in nonspecific lipid transfer protein allergy: Antwerp‐Barcelona comparison

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