IGF2BP2, an RNA‐binding protein regulates cell proliferation and osteogenic differentiation by stabilizing <i>SRF</i> mRNA

Zhou, Zimo; Chen, Senxiang; Wu, Tong; Chen, Yifeng; Cao, Yue; Huang, Ying; Li, Da

doi:10.1002/jcp.30919

Cited by 11 publications

(2 citation statements)

References 42 publications

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“…Zhang et al [ 49 ] found that IGF2BP2 targets and stabilizes serum response factor ( SRF ) mRNA, regulates osteogenic differentiation (OGD), and can be a potential marker and therapeutic target for osteoporosis and OGD. Although the role of IGF2BP2 in cancer has been extensively studied, the mechanism by which it regulates cell proliferation and differentiation remains unclear.…”

Section: Discussionmentioning

confidence: 99%

Lin28 affects the proliferation and osteogenic differentiation of human dental pulp stem cells by directly inhibiting let-7b maturation

Yan,

Sun,

Wang

et al. 2024

BDJ Open

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Objective Activation of Lin28 gene under certain conditions promotes tissue damage repair. However, it remains unknown whether conditional expression of Lin28 facilitates the recovery of damaged pulp tissue. In the study, we focus on exploring the effects and possible regulatory mechanisms of Lin28 on the proliferation and differentiation of human dental pulp stem cells (hDPSCs). Materials and methods We adopted techniques such as the ethynyl-2ʹ-deoxyuridine (EdU) incorporation assay, RNA-protein immunoprecipitation (RIP) analysis, and luciferase assays to study the regulation of hDPSCs by Lin28. Furthermore, gain-of-function and loss-of-function analyses were also used in explored factors regulating hDPSCs activation. Results The results show that Lin28 inhibited osteogenic differentiation by directly targets pre-let-7b. Through bioinformatics sequencing and dual luciferase experiments we learned that let-7b directly targets the IGF2BP2 3’UTR. Silencing of IGF2BP2 showed a similar biological effect as overexpression of let-7b. Overexpression of IGF2BP2 counteracted the differentiation-promoting effects produced by let-7b overexpression. Discussion/conclusions In conclusion, the RNA-binding protein Lin28 regulates osteogenic differentiation of hDPSCs by inhibiting let-7 miRNA maturation. And mature let-7b directly regulated the expression of IGF2BP2 by targeting the 3’UTR region of IGF2BP2 mRNA thus further inhibiting the differentiation of hDPSCs.

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Section: Discussionmentioning

confidence: 99%

Lin28 affects the proliferation and osteogenic differentiation of human dental pulp stem cells by directly inhibiting let-7b maturation

Yan,

Sun,

Wang

et al. 2024

BDJ Open

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“…Insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2), an RNA-binding protein (RBP), plays an important role in cell growth and human disease progression [ 8 , 9 ]. As an N6-methyladenosine (m6A) reader, IGF2BP2 is involved in cancer progression by binding to different RNAs [ 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

m6A reader IGF2BP2 promotes M2 macrophage polarization and malignant biological behavior of bladder cancer by stabilizing NRP1 mRNA expression

Fu,

Shi,

et al. 2024

BMC Urol

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Background Insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2) has been confirmed to play oncogenic role in many cancers. However, the role and mechanism of IGF2BP2 in bladder cancer (BCa) still deserves to be further revealed. Methods The mRNA and protein levels of IGF2BP2 and neuronilin-1 (NRP1) were detected by real-time quantitative PCR (RT-qPCR) and western blot. Cell proliferation, apoptosis, migration and invasion were determined using colony formation assay, EdU assay, CCK8 assay, flow cytometry and transwell assay. Xenograft tumor model was conducted to evaluate the role of IGF2BP2 in vivo. THP-1-M0 macrophages were co-cultured with the condition medium (CM) of BCa cells to induce polarization. M2 macrophage polarization was assessed by detecting the mRNA levels of M2 macrophage markers using RT-qPCR and measuring the proportion of M2 macrophage markers using flow cytometry. Moreover, MeRIP and RIP assay were performed to assess m6A level and the interaction between IGF2BP2 and NRP1. Results IGF2BP2 and NRP1 were upregulated in BCa tissues and cells. IGF2BP2 knockdown suppressed BCa cell growth and metastasis, as well as inhibited BCa tumor growth. After THP-1-M0 macrophages were co-cultured with the CM of BCa cells, the levels of M2 macrophage markers were markedly enhanced, while this effect was abolished by IGF2BP2 knockdown. IGF2BP2 level was positively correlated with NRP1 level, and it could increase NRP1 mRNA stability. NRP1 overexpression reversed the suppressive effect of IGF2BP2 knockdown on M2 macrophage polarization and BCa cell progression. Conclusion m6A-reader IGF2BP2 enhanced M2 macrophage polarization and BCa cell progression by promoting NRP1 mRNA stability.

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Effect of RNA-binding proteins on osteogenic differentiation of bone marrow mesenchymal stem cells

Luo

Jiang

2023

Mol Cell Biochem

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IGF2BP2, an RNA‐binding protein regulates cell proliferation and osteogenic differentiation by stabilizing SRF mRNA

Cited by 11 publications

References 42 publications

Lin28 affects the proliferation and osteogenic differentiation of human dental pulp stem cells by directly inhibiting let-7b maturation

Lin28 affects the proliferation and osteogenic differentiation of human dental pulp stem cells by directly inhibiting let-7b maturation

m6A reader IGF2BP2 promotes M2 macrophage polarization and malignant biological behavior of bladder cancer by stabilizing NRP1 mRNA expression

Effect of RNA-binding proteins on osteogenic differentiation of bone marrow mesenchymal stem cells

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