IgG and IgA subclass mRNA-bearing plasma cells in periodontitis gingival tissue and immunoglobulin levels in the gingival crevicular fluid

Abstract

SUMMARYThe humoral immune response, especially the production of IgG and IgA, is considered to have a protective role in the pathogenesis of periodontal disease, but the precise mechanisms are still unknown. In order to determine local IgG and IgA production, we investigated the presence of human IgG and IgA subclass mRNA-bearing plasma cells within periodontal tissue by in situ hybridization using digoxigenin-labelled oligonucleotide probes in 24 gingival biopsy samples (pocket depth 5 5 mm) which were obtained from eight patients with adult periodontitis. Furthermore, we examined IgG and IgA subclass proteins and digested IgA1 Fab portions in the gingival crevicular fluid (GCF), corresponding to the sites from which the tissues were taken, by ELISA. IgG and IgA subclass mRNA-expressing cells were detected in all serial formalin-fixed/paraffinembedded gingival tissue sections sampled. Plasma cells showed strong cytoplasmic staining with a high contrast and a good retention of morphology with these probes. IgG1 mRNA-expressing cells were predominant (mean 63%) and IgG2 mRNA-expressing cells were present at around 23% of total IgG plasma cells, while IgG3 and IgG4 mRNA-expressing cells were present to a much lesser extent (3% and 10%, respectively). Similar proportions of IgG subclass proteins in GCF were detected, which were also consistent with 'normal' serum levels. In terms of IgA subclass, IgA1 mRNA-positive cells were predominant (mean 65 . 1%, P

The potential role of interleukin‐17 in the immunopathology of periodontal disease

“…(1) IL-17 produced by activated T cells in periodontal lesions is bound to IL-17 receptor-positive cells, (2) IL-17 may be degraded in the gingival crevice, as is known to occur for IgA1 (Takahashi et al 1997), by both bacteria-and host cellderived proteases, and (3) the amount of IL-17 in GCF is relatively low. Our results suggest that IL-17 produced by gingival T cells may be bound by membrane-bound IL-17 receptors in periodontal tissues.…”

The systemic immune response is more prominent than the mucosal immune response in the pathogenesis of periodontal disease

“…The slides were immersed in prehybridization buffer (×2 SSC and 50% formamide) for 2 h at 37°C. Hybridization mixtures for the probes were prepared and hybridization was performed as described in a previous study (Takahashi et al 1997b). As a negative control, sections were incubated with hybridization buffer only or with antisense probes.…”

The Interleukin‐1 and Fcγ Receptor Gene Polymorphisms in Japanese Patients With Rheumatoid Arthritis and Periodontitis

“…RA is characterized by the presence of rheumatoid factors that are autoantibodies directed to the constant region of immunoglobulin G (IgG) in the joints. Likewise, a strong IgG response specific for periodontopathic bacteria was observed in the periodontitis lesions 14,15 . The inflammatory cascade induced by IgG‐containing ICs is initiated by IgG Fc receptor (FcγR) on phagocytes.…”