IgG Autoantibodies Against IgE from Atopic Dermatitis Can Induce the Release of Cytokines and Proinflammatory Mediators from Basophils and Mast Cells

Poto, Remo; Quinti, Isabella; Marone, Gianni; Taglialatela, Maurizio; Paulis, Amato de; Casolaro, Vincenzo; Varricchi, Gilda

doi:10.3389/fimmu.2022.880412

Cited by 16 publications

(17 citation statements)

References 105 publications

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“…Mast cells activated in atopic dermatitis release a large number of inflammatory cytokines, histamine, and protease through degranulation [ 7 , 8 ] and play an important role in regulating inflammatory response and itching [ 9 , 10 ]. Mas-related G protein-coupled receptor-X2 (MRGPRX2) is specifically expressed by skin mast cells and is considered a new marker for regulating mast cell degranulation, chronic inflammation, and anaphylaxis [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Inhibition of Mast Cell Degranulation in Atopic Dermatitis by Celastrol through Suppressing MRGPRX2

Yao

Chen

2023

Disease Markers

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Background. Atopic dermatitis is a common dermatological disease, and mast cell degranulation is believed to be related with the progression of atopic dermatitis. Mas-related G protein-coupled receptor-X2 (MRGPRX2), and calcium release-activated calcium channel protein 1-2 (ORAI-1, ORAI-2) are involved in mast cell degranulation. Celastrol is an active monomer of Tripterygium wilfordii, and it presents an antiatopic role. Methods. 2,4-Dinitrofluorobenzene (DNFB) and compound 48/80 (C 48/80) were used to establish a slow and acute scratching animal model, respectively. Hematoxylin-eosin and toluidine blue staining was used to investigate tissue injury. Inflammatory factor concentration was measured with ELISA. The expression of MRGPRX2, ORAI-1, and ORAI-2 was detected with immunohistochemistry (IHC) staining. Gene expression profiling and microRNA array were performed to investigate gene differential expression. Results. Celastrol greatly inhibited atopic dermatitis-related tissues injury, mast cell production, histamine release, scratching level, inflammatory factor expression, and activation of MRGPRX2/ORAI axis in the DNFB-induced atopic dermatitis model. The influence of Celastrol on atopic dermatitis was remarkably reversed by overexpression of MRGPRX2. Conclusion. We found that the improvements of atopic dermatitis caused by Celastrol were reversed by treatment with MRGPRX2OE, indicating that Celastrol might affect atopic dermatitis through MRGPRX2. This study might provide a novel thought for the prevention and treatment of atopic dermatitis by regulating MRGPRX2.

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Section: Introductionmentioning

confidence: 99%

Inhibition of Mast Cell Degranulation in Atopic Dermatitis by Celastrol through Suppressing MRGPRX2

Yao

Chen

2023

Disease Markers

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“…Several outstanding reviews have discussed the roles of mouse and human basophils in allergic disorders ( 1 , 64 , 74 , 81 , 82 ) and parasitic infections ( 66 – 68 ). Increasing evidences indicate that basophils also play relevant roles in several other types of responses, including autoimmunity ( 83 , 84 ), myocardial infarction ( 70 ), fibrosis ( 70 , 71 , 85 ), cancer ( 86 – 88 ), and COVID-19 ( 89 ). In this review, we discuss the recent basophil contribution to the pathogenesis of several non-allergic inflammatory diseases.…”

Section: Basic Concepts Of Basophilsmentioning

confidence: 99%

Basophils beyond allergic and parasitic diseases

et al. 2023

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Basophils bind IgE via FcεRI-αβγ2, which they uniquely share only with mast cells. In doing so, they can rapidly release mediators that are hallmark of allergic disease. This fundamental similarity, along with some morphological features shared by the two cell types, has long brought into question the biological significance that basophils mediate beyond that of mast cells. Unlike mast cells, which mature and reside in tissues, basophils are released into circulation from the bone marrow (constituting 1% of leukocytes), only to infiltrate tissues under specific inflammatory conditions. Evidence is emerging that basophils mediate non-redundant roles in allergic disease and, unsuspectingly, are implicated in a variety of other pathologies [e.g., myocardial infarction, autoimmunity, chronic obstructive pulmonary disease, fibrosis, cancer, etc.]. Recent findings strengthen the notion that these cells mediate protection from parasitic infections, whereas related studies implicate basophils promoting wound healing. Central to these functions is the substantial evidence that human and mouse basophils are increasingly implicated as important sources of IL-4 and IL-13. Nonetheless, much remains unclear regarding the role of basophils in pathology vs. homeostasis. In this review, we discuss the dichotomous (protective and/or harmful) roles of basophils in a wide spectrum of non-allergic disorders.

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“…Depending on the clinicians’ extensive experiences, some patients are missed or misdiagnosed. The sensitivity of diagnosis is enhanced when combined with serology results ( Poto et al, 2022 ). Thus, a comprehensive evaluation of combination clinic symptoms with serology gradually attracts attention.…”

Section: Introductionmentioning

confidence: 99%

bSRWPSO-FKNN: A boosted PSO with fuzzy K-nearest neighbor classifier for predicting atopic dermatitis disease

Zhao²,

Xu³

et al. 2023

Front. Neuroinform.

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IntroductionAtopic dermatitis (AD) is an allergic disease with extreme itching that bothers patients. However, diagnosing AD depends on clinicians’ subjective judgment, which may be missed or misdiagnosed sometimes.MethodsThis paper establishes a medical prediction model for the first time on the basis of the enhanced particle swarm optimization (SRWPSO) algorithm and the fuzzy K-nearest neighbor (FKNN), called bSRWPSO-FKNN, which is practiced on a dataset related to patients with AD. In SRWPSO, the Sobol sequence is introduced into particle swarm optimization (PSO) to make the particle distribution of the initial population more uniform, thus improving the population’s diversity and traversal. At the same time, this study also adds a random replacement strategy and adaptive weight strategy to the population updating process of PSO to overcome the shortcomings of poor convergence accuracy and easily fall into the local optimum of PSO. In bSRWPSO-FKNN, the core of which is to optimize the classification performance of FKNN through binary SRWPSO.ResultsTo prove that the study has scientific significance, this paper first successfully demonstrates the core advantages of SRWPSO in well-known algorithms through benchmark function validation experiments. Secondly, this article demonstrates that the bSRWPSO-FKNN has practical medical significance and effectiveness through nine public and medical datasets.DiscussionThe 10 times 10-fold cross-validation experiments demonstrate that bSRWPSO-FKNN can pick up the key features of AD, including the content of lymphocytes (LY), Cat dander, Milk, Dermatophagoides Pteronyssinus/Farinae, Ragweed, Cod, and Total IgE. Therefore, the established bSRWPSO-FKNN method practically aids in the diagnosis of AD.

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IgG Autoantibodies Against IgE from Atopic Dermatitis Can Induce the Release of Cytokines and Proinflammatory Mediators from Basophils and Mast Cells

Cited by 16 publications

References 105 publications

Inhibition of Mast Cell Degranulation in Atopic Dermatitis by Celastrol through Suppressing MRGPRX2

Inhibition of Mast Cell Degranulation in Atopic Dermatitis by Celastrol through Suppressing MRGPRX2

Basophils beyond allergic and parasitic diseases

bSRWPSO-FKNN: A boosted PSO with fuzzy K-nearest neighbor classifier for predicting atopic dermatitis disease

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